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Journals / Alatarım / 2011 / Cilt: 10 - Sayı: 2

Determination of resistance to fusarium wilt race 1 of watermelon by P01-700 SCAR marker

Karpuz fusarium solgunluk etmeni Fusarium oxysporum f. sp. niveum' un 1 nolu ırkına dayanıklılığının P01-700 SCAR markörü yardımıyla belirlenmesi

Hasan PINAR
Veysel ARAS
Davut KELEŞ
MUSTAFA ÜNLÜ
Nedim MUTLU
Journal
Alatarım
Pages
57–62
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that this marker can be used in breeding programs for resistance against Fusarium among segregating populations where resistance originates from PI 296341-FR.

📌 3 Key Takeaways

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Objective & Hypothesis:Fusarium oxysporum f. sp. niveum (Fon) is one of the most important factors which limits to production of watermelon (Citrullus lanatus (Thumb.) Matsum & Nakai).
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Key Findings & Data:In watermelon breeding, screening can be made using molecular methods via P01-700 SCAR marker for Fon resistance originating from genotype PI 296341-FR.
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Conclusion & Impact:The results showed that this marker can be used in breeding programs for resistance against Fusarium among segregating populations where resistance originates from PI 296341-FR.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:karpuz · bitki ıslahı · markör gen

Abstract

Fusarium oxysporum f. sp. niveum (Fon) is one of the most important factors which limits to production of watermelon (Citrullus lanatus (Thumb.) Matsum & Nakai). One of the best ways to the fight against Fon is to develope resistant watermelon cultivars or lines. In watermelon breeding, screening can be made using molecular methods via P01-700 SCAR marker for Fon resistance originating from genotype PI 296341-FR. In this study, $f_{2}$ populations and backcross population $BC_1 F_1$ of Alata136 (sensitive) x PI 296341-FR (resistant) were screened via P01-700 SCAR for marker-assisted selection. The 30 of 42 F2 individuals and 52 of 96 backcross individuals yielded the expected band at 730 bp for Fon resistance in watermelon populations. According to$X^2$ test, segregation ratios in $f_{2}$ and backcross populations were 3:1 and 1:1, respectively. The results showed that this marker can be used in breeding programs for resistance against Fusarium among segregating populations where resistance originates from PI 296341-FR.

Özet

Fusarium oxysporum f. sp. niveum (Fon) karpuz Citrullus lanatus (Thumb.) Matsum & Nakai) üretimini sınırlayan en önemli faktörlerden birisidir. Fon ile mücadelede en iyi yollardan birisi dayanıklı karpuz hat veya çeşitlerinin geliştirilmesidir. Fon’a karşı PI 296341-FR kaynaklı dayanıklılık geni P01-700 SCAR markörü ile dağılım gösteren populasyonlarda takip edilebilmektedir. Bu çalışmada Alata136 (hassas) x PI 296341-FR (dayanıklı) melezlerinin,kendilenmesi $f_{2}$ ve geriye melezlenmesi (GM1F1) ile elde edilen populasyonlar, P01-700 SCAR markör yardımlı seleksiyona tabi tutulmuştur. 42 adet F2 bireyinin 30 adedinde ve 96 adet $GM_1 F_1$ bireyinin 52 adedinde 730 baz çifti dayanıklılık markör bandı elde edilmiştir. Hesaplanan $X^2$ testine göre tek lokustan beklendiği gibi$f_{2}$ populasyonunda 3:1 açılım oranı,$GM_1 F_1$ populasyonunda ise 1:1 açılım oranı görülmüştür. Elde edilen bulgular, PI 296341-FR kaynaklı Fusariuma dayanıklılık geninin hassas karpuz genotiplerine aktarılmasında SCAR markörünün moleküler markör yardımlı seleksiyon amacıyla kullanılabileceğini göstermiştir.

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Cite This Article

PINAR, H., ARAS, V., KELEŞ, D., ÜNLÜ, M., MUTLU, N. (2011). Determination of resistance to fusarium wilt race 1 of watermelon by P01-700 SCAR marker. Alatarım, Cilt: 10 - Sayı: 2, 57–62. https://ajindex.com/articles/147496

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