SavedAdd your journal

Journals / Bulletin of Biotechnology / 2026 / Volume: 7 Issue: 1

Comparative Analysis of Radish Varieties: Effects on Sugar Metabolism and Antioxidant Enzymes

Feyza Akgün(SELÇUK ÜNİVERSİTESİ)
Nuraniye Eruygur(SELÇUK ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0002-4674-7009
Pages
—
Publication Date
2026-06-29
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, red radish (FKT) emerged as the most promising candidate among the three varieties, exhibiting the highest levels of bioactive compounds and the strongest antidiabetic and antioxidant activities.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study investigated the in vitro antidiabetic and antioxidant properties of three radish varieties—white radish (FBT), red radish (FKT), and black radish (FST)—sourced from Konya.
📊
Key Findings & Data:Antioxidant capacity was comprehensively assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and iron chelation activity (FCA) assays.
💡
Conclusion & Impact:In conclusion, red radish (FKT) emerged as the most promising candidate among the three varieties, exhibiting the highest levels of bioactive compounds and the strongest antidiabetic and antioxidant activities.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Radish · enzyme inhibition · antioxidant

Abstract

This study investigated the in vitro antidiabetic and antioxidant properties of three radish varieties—white radish (FBT), red radish (FKT), and black radish (FST)—sourced from Konya. The evaluation encompassed several key biological activities. Total flavonoid content (TFC) and total phenolic content (TPC) were measured to establish the phytochemical composition. Antioxidant capacity was comprehensively assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and iron chelation activity (FCA) assays. Antidiabetic activity was determined by quantifying the inhibition of the α-amylase and α-glucosidase enzymes. Among the samples, red radish (FKT) exhibited the highest TFC (1.27 mg KE/g) and TPC (55.36 mg GAE/g), followed by black radish (FST) and white radish (FBT). Antioxidant activity, assessed via DPPH, ABTS, and iron chelation assays, revealed that FST had the strongest DPPH radical scavenging activity (84.32%; IC₅₀ = 636.6 μg/mL), while FKT showed superior ABTS inhibition (83.79%; IC₅₀ = 144.5 μg/mL) and FBT iron chelation capacity (74.04%; IC₅₀ = 677.2 μg/mL). In terms of antidiabetic potential, FKT demonstrated the most potent α-amylase inhibition (97.36%; IC₅₀ = 2.07 mg/mL) and α-glucosidase inhibition (84.18%), indicating strong enzyme inhibitory activity. FBT showed moderate activity on most assays, while FST displayed notable antioxidant properties but comparatively lower antidiabetic effects. In conclusion, red radish (FKT) emerged as the most promising candidate among the three varieties, exhibiting the highest levels of bioactive compounds and the strongest antidiabetic and antioxidant activities. These findings suggest that FKT may offer valuable health benefits and could be further explored for its therapeutic potential in managing oxidative stress and diabetes-related conditions.

🔖

Cite This Article

Akgün, F., Eruygur, N. (2026). Comparative Analysis of Radish Varieties: Effects on Sugar Metabolism and Antioxidant Enzymes. Bulletin of Biotechnology, Volume: 7 Issue: 1. https://doi.org/10.51539/biotech.1917286

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (30)

  1. Alkan B, Emre N, Gökcüoğlu S, Özşahin A, Edirne, T (2024) An Assessment of the Use of Conventional and Complementary Therapies Among Patients Diagnosed with Type 2 Diabetes Mellitus. Mersin University Faculty of Medicine Lokman Hekim Journal of the History of Medicine and Folk Medicine,14(3): 579-586.
  2. Beevi SS, Mangamoori LN, Gowda BB (2012) Polyphenolics profile and antioxidant properties of Raphanus sativus L. Natural Product Research, 26(6): 557-563.
  3. Brand-Williams W, Cuvelier ME, Berset, C (1995) Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28(1): 25-30.
  4. Chai T, Mohan M, Ong H, Wong F (2014) Antioxidant, iron-chelating and anti-glucosidase activities of Typha domingensis Pers (Typhaceae). Tropical Journal of Pharmaceutical Research, 13(1): 67-72.
  5. Cheplick S, Kwon YI, Bhowmik, P, Shetty K (2010) Phenolic-linked variation in strawberry cultivars for potential dietary management of hyperglycemia and related complications of hypertension. Bioresource Technology, 101(1): 404-413.
  6. Dewanto V, Wu X, Adom KK, Liu, R H (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of agricultural and food chemistry, 50(10): 3010-3014.
  7. Farid, MM, Ibrahim FM, Ragheb AY, Mohammed RS, Hegazi NM, Shabrawy MOE, Kawashty SA, Marzouk MM (2022) Comprehensive phytochemical characterization of Raphanus raphanistrum L.: in vitro antioxidant and antihyperglycemic evaluation. Scientific African, 16: e01154.
  8. Ferreira ICF R, Morales P, Barros L (eds) (2017) Wild plants, mushrooms and nuts: functional food properties and applications. John Wiley & Sons, Chichester
  9. Folli F, Corradi D, Fanti P, Davalli A, Paez A, Giaccari A, Perego C, Muscogiuri G (2011) The role of oxidative stress in the pathogenesis of type 2 diabetes mellitus micro-and macrovascular complications: avenues for a mechanistic-based therapeutic approach. Current diabetes reviews, 7(5): 313-324.
  10. Forbes JM, Cooper ME (2013) Mechanisms of diabetic complications. Physiological reviews, 93(1): 137-188.
  11. Forman HJ, Zhang H (2021) Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery, 20(9): 689-709.
  12. Gao L, Li H, Li B, Shao H, Yu X, Miao Z, Zhan L, Zhu L, Sheng H (2022) Traditional uses, phytochemistry, transformation of ingredients and pharmacology of the dried seeds of Raphanus sativus L.(Raphani Semen): A comprehensive review. Journal of ethnopharmacology, 294: 115387.
  13. Gökpınar Ş, Koray T, Akçiçek E, Göksan T, Durmaz Y (2006) Algal antioksidanlar. Su Ürünleri Dergisi, 23(1): 85-89.
  14. Haidara, Mikhailidis DP, Rateb MA, Ahmed ZA, Yassin HZ, Ibrahim IM, Rashed LA (2009) Evaluation of the effect of oxidative stress and vitamin E supplementation on renal function in rats with streptozotocin-induced Type 1 diabetes. Journal of Diabetes and its Complications, 23(2): 130-136.
  15. Hanlon PR, Barnes DM (2011) Phytochemical composition and biological activity of 8 varieties of radish (Raphanus sativus L.) sprouts and mature taproots. Journal of Food Science, 76(1): C185-C192.
  16. Jugran AK, Rawat S, Devkota HP, Bhatt ID, Rawal RS (2021) Diabetes and plant‐derived natural products: From ethnopharmacological approaches to their potential for modern drug discovery and development. Phytotherapy Research, 35(1): 223-245.
  17. Kajszczak D, Sosnowska D, Frąszczak B, Podsędek, A (2024) Composition, Anti-Diabetic, and Antioxidant Potential of Raphanus sativus Leaves. Molecules, 29(23): 5689.
  18. Karabulut H, Gülay MŞ (2016) Antioksidanlar. Veterinary Journal of Mehmet Akif Ersoy University, 1(1): 65-76.
  19. Kim KH, Moon E, Kim SY, Choi SU, Lee JH, Lee KR (2014) 4-Methylthio-butanyl derivatives from the seeds of Raphanus sativus and their biological evaluation on anti-inflammatory and antitumor activities. Journal of ethnopharmacology, 151(1): 503-508.
  20. Kim SH, Dhungana SK, Kim ID, Adhikari A, Kim JH (2024) Effect of Illite Treatment on Quality Characteristics and Antioxidant Activity of Broccoli (Brassica oleracea L. var. italica) Sprouts. Molecules, 29(18): 4347.
  21. Kucukatay V, Ağar A, Gumuslu S, Yargiçoğlu P (2007) Effect of sulfur dioxide on active and passive avoidance in experimental diabetes mellitus: relation to oxidant stress and antioxidant enzymes. International Journal of Neuroscience, 117(8): 1091-1107.
  22. Kwon YI, Apostolidis E, Shetty K (2008) Inhibitory potential of wine and tea against α‐amylase and α‐glucosidase for management of hyperglycemia linked to type 2 diabetes. Journal of Food Biochemistry, 32(1): 15-31.
  23. Lee J, Noh S, Lim S, Kim B (2021) Plant extracts for type 2 diabetes: From traditional medicine to modern drug discovery. Antioxidants, 10(1): 81.
  24. Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews, 4(8): 118.
  25. Önder FC, Doğrular N, Güzdüzalp E, Barlak S, Ay M (2020) A comparative study of three Brassicaceae vegetables grown in Canakkale: Determination of total phenolic content and antioxidant activity of pulp and juice samples of radish (Raphanus sativus L.), cabbage (Brassica oleracea L. var capitata L.) and cauliflower (Brassica oleracea L.). Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 6(1): 30-38.
  26. Park CH, Baskar TB, Park SY, Kim SJ, Valan Arasu M, Al-Dhabi NA, Kim JK, Park SU (2016) Metabolic profiling and antioxidant assay of metabolites from three radish cultivars (Raphanus sativus). Molecules, 21(2): 157.
  27. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10): 1231-1237. Sarıkamış G, Yıldırım A, Alkan D (2015). Glucosinolates in seeds, sprouts and seedlings of cabbage and black radish as sources of bioactive compounds. Canadian Journal of Plant Science, 95(4): 681-687.
  28. Vl S, (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299: 152-178.
  29. Xu W, Caracciolo B, Wang HX, Winblad B, Bäckman L, Qiu C,Fratiglioni L (2010) Accelerated progression from mild cognitive impairment to dementia in people with diabetes. Diabetes, 59(11): 2928-2935.
  30. Yılmaz, B, Karabudak E (2018) Diet-Induced Advanced Glycation End Products and Their Effects on Health. Acıbadem University Journal of Health Sciences, (4): 349-356.