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Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 3

Isolation of Clostridium perfringens from Chickens and Detection of the Alpha Toxin Gene by Polymerase Chain Reaction (PCR)

Hakan KALENDER
Pages
847–851
Publication Date
2005-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Since C. perfringens type A is associated with food poisoning in humans, the isolation of C. perfringens type A from chickens in this study is important for public health.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR).
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Key Findings & Data:Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed.
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Conclusion & Impact:Since C. perfringens type A is associated with food poisoning in humans, the isolation of C. perfringens type A from chickens in this study is important for public health.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Chicken · Clostridium perfringens · isolation

Abstract

This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR). Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed. C. perfringens was isolated from 8 (5%) of the samples. DNA samples extracted from suspected isolates grown on selective agar were amplified by PCR using a pair of primers derived from the alpha toxin gene. All of 8 suspected isolates were found to be C. perfringens by conventional methods and PCR. Isolates were typed by the toxin neutralization test. Of the 8 isolates, 6 were type A and 2 could not be typed. Since C. perfringens type A is associated with food poisoning in humans, the isolation of C. perfringens type A from chickens in this study is important for public health.

Özet

This study was carried out to isolate Clostridium perfringens from chickens and to detect the gene encoding the alpha toxin produced by all types of C. perfringens by polymerase chain reaction (PCR). Intestinal contents of 160 slaughtered chickens from 8 different farms in Elazığ province were analyzed. C. perfringens was isolated from 8 (5%) of the samples. DNA samples extracted from suspected isolates grown on selective agar were amplified by PCR using a pair of primers derived from the alpha toxin gene. All of 8 suspected isolates were found to be C. perfringens by conventional methods and PCR. Isolates were typed by the toxin neutralization test. Of the 8 isolates, 6 were type A and 2 could not be typed. Since C. perfringens type A is associated with food poisoning in humans, the isolation of C. perfringens type A from chickens in this study is important for public health.

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Cite This Article

KALENDER, H. (2005). Isolation of Clostridium perfringens from Chickens and Detection of the Alpha Toxin Gene by Polymerase Chain Reaction (PCR). Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 3, 847–851. https://ajindex.com/articles/146355

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