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Journals / Aksaray Üniversitesi Tıp Bilimleri Dergisi / 2026 / Cilt: 6 Sayı: 2

Comparative Evaluation of Brucella Immunocapture Agglutination and Coombs Gel Tests in the Serological Diagnosis of Human Brucellosis

İnsan brusellozunun serolojik tanısında brucella immunocapture aglütinasyon ve coombs jel testlerinin karşılaştırmalı değerlendirilmesi

Firdevs Güvenç(Aksaray eğitim ve araştırma hastanesi)0000-0002-3522-3293
Selçuk Türkel(AKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ MİKROBİYOLOJİ ANABİLİM DALI)0000-0001-5392-8679
Altan Akıneden(AKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ MİKROBİYOLOJİ ANABİLİM DALI)0000-0002-1434-6892
Yücel Duman(AKSARAY ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ MİKROBİYOLOJİ ANABİLİM DALI)0000-0002-9090-2096
Pages
46–50
Publication Date
2026-05-18
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Results: A very high level of concordance was observed between the two tests, with an overall agreement of 96.0 % (κ = 0.92).

📌 3 Key Takeaways

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Objective & Hypothesis:ABSTRACT Objective: This study aimed to compare the diagnostic performance and agreement between the Brucella Immunocapture Agglutination Test (Brucella Capt, BICA) and the Brucella Coombs Gel Test (BCGT) in the serological diagnosis of human brucellosis.
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Key Findings & Data:Materials and Methods: Serum samples from 51 Rose Bengal Test (RBT)–positive and 50 RBT–negative patients with a preliminary diagnosis of brucellosis were analyzed using the BICA and BCGT assays.
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Conclusion & Impact:Results: A very high level of concordance was observed between the two tests, with an overall agreement of 96.0 % (κ = 0.92).
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Bruselloz · Brucella Capt · Brucella Coombs Jel Testi

Abstract

ABSTRACT Objective: This study aimed to compare the diagnostic performance and agreement between the Brucella Immunocapture Agglutination Test (Brucella Capt, BICA) and the Brucella Coombs Gel Test (BCGT) in the serological diagnosis of human brucellosis. Materials and Methods: Serum samples from 51 Rose Bengal Test (RBT)–positive and 50 RBT–negative patients with a preliminary diagnosis of brucellosis were analyzed using the BICA and BCGT assays. The agreement between the two methods was evaluated using Cohen’s kappa (κ) coefficient, and positive and negative percent agreements (PPA, NPA) were calculated. Results: A very high level of concordance was observed between the two tests, with an overall agreement of 96.0 % (κ = 0.92). The PPA and NPA were 95.9 % and 100 %, respectively. A substantial correlation was also found between antibody titers of the two assays (weighted κ = 0.64). Conclusion: Brucella Capt (BICA) and Brucella Coombs Gel Test (BCGT) demonstrated substantial agreement and comparable diagnostic performance in the serological diagnosis of brucellosis. The complementary use of these assays may be particularly beneficial in clinically challenging situations, such as cases with low antibody titers or atypical serological profiles. Integration of these methods with clinical evaluation and, when available, molecular or culture-based approaches may further enhance diagnostic confidence.

Özet

ÖZ Amaç: Bu çalışmada insan brusellozunun serolojik tanısında kullanılan Brucella İmmünokap¬tür Aglütinasyon Testi (Brucella Capt, BICA) ile Brucella Coombs Jel Testi’nin (BCGT) tanısal performanslarının ve aralarındaki uyumun karşılaştırılması amaçlanmıştır. Gereç ve Yöntem: Bruselloz ön tanısı alan 51 Rose Bengal Testi (RBT) pozitif ve 50 RBT negatif hastaya ait serum örnekleri BICA ve BCGT testleriyle analiz edilmiştir. Testler arasındaki uyum Cohen’s kappa (κ) katsayısı ile değerlendirilmiş; pozitif ve negatif yüzde uyum (PPA, NPA) oranları hesaplanmıştır. Bulgular: İki test arasında çok yüksek düzeyde uyum saptanmıştır (genel uyum %96.0, κ = 0.92). Pozitif ve negatif uyum oranları sırasıyla %95.9 ve %100 olarak bulunmuştur. Antikor titreleri arasında da anlamlı bir ilişki belirlenmiştir (ağırlıklı κ = 0.64). Sonuç: Brucella Capt (BICA) ve Brucella Coombs Jel Testi (BCGT), brusellozun serolojik tanısında yüksek düzeyde uyum ve karşılaştırılabilir tanısal performans göstermiştir. Bu testlerin birlikte kullanımı, özellikle düşük antikor titreleri veya atipik serolojik profillerin bulunduğu klinik olarak zorlayıcı durumlarda tanısal değerlendirmeye katkı sağlayabilir. Klinik bulgularla birlikte ve olanaklar dâhilinde moleküler veya kültür temelli yaklaşımlarla desteklenmesi, tanısal güvenilirliği daha da artırabilir.

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Cite This Article

Güvenç, F., Türkel, S., Akıneden, A., Duman, Y. (2026). Comparative Evaluation of Brucella Immunocapture Agglutination and Coombs Gel Tests in the Serological Diagnosis of Human Brucellosis. Aksaray Üniversitesi Tıp Bilimleri Dergisi, Cilt: 6 Sayı: 2, 46–50. https://ajindex.com/articles/1447366

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References (13)

  1. Christopher S. Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. J Lab Physicians. 2010;2(2):55–60.
  2. Xu N, Qu C, Sai L, Wen S, Yang L, Wang S, et al. Evaluating the efficacy of serological testing of clinical specimens collected from patients with suspected brucellosis. PLoS Negl Trop Dis. 2023;17(2):e0011131.
  3. Orduña A, Almaraz A, Prado A, Gutierrez MP, Garcia-Pascual A, Dueñas A, et al. Evaluation of an immunocapture-agglutination test (Brucellacapt) for serodiagnosis of human brucellosis. J Clin Microbiol. 2000;38(11):4000–40005.
  4. İrvem A, Yücel FM, Aksaray S, Bor E. Comparison of a new and rapid method, Brucella Coombs Gel Test, with other methods in the serological diagnosis of brucellosis. Mikrobiyol Bul. 2015;49(2):181–187.
  5. Erdin BN, Parlak M, Güven Aydınlı A, Demir F. Brucella Coombs Gel Test: can it replace other serological methods as a rapid test in serological diagnosis of brucellosis? Turk Hij Den Biyol Derg. 2021;78(3):249–254.
  6. Casanova A, Ariza J, Rubio M, Masuet C, Díaz R, Montes M, et al. Brucellacapt versus classical tests in the serological diagnosis of human brucellosis. Clin Vaccine Immunol. 2009;16(6):844–851.
  7. Koçman EE, Kaya S, Demir C, Kuloğlu F, Erensoy MS, Çiçeklioğlu M. Comparison of standard agglutination tests, enzyme-linked immunosorbent assay and Brucella Coombs Gel Test (BCGT) in diagnosis of brucellosis. Turk J Med Sci. 2018;48(1):62–67.
  8. Bosilkovski M, Spasovska K, Sopova Z, Vidinic I. The role of Brucellacapt test for follow-up patients with brucellosis. Comp Immunol Microbiol Infect Dis. 2010;33(5):435–442.
  9. Loubet P, Magnan C, Salipante F, Pastre T, Keriel A, O’Callaghan D, et al. Diagnosis of brucellosis: combining tests to improve performance. PLoS Negl Trop Dis. 2024;18(9):e0012442.
  10. Elbehiry A, Aldubaib M, Marzouk E, Abalkhail A, Almuzaini AM, Rawway M, et al. Development of diagnostic and vaccine strategies for early detection and control of human brucellosis, particularly in endemic areas. Vaccines (Basel). 2023;11(3):654.
  11. Kaya M, Kurtoğlu MG, Güzelant A, Güvenç Hİ, Övet H, Akkaya O, et al. Brucella Jel Aglütinasyon Testinin Brusella tanısında kullanılan diğer serolojik testlerle karşılaştırılması. Selcuk Univ Med J. 2018;34(1):1–5.
  12. Ay S, Güzel B, Duman Y. Investigation of compatibility between serological tests used in laboratory diagnosis of brucellosis. Acta Med Alanya. 2021;5(2):171–174.
  13. Shi F, Tang Y, Xu ZH, Sun YX, Ma MZ, Chen CF. Visual typing detection of brucellosis with a lateral flow immunoassay based on coloured latex microspheres. J Appl Microbiol. 2022;132(1):199–208.