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Journals / Journal of Experimental and Clinical Medicine / 2026 / Volume: 43 Issue: 1

Electrophysiological and histological investigation of the effect of interleukin-1 receptor antagonist on acute and PTZ kindling model of experimental epilepsy

Hayriye Soytürk(BOLU ABANT IZZET BAYSAL UNIVERSITY)0000-0002-0000-3768
Ümit Kılıç(AFYONKARAHISAR HEALTH SCIENCES UNIVERSITY, FACULTY OF HEALTH SCIENCES)0000-0001-9917-0648
Ayşegül Yıldız0000-0002-4975-2233
Ömür Gülsüm Deniz(BOLU ABANT IZZET BAYSAL UNIVERSITY)0000-0001-9254-175X
Eylem Suveren(BOLU ABANT IZZET BAYSAL UNIVERSITY)0000-0003-1730-6463
Mehtap Ayvacı0000-0001-6225-2367
Şerif Demir(DÜZCE ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0002-0305-5758
Pages
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Publication Date
2026-04-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings indicate that IL-1Ra exerts phase-dependent effects in experimental epilepsy.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to electrophysiologically and histopathologically evaluate the effects of the interleukin-1 receptor antagonist (IL-1Ra) on epileptogenesis in acute and chronic pentylenetetrazole (PTZ)-induced seizure models.
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Key Findings & Data:A total of 42 male rats were divided into seven groups, including acute PTZ and PTZ kindling models.
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Conclusion & Impact:These findings indicate that IL-1Ra exerts phase-dependent effects in experimental epilepsy.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Neuroinflammation · Epilepsy · IL-1Ra

Abstract

This study aimed to electrophysiologically and histopathologically evaluate the effects of the interleukin-1 receptor antagonist (IL-1Ra) on epileptogenesis in acute and chronic pentylenetetrazole (PTZ)-induced seizure models. A total of 42 male rats were divided into seven groups, including acute PTZ and PTZ kindling models. The effects of IL-1Ra (50 mg/kg, intraperitoneal) on seizure activity were assessed using electrocorticographic recordings, analyzing total seizure number, spike-wave number, and spike-wave duration. Histopathological examinations were performed to evaluate neuronal integrity. In the acute PTZ model, IL-1Ra administration resulted in a significant increase in the total number of seizures compared with the PTZ group (p < 0.05), while spike-wave duration was reduced. In contrast, in the PTZ kindling model, IL-1Ra decreased seizure frequency and total spike-wave duration (p < 0.05). Histopathological analysis revealed improved neuronal morphology in both the PTZ+Diazepam and PTZ+IL-1Ra groups compared with the acute PTZ group. These findings indicate that IL-1Ra exerts phase-dependent effects in experimental epilepsy. While early IL-1 receptor blockade may aggravate seizure frequency in the acute phase, it appears to attenuate epileptiform activity and provide partial neuroprotection in the chronic stage of epileptogenesis. The results underscore the importance of timing and inflammatory context in cytokine-targeted therapeutic strategies.

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Cite This Article

Soytürk, H., Kılıç, Ü., Yıldız, A., Deniz, Ö. G., Suveren, E., Ayvacı, M., Demir, Ş. (2026). Electrophysiological and histological investigation of the effect of interleukin-1 receptor antagonist on acute and PTZ kindling model of experimental epilepsy. Journal of Experimental and Clinical Medicine, Volume: 43 Issue: 1. https://ajindex.com/articles/1422526

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