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Journals / Journal of Experimental and Clinical Medicine / 2026 / Volume: 43 Issue: 2

Bifidobacterium animalis subsp. animalis-derived metabolites are implicated in microbiota–tumor crosstalk through dual anticancer and antimicrobial effects in colorectal cancer

Pinar Sari Yolsel(NISANTASI UNIVERSITY)0000-0003-0246-5541
Kamil Serkan Uzyol(İSTANBUL NİŞANTAŞI ÜNİVERSİTY)0000-0001-6436-9083
Özlem Ateş Duru(BOLU ABANT IZZET BAYSAL UNIVERSITY)0000-0001-8961-5113
Muhammet Volkan Bülbül(AGRI IBRAHIM CECEN UNIVERSITY)0000-0003-1526-2065
Semiha Mervenur Kalender(İSTANBUL MEDİPOL UNİVERSİTY)0000-0002-0885-3417
Özge Çelik(İSTANBUL KÜLTÜR ÜNİVERSİTY)0000-0002-9565-5135
Pages
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Publication Date
2026-06-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings suggest B. animalis-derived metabolites exert direct anticancer effects and indirectly modulate the tumor microenvironment by inhibiting pathogenic bacterial colonization.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Colorectal cancer constitutes a considerable global health challenge due to its strong contribution to cancer-linked morbidity and mortality.
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Key Findings & Data:This study examined the effects of extracellular metabolites from B. animalis subsp. animalis on human CRC cell lines (HCT-116 and HT-29) and on pathogenic bacteria (Escherichia coli and Staphylococcus aureus).
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Conclusion & Impact:These findings suggest B. animalis-derived metabolites exert direct anticancer effects and indirectly modulate the tumor microenvironment by inhibiting pathogenic bacterial colonization.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bifidobacterium animalis · colorectal carcinoma · Escherichia coli

Abstract

Colorectal cancer constitutes a considerable global health challenge due to its strong contribution to cancer-linked morbidity and mortality. Gut microbiota and their metabolites are increasingly recognized as modulators of tumor progression and the tumor microenvironment. This study examined the effects of extracellular metabolites from B. animalis subsp. animalis on human CRC cell lines (HCT-116 and HT-29) and on pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Metabolite exposure produced dose-dependent inhibition of CRC cell viability, with IC₅₀ values of 63.92 µL/mL for HCT-116 and 46.86 µL/mL for HT-29, while causing minimal cytotoxicity in non-tumorigenic HUVEC cells. Flow cytometry indicated a modest rise in late apoptotic populations, suggesting activation of apoptotic pathways without extensive cell death. Antimicrobial assays showed effective, time- and dose-dependent suppression of E. coli and S. aureus growth under aerobic and anaerobic conditions. These findings suggest B. animalis-derived metabolites exert direct anticancer effects and indirectly modulate the tumor microenvironment by inhibiting pathogenic bacterial colonization.

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Cite This Article

Yolsel, P. S., Uzyol, K. S., Duru, Ö. A., Bülbül, M. V., Kalender, S. M., Çelik, Ö. (2026). Bifidobacterium animalis subsp. animalis-derived metabolites are implicated in microbiota–tumor crosstalk through dual anticancer and antimicrobial effects in colorectal cancer. Journal of Experimental and Clinical Medicine, Volume: 43 Issue: 2. https://ajindex.com/articles/1422511

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