In Vitro Shoot Regeneration of Common Vetch (Vicia sativa L.) Using Immature Cotyledons

Abstract

Common vetch (Vicia sativa L.) is multi usage legume as a cover crop, green manure, pasture, silage, and hay due to its high dry matter and nitrogen accumulation. It is neglected crop due to toxicity to non-ruminant animals including humans. It causes a disease called favisim due to the presence of an oxidants like convicine, isouramil, divicine and vicine which results in lowering of glutathione levels in G6PD-deficient persons. Immature cotyledon seeds were cultured on MS medium containing 0.05-0.80 mg/l TDZ-0.10 mg/l IBA. Callusing without shoot regeneration was observed only on higher concentarion of 0.80 mg/l TDZ-0.10 mg/l IBA in the culture medium. Shoot regeneration frequency and shoots per explants ranged 25.0-58.33% and 8.33-19.33 respectively. Maximum Shoot regeneration frequency (58.33%) and shoots per explants (19.33) were recorded on MS medium supplemented with 0.20 mg/l TDZ-0.10 mg/l IBA. Equal concentration of TDZ-IBA induced maximum shoot length and was recorded 5.0 cm. Most of the palnts rooted directly in the culture medium and remaining were cultured on MS medium containing 1.0 mg/l IBA. Both types of plantlets were acclimatized under ambient conditions