Molecular Identification of Glomus mosseae from Wheat Rhizosphere in Damghan Region, Iran

Abstract

Arbuscular mycorrhizas (AM) have mutualistic symbiosis with plants and thus efforts have been placed on application of these symbiotic relationships to agricultural and environmental fields. In this study, soil and root samples were collected from 40 sites of wheat rhizospheres in Damghan region located in central parts of Iran. Fungal structures as well as colonization were observed by root staining and clearing. Also, trap cultures established using maize in greenhouse condition. AM fungal spores were extracted and identified using morphological method. Using morphological characters, 12 species of what were identified. The most abundant species was G. mosseae. DNA was extracted from different plant and fungal structures including colonized and non-colonized roots, sporocarps, extraradical mycelia as well as single spores. Partial regions on 18S rDNA as well as 28S rDNA were amplified using PCR with AM fungal specific primers (AML1/NS31) and G. mosseae specific primers (NDL22/5.21)