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Journals / Alatarım / 2018 / Cilt: 17 - Sayı: 1

Optimization of Plant Regeneration from Root Explants of Carrizo citrange (Citrus sinensis Osb. X Poncirus trifoliata Raf. var “Carrizo”)

Carrizo Sitranjı (Citrus sinensis Osb. X Poncirus trifoliata Raf. var “Carrizo”)'nın Kök Eksplantlarından Bitki Rejenerasyonunun Optimizasyonu

Ülkü İNCEOĞLU
Dicle DÖNMEZ
ÖZHAN ŞİMŞEK
TURGUT YEŞİLOĞLU
Yıldız Aka KAÇAR
Journal
Alatarım
Pages
18–26
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In shoot formation, 100% success was achieved in MS media containing 0.5 mg/L BA, 1 mg/L BA+0.5 mg/L NAA or 2 mg/L BA.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The diversity and economic value of citrus encourages study of plant biotechnology in addition to classical plant breeding.
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Key Findings & Data:Root explants of Carrizo citrange were cultured in MS nutrient medium containing 21 different concentrations of BA (6-Benzyl amino purine) and NAA (1-Naphthaleneacetic acid).
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Conclusion & Impact:In shoot formation, 100% success was achieved in MS media containing 0.5 mg/L BA, 1 mg/L BA+0.5 mg/L NAA or 2 mg/L BA.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The diversity and economic value of citrus encourages study of plant biotechnology in addition to classical plant breeding. In this study, optimization of plant regeneration was aimed at using root explants developed from the seeds of Carrizo citrange germinated in in vitro conditions. Root explants of Carrizo citrange were cultured in MS nutrient medium containing 21 different concentrations of BA (6-Benzyl amino purine) and NAA (1-Naphthaleneacetic acid). The best callus formation (100%) was obtained from MS medium containing 0.5 mg/L BA+1.5 mg/L NAA; 1 mg/L BA+0.5 mg/L NAA; 1 mg/L BA+1 mg/L NAA; 0.5 mg/L BA; 1.5 mg/L BA+0.5 mg/L NAA; 1.5 mg/L BA+1.5 mg/L NAA; 2 mg/L BA or 2.5 mg/L BA+1.5 mg/L NAA. In shoot formation, 100% success was achieved in MS media containing 0.5 mg/L BA, 1 mg/L BA+0.5 mg/L NAA or 2 mg/L BA.

Özet

Turunçgillerin sahip olduğu tür çeşitliliği ve ekonomik değeri nedeniyle klasik ıslah çalışmaları yanında bitki biyoteknolojisi çalışmaları da ön plana çıkmaktadır. Bu çalışmada, Carrizo sitranjının in vıtro koşullarda çimlendirilen tohumlarından gelişen kök eksplantları kullanılarak kökten bitki rejenerasyonunun optimizasyonu hedeflenmiştir. Carrizo sitranjına ait kök eksplantları BA (6-Benzil amino purin) ve NAA (1- Naftalenasetik asit)’nın 21 farklı konsantrasyonunu içeren MS (Murashige ve Skoog, 1962) besin ortamında kültüre alınmışlardır. Çalışma sonucunda en iyi kallus oluşumu 0.5 mg/L BA+1.5 mg/L NAA; 1 mg/L BA+0.5 mg/L NAA; 1 mg/L BA+1 mg/L NAA; 0.5 mg/L BA; 1.5 mg/L BA+0.5 mg/L NAA; 1.5 mg/L BA+1.5 mg/L NAA; 2 mg/L BA veya 2.5 mg/L BA+1.5 mg/L NAA içeren MS ortamından elde edilmiştir (%100). Sürgün oluşumunda 0.5 mg/L BA, 1 mg/L BA+0.5 mg/L NAA veya 2 mg/L BA içeren MS ortamlarında %100 başarı elde edilmiştir.

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Cite This Article

İNCEOĞLU, Ü., DÖNMEZ, D., ŞİMŞEK, Ö., YEŞİLOĞLU, T., KAÇAR, Y. A. (2018). Optimization of Plant Regeneration from Root Explants of Carrizo citrange (Citrus sinensis Osb. X Poncirus trifoliata Raf. var “Carrizo”). Alatarım, Cilt: 17 - Sayı: 1, 18–26. https://ajindex.com/articles/141720

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