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Journals / Harran Üniversitesi Veteriner Fakültesi Dergisi / 2026 / Volume: 15 Issue: 1

Isolation, Antimicrobial Susceptibility Determination and Genotyping of Sphingomonas paucimobilis from Milk Samples of Cows with Mastitis

Mastitisli İneklerin Süt Örneklerinden Sphingomonas paucimobilis'in İzole Edilmesi, Antimikrobiyal Duyarlılığının Belirlenmesi ve Genotiplendirilmesi

Ayşe Öner(Aydın Adnan Menderes Üniversitesi Sağlık Bilimleri Enstitüsü Veterinerlik Mikrobiyolojisi Anabilim Dalı)0009-0005-4821-3747
Serap Savaşan(AYDIN ADNAN MENDERES ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, KLİNİK ÖNCESİ BİLİMLERİ BÖLÜMÜ, VETERİNERLİK MİKROBİYOLOJİSİ ANABİLİM DALI)0000-0002-9826-077X
Çağatay Nuhay(İzmir Bornova Araştırma Enstitüsü)0000-0002-1475-3041
Pages
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Publication Date
2026-03-27
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings indicate that S. paucimobilis should be considered among potential environmental mastitis pathogens and highlight the need for advanced diagnostic methods and effective antimicrobial stewardship to prevent resistance development.

📌 3 Key Takeaways

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Objective & Hypothesis:Mastitis is one of the most economically significant diseases affecting dairy cattle worldwide, with its subclinical form causing greater production losses than the clinical form.
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Key Findings & Data:A total of 100 milk samples were collected from cows with mastitis (50 clinical and 50 subclinical cases).
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Conclusion & Impact:The findings indicate that S. paucimobilis should be considered among potential environmental mastitis pathogens and highlight the need for advanced diagnostic methods and effective antimicrobial stewardship to prevent resistance development.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antibiotic Resistance · Genotype · Mastitis

Abstract

Mastitis is one of the most economically significant diseases affecting dairy cattle worldwide, with its subclinical form causing greater production losses than the clinical form. Sphingomonas paucimobilis , a Gram-negative, yellow-pigmented, aerobic bacillus, has recently been recognized as an emerging environmental pathogen in cases of mastitis. This study aimed to isolate S. paucimobilis from milk samples of cows with mastitis in the İzmir and Aydın regions, determine its antimicrobial susceptibility, and perform genotyping. A total of 100 milk samples were collected from cows with mastitis (50 clinical and 50 subclinical cases). Conventional bacteriological methods, VITEK® Compact 2, and PCR were used for identification. Eight (8%) S. Paucimobilis isolates were obtained, including six from subclinical mastitis samples and two from clinical mastitis samples. Antimicrobial susceptibility testing by the Kirby-Bauer disk diffusion method revealed high resistance rates to amoxicillin-clavulanic acid (87.5%), ampicillin-sulbactam (100%), and cefquinome (100%), while all isolates were susceptible to cefoperazone and gentamicin. Genotyping using the (GTG)₅-PCR method showed that all isolates had distinct patterns, with genetic similarity ranging from 32% to 98%. The findings indicate that S. paucimobilis should be considered among potential environmental mastitis pathogens and highlight the need for advanced diagnostic methods and effective antimicrobial stewardship to prevent resistance development.

Özet

Mastitis, dünya genelinde süt sığırlarını etkileyen en önemli ekonomik hastalıklardan biridir ve subklinik formu, klinik formuna göre daha büyük üretim kayıplarına yol açmaktadır. Gram-negatif, sarı pigmentli, aerob bir basillus olan Sphingomonas paucimobilis, son zamanlarda mastitis vakalarında ortaya çıkan çevresel bir patojen olarak tanımlanmıştır. Bu çalışmada, İzmir ve Aydın bölgelerindeki mastitisli ineklerden alınan süt örneklerinden S. paucimobilis izole edilmesi, antimikrobiyal duyarlılığının belirlenmesi ve genotiplendirme yapılması amaçlanmıştır. Sağlıklı ve mastitisli ineklerden toplam 100 süt örneği toplanmıştır. Tanımlama için konvansiyonel bakteriyolojik yöntemler, VITEK® Compact 2 sistemi ve PCR yöntemi kullanılmıştır. Sekiz (%8) S. paucimobilis izolatı elde edilmiştir. Kirby–Bauer disk difüzyon yöntemiyle yapılan antimikrobiyal duyarlılık testinde, amoksisilin–klavulanik aside (%87,5), ampisilin–sulbaktama (%100) ve sefkinoma (%100) karşı yüksek direnç oranları belirlenmiştir. Tüm izolatların sefopeperazon, gentamisin ve sefkinoma karşı duyarlı olduğu görülmüştür. (GTG)5-PCR yöntemiyle yapılan genotiplendirme sonucunda, tüm izolatların farklı tiplere sahip olduğu ve genetik benzerliğin %32 ile %98 arasında değiştiği tespit edilmiştir. Elde edilen bulgular, S. paucimobilis’in potansiyel çevresel mastitis etkenleri arasında değerlendirilmesi gerektiğini göstermekte olup, direnç gelişiminin önlenmesi amacıyla ileri tanı yöntemleri ve etkili antimikrobiyal yönetim stratejilerinin gerekliliğini ortaya koymaktadır.

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Cite This Article

Öner, A., Savaşan, S., Nuhay, Ç. (2026). Isolation, Antimicrobial Susceptibility Determination and Genotyping of Sphingomonas paucimobilis from Milk Samples of Cows with Mastitis. Harran Üniversitesi Veteriner Fakültesi Dergisi, Volume: 15 Issue: 1. https://doi.org/10.31196/huvfd.1791793

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