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Journals / Erciyes Üniversitesi Veteriner Fakültesi Dergisi / 2019 / Cilt: 16 Sayı: 3

Determination of Leucocyte CaSR Gene Expression Level and Plasma Calcium Level in Lactated Goats

Laktasyondaki Keçilerde Lökosit CaSR Gen Ekspresyon Seviyesi İle Plazma Kalsiyum Seviyesinin Belirlenmesi

Sevda Dalkıran
Akın Yakan0000-0002-9248-828X
Pages
184–189
Publication Date
2019-12-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, it has been found that regulation of calcium loss in blood which appears with milk of goats in lactation period is provided with CaSR gene.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Milk efficiency has a big importance in goat breeding.
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Key Findings & Data:Calcium exist in milk’s ingredient is significant for human health.
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Conclusion & Impact:In this study, it has been found that regulation of calcium loss in blood which appears with milk of goats in lactation period is provided with CaSR gene.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:CaSR · Damascus · gen ekspresyonu

Abstract

Milk efficiency has a big importance in goat breeding. Calcium exist in milk’s ingredient is significant for human health. Calcium takes part in many physological processes such as cell membrane permeability, oscillation of hormones, enzyme activity. The need for calcium also rises according to milk efficiency in lactation period for metabolism. Calcium is primarily organized by means of the Calcium Sensitive Receptor (CaSR) gene. In this study the relationship between the lactation and expression level of CaSR gene eith blood calcium level were evaluated in lactating Damascus goats. While CaSR expression gene was measured with RT-qPCR, plasma calcium level was measured with micro plasm atomic emission spectrophotometer (MP-AES). An important level of decline in calcium levels was found in the other months except for 1st and 3rd months. It has been found that the decline in the level of plasm calcium quantity was coherent with the decline of the expression level of CaSR. By this way it has been observed that metabolism lessens the CaSR expression by forming a protection reflex in order to prevent the falling of calcium lost with lactation. In this study, it has been found that regulation of calcium loss in blood which appears with milk of goats in lactation period is provided with CaSR gene.

Özet

Süt verimi keçi yetiştiriciliğinde büyük öneme sahiptir. Sütün içeriğinde bulunan kalsiyum insan sağlığı için önemlidir. Kalsiyum hücre membran geçirgenliği, hormonların salınımı, enzim aktivitesi gibi birçok fizyolojik olayda görev almaktadır. Metabolizma bakımından değerlendirildiğinde laktasyon döneminde süt verimine bağlı olarak kalsiyum ihtiyacı artmaktadır. Kalsiyum düzeyi ise önemli oranda Kalsiyuma Duyarlı Reseptör (CaSR) geni aracılığıyla düzenlenmektedir. Bu çalışmada laktasyondaki Damascus keçilerinde CaSR geni ekspresyon seviyesi ile kan kalsiyum seviyesi ölçülerek laktasyon ile aralarındaki ilişki araştırılmıştır. CaSR geni ekspresyon seviyesi RT-qPCR ile ölçülürken plazma kalsiyum seviyesi mikroplazma atomik emisyon spektrofotometresi (MP- AES) ile ölçülmüştür. Laktasyonun 1. ayına göre 3., 5. ve 7. aylarında CaSR genine ait ekspresyon seviyesinin önemli derecede azaldığı tespit edilmiştir. Laktasyonun 1. ve 3. aylarına göre diğer aylarda kalsiyum seviyelerinde önemli derecede azalma tespit edilmiştir. Plazma kalsiyum miktarındaki azalma CaSR geninin ekspresyon seviyesindeki azalma ile uyumlu olduğu tespit edilmiştir. Böylece laktasyon ile kaybolan kalsiyumun plazmadaki düşüşünü engellemek için metabolizmanın koruma refleksi oluşturarak CaSR ekspresyonunu azalttığı görülmüştür. Yapılan bu çalışmayla laktasyon dönemindeki keçilerde süt ile birlikte ortaya çıkan kalsiyum kaybının kandaki regülasyonunu CaSR geni ile sağlandığı tespit edilmiştir. 

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Cite This Article

Dalkıran, S., Yakan, A. (2019). Determination of Leucocyte CaSR Gene Expression Level and Plasma Calcium Level in Lactated Goats. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 16 Sayı: 3, 184–189. https://doi.org/10.32707/ercivet.648532

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