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Journals / Bozok Tıp Dergisi / 2026 / Cilt: 16 Sayı: 2

Effect of Rapamycin and 3-Methyladenine on Heat shock proteins in Cisplatin-induced Hepatotoxicity

Sisplatin kaynaklı hepatotoksisitede Rapamisin ve 3-Metiladenin'in Isı şoku proteinleri üzerindeki etkisi

Derya Karabulut(ERCIYES UNIVERSITY)
Nurhan Kuloğlu(NİĞDE ÖMER HALİSDEMİR ÜNİVERSİTESİ)0000-0002-1199-2784
Emel Öztürk(HARRAN UNIVERSITY)0000-0003-0756-0329
Meryem Sayan(ERCIYES UNIVERSITY)0000-0002-9068-1094
Emin Kaymak(BOZOK UNIVERSITY)0000-0002-3818-2693
Ali Tuğrul Akin(ISTINYE UNIVERSITY)0000-0002-1408-8571
Tuğçe Merve Öztürk(ERCIYES UNIVERSITY)0000-0002-4145-6907
Betül Yalçın(ADIYAMAN UNIVERSITY)0000-0003-1176-8843
Pages
215–223
Publication Date
2026-06-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, Rapamycin is more effective than 3-Methyladenine on HSPs in Cisplatin toxicity.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: This study aimed to investigate the effects of autophagy activator (rapamycin) and autophagy inhibitor (3-methyladenine) on heat shock proteins (HSP) in cisplatin-induced hepatotoxicity.
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Key Findings & Data:Materials and Methods: Wistar albino rats (28) in the study were divided into four equal groups.
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Conclusion & Impact:As a result, Rapamycin is more effective than 3-Methyladenine on HSPs in Cisplatin toxicity.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Isı şoku proteinleri · sisplatin · rapamisin

Abstract

Purpose: This study aimed to investigate the effects of autophagy activator (rapamycin) and autophagy inhibitor (3-methyladenine) on heat shock proteins (HSP) in cisplatin-induced hepatotoxicity. Materials and Methods: Wistar albino rats (28) in the study were divided into four equal groups. Control group, Cisplatin group; On day 7 of the experiment, a single dose of 8 mg/kg intraperitoneal (ip) cisplatin was administered, Rapamycin+Cisplatin (Rapamycin group); Starting from the 1st day of the experiment, rapamycin 2 mg/kg(ip) was administered once a day for 15 days, 8 mg/kg(ip) single-dose cisplatin was administered on the 7th day, 3-methyladenine+Cisplatin (3-Methyladenine group) 15 mg/kg( ip) was administered once a day for 15 days, 8 mg/kg(ip) single-dose of cisplatin was administered on the 7th day. Routine histological techniques were applied for liver tissues. Immunohistochemistry staining was performed to identify HSPs. TUNEL was performed to demonstrate apoptosis. Results: All HSPs in the cisplatin group were statistically increased compared to the control. Rapamycin resulted in decreased expression of all HSPs, which was increased by cis. 3-Methyladenine was less effective in HSPs compared to Rapamycin. TUNEL-positive cells increased statistically in the Cisplatin group. Rapamycin was similar to the control group. TUNEL-positive cells did not change in the 3-methyladenine group. Conclusion: We think that Rapamycin reduces both HSPs and related cell death by contributing to the reduction of Cisplatin-induced oxidative stress in the tissue. Inhibition of autophagy with 3-Methyladenine in tissue causes increased expression of Cisplatin-induced HSPs. As a result, Rapamycin is more effective than 3-Methyladenine on HSPs in Cisplatin toxicity.

Özet

Amaç: Bu çalışmanın amacı, otofaji aktivatörü (rapamisin) ve otofaji inhibitörünün (3-metiladenin) sisplatin kaynaklı hepatotoksisitede ısı şoku proteinleri (HSP) üzerindeki etkilerini araştırmaktı. Gereç ve Yöntem: Çalışmadaki Wistar albino sıçanları (28) dört eşit gruba ayrıldı. Kontrol grubu, Sisplatin grubu; Deneyin 7. gününde, tek doz 8 mg/kg intraperitoneal (ip) sisplatin uygulandı, Rapamisin+Sisplatin (Rapamisin grubu); Deneyin 1. gününden itibaren, rapamisin 2 mg/kg(ip) günde bir kez 15 gün boyunca uygulandı, 8 mg/kg(ip) tek doz sisplatin 7. günde uygulandı, 3-metiladenin+Sisplatin (3-Metiladenin grubu) 15 mg/kg(ip) günde bir kez 15 gün boyunca uygulandı, 8 mg/kg(ip) tek doz sisplatin 7. günde uygulandı. Karaciğer dokuları için rutin histolojik teknikler uygulandı. HSP'leri tanımlamak için immünohistokimya boyama yapıldı. Apoptozu göstermek için TUNEL yapıldı. Bulgular: Sisplatin grubundaki tüm HSP'ler kontrole kıyasla istatistiksel olarak arttı. Rapamisin, cis tarafından artırılan tüm HSP'lerin ekspresyonunun azalmasına neden oldu. 3-Metiladenin, Rapamisin ile karşılaştırıldığında HSP'lerde daha az etkiliydi. TUNEL pozitif hücreler Cisplatin grubunda istatistiksel olarak arttı. Rapamisin kontrol grubuna benzerdi. TUNEL pozitif hücreler 3-metiladenin grubunda değişmedi. Sonuç: Rapamisin'in dokuda Cisplatin kaynaklı oksidatif stresin azaltılmasına katkıda bulunarak hem HSP'leri hem de ilgili hücre ölümünü azalttığını düşünüyoruz. Dokuda 3-Metiladenin ile otofaji inhibisyonu Cisplatin kaynaklı HSP'lerin ekspresyonunun artmasına neden olur. Sonuç olarak Rapamisin, Cisplatin toksisitesinde HSP'ler üzerinde 3-Metiladenin'den daha etkilidir.

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Cite This Article

Karabulut, D., Kuloğlu, N., Öztürk, E., Sayan, M., Kaymak, E., Akin, A. T., Öztürk, T. M., Yalçın, B. (2026). Effect of Rapamycin and 3-Methyladenine on Heat shock proteins in Cisplatin-induced Hepatotoxicity. Bozok Tıp Dergisi, Cilt: 16 Sayı: 2, 215–223. https://doi.org/10.16919/bozoktip.1819527

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