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Journals / Black Sea Journal of Agriculture / 2026 / Cilt: 8 Sayı: 3

Comparison of Inoculation Methods for Screening Tomato, Pepper, and Aubergine Plants Inoculated with Neoscytalidium Novaehollandiae

Hümeyra Ayvacı(Harran Üniversitesi)0000-0002-5620-4147
Fatih Durmuş(HARRAN ÜNİVERSİTESİ)0000-0001-8149-0301
Mehmet Güldür(HARRAN ÜNİVERSİTESİ)0000-0002-3374-5602
Murat Dikilitas(HARRAN ÜNİVERSİTESİ)0000-0002-7399-4750
Pages
385–391
Publication Date
2025-05-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that the cut-stem and injection methods were effective on tomato, pepper, and aubergine plants while the spray inoculation method was not found effective.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Neoscytalidium novaehollandiae is a highly destructive fungus that causes serious yield losses in economically important crop plants.
📊
Key Findings & Data:A reliable and rapid inoculation method for Neoscytalidium novaehollandiae was evaluated in eggplant, tomato and pepper seedlings under greenhouse conditions.
💡
Conclusion & Impact:The results showed that the cut-stem and injection methods were effective on tomato, pepper, and aubergine plants while the spray inoculation method was not found effective.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Neoscytalidium novaehollandiae · Grafting techniques · Cut-stem

Abstract

Neoscytalidium novaehollandiae is a highly destructive fungus that causes serious yield losses in economically important crop plants. A reliable and rapid inoculation method for Neoscytalidium novaehollandiae was evaluated in eggplant, tomato and pepper seedlings under greenhouse conditions. Three different inoculation methods namely, cut-stem, spray, and injection were evaluated on tomato, pepper, and aubergine seedlings. The results showed that the cut-stem and injection methods were effective on tomato, pepper, and aubergine plants while the spray inoculation method was not found effective. Especially, the cut-stem inoculation method appears to be the most appropriate method for pathogenicity and screening of seedlings for N. novaehollandiae pathogenicity.

Özet

Neoscytalidium novaehollandiae is a highly destructive fungus that causes serious yield losses in economically important crop plants. A reliable and rapid inoculation method for Neoscytalidium novaehollandiae was evaluated in eggplant, tomato and pepper seedlings under greenhouse conditions. Three different inoculation methods namely, cut-stem, spray, and injection were evaluated on tomato, pepper, and aubergine seedlings. The results showed that the cut-stem and injection methods were effective on tomato, pepper, and aubergine plants while the spray inoculation method was not found effective. Especially, the cut-stem inoculation method appears to be the most appropriate method for pathogenicity and screening of seedlings for N. novaehollandiae pathogenicity.

🔖

Cite This Article

Ayvacı, H., Durmuş, F., Güldür, M., Dikilitas, M. (2025). Comparison of Inoculation Methods for Screening Tomato, Pepper, and Aubergine Plants Inoculated with Neoscytalidium Novaehollandiae. Black Sea Journal of Agriculture, Cilt: 8 Sayı: 3, 385–391. https://doi.org/10.47115/bsagriculture.1649429

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References (42)

  1. Agrios GN, 2005. Plant Pathology 5th Edition. ElSevier Academic Press, Burlington, MA, USA, pp: 922.
  2. Babalola FA, Egwari LO, 2017. Erwinia rots and presence of pathogenic bacteria in symptomless fruits and vegetables. Covenant J Phys Life Sci, 5(1): 1-16.
  3. Bakhshizadeh M, Hashemian HR, Najafzadeh MJ, Dolatabadi S, Zarrinfar H, 2014. First report of rhinosinusitis caused by Neoscytalidium dimidiatum in Iran. Med Microbiol, 63(7): 1017-1019.
  4. Chaturvedi S, Pandey R, 2021. Bioinoculant with vermicompost augments essential oil constituents and antioxidants in Mentha arvensis L. J Plant Growth Regul, 40: 1284-1297.
  5. Chen W, Lee MK, Jefcoate C, Kim SC, Chen F, Yu JH, 2014. Fungal cytochrome p450 monooxygenases: their distribution, structure, functions, family expansion, and evolutionary origin. Genome Biol Evol, 6: 1620-1634.
  6. Chethana KWT, Li XH, Zhang W, Hyde KD, Yan JY, 2016. Trail decryption of molecular research on Botryosphaeriaceae in woody plants. Phytopathol Mediterr, 55: 147–71.
  7. Crous P, Slippers B, Michael J, Wingfield MJ, Walter MF, Philips A, Johannes ZG, 2006. Phylogenetic lineages in the Botryosphaeriaceae. Stud Mycol, 5: 235-253.
  8. Ćuk N, Cvejić S, Mladenov V, Miladinović D, Babec B, Jocić S, Dedić B, 2022. Introducing a cut-stem inoculation method for fast evaluation of sunflower resistance to Macrophomina phaseolina. Phytoparasitica, 50(4): 775-788.
  9. Da Silva RT, Guimaraes DA, Camargo ZP, Rodrigues AM, Maceira JP, Bernardes Engemann AR, Orofinocosta R, 2016. Cutaneous murine model of infection caused by Neoscytalidium dimidiatum: a preliminary study of an emerging human pathogen. Med Mycol, 54: 890-898.
  10. Dervis S, Özer G, Türkölmez Ş, 2020a. First report of Neoscytalidium dimidiatum causing tuber rot of potato in Türkiye. J Plant Pathol, 102(4): 1295-1296.
  11. Dervis S, Özer G, Türkölmez Ş, 2020b. First report of Neoscytalidium novaehollandiae causing stem blight on tomato in Türkiye. J Plant Pathol, 102(4): 1339-1340.
  12. Dikilitaş M, 2003. Effect of salinity and its interactions with Verticillium albo-atrum on the disease development in tomato (Lycopersicon esculentum Mill) and lucerne (Medicago sativa L and M. media) plants. Swansea University (United Kingdom), London, UK, pp: 47.
  13. Elad Y, Pertot I, 2014. Climate change impacts on plant pathogens and plant diseases. J Crop Improv, 28(1): 99-139.
  14. Félix C, Duarte AS, Vitorino R, Guerreiro ACL, Domingues P, Correia ACM, Alves A, Esteves AC, 2016. Temperature modulates the secretome of the phytopathogenic fungus Lasiodiplodia theobromae. Front Plant Sci, 7: 1-12.
  15. Fernando WD, Nakkeeran S, Zhang Y, 2004. Ecofriendly methods in combating Sclerotinia sclerotiorum (Lib.) de Bary. Recent Res Dev Environ Biol, 1: 329-347.
  16. Garcia JF, Lawrence DP, Morales-Cruz A, Travadon R, Minio A, Hernandez-Martinez R, Rolshausen RE, Baumgartner K, Cantu D, 2021. Phylogenomics of plant-associated Botryosphaeriaceae species. Front Microbiol, 12: 652802.
  17. Güney İG, Bozoğlu T, Özer G, Türkölmez Ş, Dervis S, 2022a. First report of Neoscytalidium dimidiatum associated with dieback and canker of common fig (Ficus carica L.) in Türkiye. J Plant Dis Prot, pp: 1-5.
  18. Güney İG, Özer G, Türkölmez Ş, Dervis S, 2022b. Canker and leaf scorch on olive (Olea europaea L.) caused by Neoscytalidium dimidiatum in Türkiye. Crop Prot, 157: 105985.
  19. Höfte M, Vos P, 2006. Plant pathogenic Pseudomonas species. Plant-associated bacteria, pp: 507-533.
  20. Hua L, Yong C, Zhanquan Z, Boqiang L, Guozheng Q, Shiping T, 2018. Pathogenic mechanisms and control strategies of Botrytis cinerea causing post-harvest decay in fruits and vegetables. Food Qual Saf, 2(3): 111-119.
  21. Jami F, Slippers B, Wingfield MJ, Gryzenhout M, 2014. Botryosphaeriaceae species overlap on four unrelated, native South African hosts. Fungal Biol, 118(2): 168-179.
  22. Jamieson MA, Trowbridge AM, Raffa KF, Lindroth RL, 2012. Consequences of climate warming and altered precipitation patterns for plant-insect and multitrophic interactions. Plant Physiol, 160(4): 1719-1727.
  23. Jordaan AJ, Mlenga DH, Mandebvu B, 2019. Monitoring droughts in Eswatini: A spatiotemporal variability analysis using the Standard Precipitation Index. Jàmbá, 11(1): 1-11.
  24. Kee YJ, Suhaimi NN, Zakaria L, Mohd MH, 2017. Characterisation of Neoscytalidium dimidiatum causing leaf blight on Sansevieria trifasciata in Malaysia. Australas Plant Dis Notes, 12: 60.
  25. Khamari B, Hasmi SK, 2019. Biointensive management of Macrophomina phaseolina, inciting stem and root rot of sesame. Curr Res Innov Plant Pathol, 7: 1-26.
  26. Kılınç B, 2021. Şanlıurfa ilinde Antepfıstığı (Pistacia vera L.) ağaçlarında Neoscytalidium novaehollandiae’nın bulaşıklık oranının belirlenmesi, morfolojik ve moleküler karakterizasyonu ve in vitro fungusit duyarlılığı. Yüksek Lisans tezi, Harran Üniversitesi, Fen Bilimleri Enstitüsü, Şanlıurfa, pp: 36.
  27. Machouart M, Menir P, Helenon R, Quist D, Desbois N, 2013. Scytalidium and scytalidiosis: what's new in 2012. J Mycol Méd, 23(1): 40-46.
  28. Mengistu A, Ray JD, Smith GD, Paris RL, 2007. Charcoal rot disease assessment of soybean genotypes using colony-forming units index. Crop Sci, 47: 2453-2461.
  29. Paolinelli-Alfonso M, Villalobos-Escobedo JM, Rolshausen P, Herrera-Estrella A, Galindo-Sánchez C, López-Hernández JF, Hernandez-Martínez R, 2016. Global transcriptional analysis suggests Lasiodiplodia theobromae pathogenicity factors involved in modulation of grapevine defensive response. BMC Genomics, 17: 615.
  30. Pavlic D, Wingfield MJ, Barber P, Slippers B, Hardy GESJ, Burgess TI, 2008. Seven new species of the Botryosphaeriaceae from baobab and other native trees in Western Australia. Mycologia, 100(6): 851-866.
  31. Talapov T, 2020. Determination of sunflower (Helianthus annuus) ve soybean (Glycine max) variety reactions to Macrophomina phaseolina and characterization of the agent. Yüksek Lisans tezi, Gaziantep Üniversitesi, Fen Bilimleri Enstitüsü, Gaziantep, pp: 42.
  32. Talapov T, Yüceer S, Dedecan O, Demirel O, Can C, 2021. Comparison of Macrophomina phaseolina inoculation techniques for screening sunflower and soybean germplasm in a controlled environment. Can J Plant Pathol, 43(6): 859-870.
  33. Todorović B, Potočnik I, Rekanović E, Stepanović M, Kostić M, Ristić M, Milijašević-Marčić S, 2016. Toxicity of twenty-two plant essential oils against pathogenic bacteria of vegetables and mushrooms. J Environ Sci Health B, 51(12): 832-839.
  34. TÜİK, 2024. ‘Bitkisel Üretim İstatistikleri’ 02.01.2024. https://biruni.tuik.gov.tr/medas/?kn=92&locale=tr (accessed date: August 6, 2024).
  35. Türkölmez Ş, Dervis S, Çiftçi O, Dikilitaş M, 2019b. First report of Neoscytalidium dimidiatum causing shoot and needle blight of pines (Pinus spp.) in Türkiye. Plant Dis, 103(11): 2960.
  36. Türkölmez Ş, Dervis S, Çiftçi O, Serçe ÇU, Dikilitaş M, 2019a. New disease caused by Neoscytalidium dimidiatum devastates tomatoes (Solanum lycopersicum) in Türkiye. Crop Prot, 118: 21-30.
  37. Twizeyimana M, Hill CB, Pawlowski M, Paul C, Hartman GL, 2012. A cut-stem inoculation technique to evaluate soybean for resistance to Macrophomina phaseolina. Plant Dis, 96(8): 1210–1215.
  38. Wolters PJ, Faino L, Van Den Bosch TB, Evenhuis B, Visser RG, Seidl MF, Vleeshouwers VG, 2018. Gapless genome assembly of the potato and tomato early blight pathogen Alternaria solani. Mol Plant Microbe Interact, 31(7): 692-694.
  39. Yan JY, Zhao WS, Chen Z, Xing QK, Zhang W, Chethana WKT, Xue MF, Xu JP, Phillips AJL, Wang Y, Liu JH, Liu M, Zhou Y, Jayawardena RS, Manawasinghe IS, Huang JB, Qiao GH, Fu CY, Guo FF, Dissanayake AJ, Peng YL, Hyde KD, Li XH, 2018. Comparative genome and transcriptome analyses reveal adaptations to opportunistic infections in woody plant degrading pathogens of Botryosphaeriaceae. DNA Res, 25(1): 87–102.
  40. Yardımcı N, Çulal-Kılıç H, 2009. Tomato spotted wilt virus in vegetable growing areas in the west Mediterranean region of Türkiye. Afr J Biotechnol, 8(18): 4539-4541.
  41. Zhao Y, Li P, Huang K, Wang Y, Hu H, Sun Y, 2013. Control of postharvest soft rot caused by Erwinia carotovora of vegetables by a strain of Bacillus amyloliquefaciens and its potential modes of action. World J Microbiol Biotechnol, 29(3): 411-420.
  42. Zitikaitė I, Urbanavičienė L, 2010. Detection of natural infection by Cucumber mosaic virus in vegetable crops. Biologija, 56: 1-4.