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Journals / Black Sea Journal of Agriculture / 2026 / Cilt: 8 Sayı: 4

Genome-Wide in silico Analysis and Identification of Bos taurus SOX Gene Family

Emre İlhan(ERZURUM TECHNICAL UNIVERSITY)0000-0002-8404-7900
Yusuf Ziya Güzey(Hatay Mustafa Kemal Üniversitesi)0000-0002-4900-6038
Pages
563–569
Publication Date
2025-07-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings of this research offer a valuable resource for gaining a deeper insight into the molecular foundation of the SOX gene family in Bos taurus.

📌 3 Key Takeaways

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Objective & Hypothesis:The SOX (SRY-box transcription factors) gene family is defined by the presence of the HMG-box (High-mobility group) domain, which was first discovered in the SRY (sex-determining region on the Y chromosome) gene located on the Y chromosome, and plays a vital role in the initiation of male sex determination.
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Key Findings & Data:A total of 15 SOX proteins were identified in the Bos taurus genome.
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Conclusion & Impact:The findings of this research offer a valuable resource for gaining a deeper insight into the molecular foundation of the SOX gene family in Bos taurus.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Bos taurus · SOX gene · in silico

Abstract

The SOX (SRY-box transcription factors) gene family is defined by the presence of the HMG-box (High-mobility group) domain, which was first discovered in the SRY (sex-determining region on the Y chromosome) gene located on the Y chromosome, and plays a vital role in the initiation of male sex determination. The purpose of this study was to genome-wide identify and characterize SOX gene family members in silico in Bos taurus. A total of 15 SOX proteins were identified in the Bos taurus genome. These proteins weigh between 26.49 (Bt-SOX-14) and 93.35 (Bt-SOX-6) kDa and have theoretical isoelectric points ranging from 4.95-9.85. As a result of phylogenetic analyses, Bt-SOX proteins and SOX proteins of Mus musculus and Homo sapiens species were clustered in 7 main groups. Segmental duplication was also detected between Bt-SOX-14/Bt-SOX-21, Bt-SOX-5/Bt-SOX-6, Bt-SOX-9/Bt-SOX-10 and Bt-SOX-4/Bt-SOX-11 gene pairs. On the other hand, according to in silico gene expression, Bt-SOX genes exhibited different expression profiles in different tissues and were found to have the highest expression in brain and testicular tissues. This indicates that the majority of Bt-SOX genes have tissue-specific expression. The findings of this research offer a valuable resource for gaining a deeper insight into the molecular foundation of the SOX gene family in Bos taurus.

Özet

The SOX (SRY-box transcription factors) gene family is defined by the presence of the HMG-box (High-mobility group) domain, which was first discovered in the SRY (sex-determining region on the Y chromosome) gene located on the Y chromosome, and plays a vital role in the initiation of male sex determination. The purpose of this study was to genome-wide identify and characterize SOX gene family members in silico in Bos taurus. A total of 15 SOX proteins were identified in the Bos taurus genome. These proteins weigh between 26.49 (Bt-SOX-14) and 93.35 (Bt-SOX-6) kDa and have theoretical isoelectric points ranging from 4.95-9.85. As a result of phylogenetic analyses, Bt-SOX proteins and SOX proteins of Mus musculus and Homo sapiens species were clustered in 7 main groups. Segmental duplication was also detected between Bt-SOX-14/Bt-SOX-21, Bt-SOX-5/Bt-SOX-6, Bt-SOX-9/Bt-SOX-10 and Bt-SOX-4/Bt-SOX-11 gene pairs. On the other hand, according to in silico gene expression, Bt-SOX genes exhibited different expression profiles in different tissues and were found to have the highest expression in brain and testicular tissues. This indicates that the majority of Bt-SOX genes have tissue-specific expression. The findings of this research offer a valuable resource for gaining a deeper insight into the molecular foundation of the SOX gene family in Bos taurus.

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Cite This Article

İlhan, E., Güzey, Y. Z. (2025). Genome-Wide in silico Analysis and Identification of Bos taurus SOX Gene Family. Black Sea Journal of Agriculture, Cilt: 8 Sayı: 4, 563–569. https://doi.org/10.47115/bsagriculture.1719387

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