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Journals / Black Sea Journal of Agriculture / 2026 / Cilt: 9 Sayı: 1

The Effect of Ascorbic Acid Applications on Biochemical Parameters in Medicinal Sage Grown under Salt Stress

Ezelhan Şelem(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ MURADİYE MESLEK YÜKSEKOKULU PARK VE BAHÇE BİTKİLERİ BÖLÜMÜ)0000-0003-4227-5013
Lütfi Nohutçu(Van Yüzüncü Yıl Ünversitesi, Ziraat Fakültesi,Tarla Bitkileri Bölümü, Tarla Bitkileri Ana Bilim Dalı)0000-0003-2250-2645
Rüveyde Tunçtürk(Van Yüzüncü Yıl Ünversitesi, Ziraat Fakültesi,Tarla Bitkileri Bölümü, Tarla Bitkileri Ana Bilim Dalı)0000-0002-3759-8232
Murat Tunçtürk(Van Yüzüncü Yıl Ünversitesi, Ziraat Fakültesi,Tarla Bitkileri Bölümü, Tarla Bitkileri Ana Bilim Dalı)0000-0002-7995-0599
Pages
89–99
Publication Date
2026-01-13
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings indicate that both AA doses and salt stress levels, either alone or in combination, cause significant changes in the plant's defense mechanisms and metabolic regulation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the effects of different doses of ascorbic acid (AA) applications on the physiological and biochemical responses of medicinal sage under salt stress were evaluated in a multifaceted manner.
📊
Key Findings & Data:In the study, ascorbic acid was applied at different concentrations (0 ppm, 100 ppm, 200 ppm, and 300 ppm), and the plants were exposed to salt stress at different doses (0 mM, 100 mM, and 200 mM NaCl).
💡
Conclusion & Impact:The findings indicate that both AA doses and salt stress levels, either alone or in combination, cause significant changes in the plant's defense mechanisms and metabolic regulation.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:NBI · Anthocyanin · Antioxidant

Abstract

In this study, the effects of different doses of ascorbic acid (AA) applications on the physiological and biochemical responses of medicinal sage under salt stress were evaluated in a multifaceted manner. In the study, ascorbic acid was applied at different concentrations (0 ppm, 100 ppm, 200 ppm, and 300 ppm), and the plants were exposed to salt stress at different doses (0 mM, 100 mM, and 200 mM NaCl). In the study, Dualex parameters (NBI (Nitrogen Balance Index), chlorophyll, flavonoids, anthocyanins) and some biochemical components (total antioxidant activity, total flavonoids, total phenolics, and ascorbic acid content) were determined. Based on the obtained data, the total ascorbic acid content was found to be statistically insignificant, while all other parameters were found to be significant. The lowest and highest values for biochemical parameters were 68.78-176. 59 µmol TE g-1, total flavonoids 6.48-13.77 mg QE 100g-1, total phenolic content 174.00-237.39 mg GAE g-1, and ascorbic acid 19.69-24.92 mg 100g-1. In Dualex measurements, the lowest and highest values were found to be 37.50–74.70 dx for NBI, 25.83–49.40 dx for chlorophyll, 0.56–0.87 dx for flavonoids, and 0.013–0.080 dx for anthocyanins. The findings indicate that both AA doses and salt stress levels, either alone or in combination, cause significant changes in the plant's defense mechanisms and metabolic regulation.

Özet

In this study, the effects of different doses of ascorbic acid (AA) applications on the physiological and biochemical responses of medicinal sage under salt stress were evaluated in a multifaceted manner. In the study, ascorbic acid was applied at different concentrations (0 ppm, 100 ppm, 200 ppm, and 300 ppm), and the plants were exposed to salt stress at different doses (0 mM, 100 mM, and 200 mM NaCl). In the study, Dualex parameters (NBI (Nitrogen Balance Index), chlorophyll, flavonoids, anthocyanins) and some biochemical components (total antioxidant activity, total flavonoids, total phenolics, and ascorbic acid content) were determined. Based on the obtained data, the total ascorbic acid content was found to be statistically insignificant, while all other parameters were found to be significant. The lowest and highest values for biochemical parameters were 68.78-176. 59 µmol TE g-1, total flavonoids 6.48-13.77 mg QE 100g-1, total phenolic content 174.00-237.39 mg GAE g-1, and ascorbic acid 19.69-24.92 mg 100g-1. In Dualex measurements, the lowest and highest values were found to be 37.50–74.70 dx for NBI, 25.83–49.40 dx for chlorophyll, 0.56–0.87 dx for flavonoids, and 0.013–0.080 dx for anthocyanins. The findings indicate that both AA doses and salt stress levels, either alone or in combination, cause significant changes in the plant's defense mechanisms and metabolic regulation.

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Cite This Article

Şelem, E., Nohutçu, L., Tunçtürk, R., Tunçtürk, M. (2026). The Effect of Ascorbic Acid Applications on Biochemical Parameters in Medicinal Sage Grown under Salt Stress. Black Sea Journal of Agriculture, Cilt: 9 Sayı: 1, 89–99. https://doi.org/10.47115/bsagriculture.1811669

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