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Journals / Black Sea Journal of Agriculture / 2026 / Cilt: 9 Sayı: 5

Synthetic Seed Production and Short-Term Conservation of Hawthorn (Crataegus monogyna) Using Nodal Segments

Metin Koçak(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, TARIMSAL BİYOTEKNOLOJİ BÖLÜMÜ)0000-0002-8109-5245
Pages
740–747
Publication Date
2026-09-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Results indicated that storage period had no significant effect on germination rates, and the synthetic seeds retained their viability for up to 3 months.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Hawthorn (Crataegus monogyna) is an important fruit species with high nutritional and pharmacological value.
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Key Findings & Data:Seed dormancy, low germination rates and variations in generative propagation make propagation of the species and conservation of its genetic resources difficult.
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Conclusion & Impact:Results indicated that storage period had no significant effect on germination rates, and the synthetic seeds retained their viability for up to 3 months.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Crataegus monogyna · Synthetic seed · Storage duration

Abstract

Hawthorn (Crataegus monogyna) is an important fruit species with high nutritional and pharmacological value. Seed dormancy, low germination rates and variations in generative propagation make propagation of the species and conservation of its genetic resources difficult. In the present study, synthetic seeds were produced from nodal segments of C. monogyna, and the effect of different storage periods and Indole-3-acetic acid (IAA) on germination, micropropagation and rooting performance of the synthetic seeds was examined. Synthetic seeds were produced by 3% sodium alginate and hardened in a 100 mM CaCl2 solution. The synthetic seeds obtained were cultured on hormone-free Woody Plant Media (HF-WPM) and on WPM supplemented with 2 mg/L IAA, either immediately or after storage for 1, 2 and 3 months in the dark at 4°C. Results indicated that storage period had no significant effect on germination rates, and the synthetic seeds retained their viability for up to 3 months. On the other hand, micropropagation and rooting were significantly influenced by storage duration. The highest micropropagation rates were observed after 2 and 3 months of storage, but rooting was observed only in synthetic seeds stored for 3 months. The addition of 2 mg/L IAA to the medium did not increase germination but induced micropropagation. The results indicated that the synthetic seed technology is an effective method for short-term conservation and in vitro propagation of hawthorn genetic resources.

Özet

Hawthorn (Crataegus monogyna) is an important fruit species with high nutritional and pharmacological value. Seed dormancy, low germination rates and variations in generative propagation make propagation of the species and conservation of its genetic resources difficult. In the present study, synthetic seeds were produced from nodal segments of C. monogyna, and the effect of different storage periods and Indole-3-acetic acid (IAA) on germination, micropropagation and rooting performance of the synthetic seeds was examined. Synthetic seeds were produced by 3% sodium alginate and hardened in a 100 mM CaCl2 solution. The synthetic seeds obtained were cultured on hormone-free Woody Plant Media (HF-WPM) and on WPM supplemented with 2 mg/L IAA, either immediately or after storage for 1, 2 and 3 months in the dark at 4°C. Results indicated that storage period had no significant effect on germination rates, and the synthetic seeds retained their viability for up to 3 months. On the other hand, micropropagation and rooting were significantly influenced by storage duration. The highest micropropagation rates were observed after 2 and 3 months of storage, but rooting was observed only in synthetic seeds stored for 3 months. The addition of 2 mg/L IAA to the medium did not increase germination but induced micropropagation. The results indicated that the synthetic seed technology is an effective method for short-term conservation and in vitro propagation of hawthorn genetic resources.

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Cite This Article

Koçak, M. (2026). Synthetic Seed Production and Short-Term Conservation of Hawthorn (Crataegus monogyna) Using Nodal Segments. Black Sea Journal of Agriculture, Cilt: 9 Sayı: 5, 740–747. https://doi.org/10.47115/bsagriculture.1975847

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References (52)

  1. Abbas, M. K., Mahood, H. E., & Alhasan, A. S. (2022). Production of synthetic seeds in vegetable crops: A review. In IOP Conference Series: Earth and Environmental Science. https://doi.org/10.1088/1755-1315/1060/1/012099
  2. Adhikari, S., Bandyopadhyay, T. K., & Ghosh, P. (2014). Assessment of genetic stability of Cucumis sativus L. regenerated from encapsulated shoot tips. Scientia Horticulturae, 170, 115-122. https://doi.org/10.1016/j.scienta.2014.01.015
  3. Ahmad, A., Ansari, F. A., Anis, M., & Khan, A. S. (2023). Micropropagation of Pterocarpus marsupium Roxb. through synthetic seeds and its novel antibiofilm activities against ESKAPE pathogens. Industrial Crops and Products, 198, 116681. https://doi.org/10.1016/j.indcrop.2023.116681
  4. Aierken, A., Buchholz, T., Chen, C., Zhang, X., & Melzig, M. F. (2017). Hypoglycemic effect of hawthorn in type II diabetes mellitus rat model. Journal of the Science of Food and Agriculture, 97(13), 4557–4561. https://doi.org/10.1002/jsfa.8323
  5. Ara, H., Jaiswal, U., & Jaiswal, V. (2000). Synthetic seed: Prospects and limitation. Current Science, 78, 1438–1444.
  6. Bahorun, T., Aumjaud, E., Ramphul, H., Rycha, M,. Luximon-Ramma, A., Trotin, F., & Aruoma, O. (2003). Phenolic constituents and antioxidant capacities of Crataegus monogyna (hawthorn) callus extracts. Food/Nahrung, 47(3):191–198. https://doi.org/10.1002/food.200390045
  7. Bekheet, S. A. (2006). A synthetic seed method through encapsulation of in vitro proliferated bulblets of garlic (Allium sativum L.). Arab Journal of Biotechnology, 9(3), 415-426.
  8. Chand, S., & Singh, A. K. (2004). Plant regeneration from encapsulated nodal segments of Dalbergia sissoo Roxb., a timber-yielding leguminous tree species. Journal of Plant Physiology, 161(2), 237-243. https://doi.org/10.1078/0176-1617-01036
  9. Chang, Q., Zuo, Z., Chow, M. S., & Ho, W. K. (2006). Effect of storage temperature on phenolics stability in hawthorn (Crataegus pinnatifida var. major) fruits and a hawthorn drink. Food Chemistry, 98, 426–430. https://doi.org/10.1016/j.foodchem.2005.06.015
  10. Cheruvathur, M. K., Najeeb, N., & Tomas, T. D. (2013). In vitro propagation and conservation of Indian sarsaparilla, Hemidesmus indicus L. R. Br. through somatic embryogenesis and synthetic seed production, Acta Physiologiae Plantarum, 35(3), 771–779. https://doi.org/10.1007/s11738-012-1117-5
  11. Daud, M., Taha, M. Z., & Hasbullah, A.Z. (2008). Artificial seed production from encapsulated micro shoots of Sainpaulia ionantha Wendl. (African Violet). Journal of Applied Sciences, 8, 4662–4667. https://doi.org/10.3923/jas.2008.4662.4667
  12. Dönmez, D. (2022). Regeneration of plants from alginate-encapsulated shoot tips of myrtle (Myrtus communis L.). Erwerbs-Obstbau, 64(2), 307-314. https://doi.org/10.1007/s10341-021-00632-z
  13. Erkek, B., Yaman, M., Sümbül, A., Demirel, S., Demirel, F., Coşkun, Ö. F., Say. A., Eren B., Aydin, A & Eroglu, A. (2025). Natural diversity of Crataegus monogyna Jacq. in Northeastern Türkiye encompassing morphological, biochemical, and molecular features. Horticulturae, 11(3), 238. https://doi.org/10.3390/horticulturae11030238
  14. Faisal, M., Alatar, A. A., Ahmad, N., Anis, M., & Hegazy, A. K. (2012). Assessment of genetic fidelity in Rauvolfia serpentina plantlets grown from synthetic (encapsulated) seeds following in vitro storage at 4 C. Molecules, 17(5), 5050-5061. https://doi.org/10.3390/molecules17055050
  15. Ganapathi, T. R., Suprasanna, P., Bapat, V. A., & Rao, P. S., (1992). Propagation of banana through encapsulated shoot tips. Plant Cell Reports, 11(11), 571–575. https://doi.org/10.1007/bf00233095
  16. Gantait, S., Kundu, S., Yeasmin, L., & Ali, M. N. (2017). Impact of differential levels of sodium alginate, calcium chloride and basal media on germination frequency of genetically true artificial seeds of Rauvolfia serpentina (L.) Benth. ex Kurz. Journal of Applied Research on Medicinal and Aromatic Plants, 4, 75-81. https://doi.org/10.1016/j.jarmap.2017.01.005
  17. Genç, S., & Soysal, M. İ. (2018). Parametric and nonparametric post hoc tests. Black Sea Journal of Engineering and Science, 1(1), 18-27. https://izlik.org/JA73XC42GW
  18. Ghosh, P. K., Ghorai, S., Bhunia, R., Samanta, A., Bose, S., Mondal, M. S. A., Roy S., & Hazra, S. (2026). Synthetic seed technology and in vitro conservation of vegetable germplasm: a review. Vegetos, 39(2), 489-508. https://doi.org/10.1007/s42535-025-01321-y
  19. Haque, S. M., & Ghosh, B. (2017). Regeneration of cytologically stable plants through dedifferentiation, redifferentiation, and artificial seeds in Spathoglottis plicata Blume (Orchidaceae). Horticultural Plant Journal, 3(5), 199–208. https://doi.org/10.1016/j.hpj.2017.10.002
  20. Hatzilazarou, S., Kostas, S., Joachim, M., & Economou, A. (2020). Regeneration of Viburnum dentatum L. from alginate-encapsulated shoot explants after short-term cold storage and assessment of genetic stability using ISSR analysis. Agronomy, 10(11), 1660. https://doi.org/10.3390/agronomy10111660
  21. Hatzilazarou, S., Kostas, S., Nendou, T., & Economou, A. (2021). Conservation, regeneration and genetic stability of regenerants from alginate-encapsulated shoot explants of Gardenia jasminoides Ellis. Polymers, 13(10), 1666. https://doi.org/10.3390/polym13101666
  22. Hung, C. D., & Trueman, S. J. (2012). Preservation of encapsulated shoot tips and nodes of the tropical hardwoods Corymbia torelliana× C. citriodora and Khaya senegalensis. Plant Cell, Tissue and Organ Culture (PCTOC), 109(2), 341-352. https://doi.org/10.1007/s11240-011-0099-0
  23. Hussien, E. T., Ahmed, M. F., Abdel-Gaber, R., Balogun, O. S., & Razik, A. B. A. (2024). Synthesis and characterization of artificial seeds plantlets of in vitro male Populus alba. Plant Cell, Tissue and Organ Culture (PCTOC), 156(2), 37. https://doi.org/10.1007/s11240-023-02664-7
  24. Javed, S. B., Alatar, A. A., Anis, M., & Faisal, M. (2017). Synthetic seeds production and germination studies, for short term storage and long distance transport of Erythrina variegata L.: A multipurpose tree legume. Industrial Crops and Products, 105, 41-46. https://doi.org/10.1016/j.indcrop.2017.04.053
  25. Kępińska-Pacelik, J., & Biel, W. (2026). Hawthorn (Crataegus monogyna Jacq.): A review of therapeutic potential and applications. Molecules, 31(2), 226. https://doi.org/10.3390/molecules31020226
  26. Khan, M. I., Ahmad, N., Anis, M., Alatar, A. A., & Faisal, M. (2018). In vitro conservation strategies for the Indian willow (Salix tetrasperma Roxb.), a vulnerable tree species via propagation through synthetic seeds. Biocatalysis and Agricultural Biotechnology, 16, 17-21. https://doi.org/10.1016/j.bcab.2018.07.002
  27. Khanam, M. N., Javed, S. B., Ahmad, N., & Anis, M. (2021). Encapsulation of nodal segments of Allamanda cathartica for short-term storage and germplasm exchange. Plant Cell, Tissue and Organ Culture, 145(2), 435-443. https://doi.org/10.1007/s11240-021-02019-0
  28. Kocak, M., Sevindik, B., Izgu, T., Tutuncu, M., & Mendi, Y. Y. (2019). Synthetic seed production of flower bulbs. In Faisal, M., Alatar, A. A. (Ed.), Synthetic Seeds: Germplasm Regeneration, Preservation and Prospects (pp. 283-299). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-030-24631-0_12
  29. Koçak, M., Yılmaz, M. C., Kuzğun, C., Sirke, S. T., & Yildiz, M. (2025). Synthetic seed production in Crataegus monogyna L. and prediction of regeneration of synthetic seeds with machine learning algorithms. Plant Cell, Tissue and Organ Culture, 161(1), 22. https://doi.org/10.1007/s11240-025-03049-8
  30. Lloyd, G., & McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. In Proceedings of the International Plant Propagator's Society, 30, 421–427.
  31. Maghchiche, A. (2025). Hawthorn (Crataegus Spp.): Unlocking its potential for health and environmental sustainability–a comprehensive. Journal of Bioresource Management, 12(3), 1.
  32. Majd, A., Zandi, N., Arbabian, S., & Sharifnia, F. (2016). The comparative study of synthetic seed production in some species of Crataegus by meristem culture and somatic embryos. Cell and Tissue Journal, 7(2), 121-129.
  33. Manjkhola, S., Dhar, U., & Joshi M. (2005). Organogenesis, embryogenesis, and synthetic seed production in Arnebia euchroma—a critically endangered medicinal plant of the Himalaya. In Vitro Cellular and Developmental Biology, 41(3), 244–248. https://doi.org/10.1079/ivp2004612
  34. Martinelli, F., Perrone, A., Yousefi, S., Papini, A., Castiglione, S., Guarino, F., & Salami, S. (2021). Botanical, phytochemical, anti-microbial and pharmaceutical characteristics of hawthorn (Crataegus monogyna jacq.), Rosaceae. Molecules 26(23) 7266.
  35. Muradoğlu, F., Gürsoy, S., & Güler, E. (2021). Multivariate analysis revealed the morphological variability among Crataegus species. Yuzuncu Yıl University Journal of Agricultural Sciences, 31(4), 961-972. https://doi.org/10.29133/yyutbd.974538
  36. Qahtan, A. A., Alatar, A. A., & Faisal, M. (2023). In vitro regeneration, phytochemical profiling and antioxidant activity in Ruta chalepensis plants established from alginate encapsulated synthetic seeds. South African Journal of Botany, 161, 575-585. https://doi.org/10.1016/j.sajb.2023.08.065
  37. Rihan, H. Z., Kareem, F., El-Mahrouk, M. E., & Fuller, M. P. (2017). Artificial seeds (principle, aspects and applications). Agronomy, 7(4), 71. https://doi.org/10.3390/agronomy7040071
  38. Saadat, S., Majd, A., Naseri, L., Iranbakhsh, A., & Jafari, M. (2023). Optimization of somatic embryogenesis, synthetic seed production, and evaluation of genetic fidelity in Teucrium polium L. In Vitro Cellular & Developmental Biology, 59(4), 483-496. https://doi.org/10.1007/s11627-023-10360-6
  39. Saiprasad, G. (2001). Artificial seeds and their applications. Resonance, 6, 39–47. https://doi.org/10.1007/bf02839082
  40. Sakhanokho, H. F., Pounders, C. T., & Blythe, E. K. (2013). Alginate encapsulation of begonia microshoots for short‐term storage and distribution. The Scientific World Journal, 2013(1), 341568.
  41. Sarmah, D. K., Borthakur M., & Borua P. K. (2010). Artificial seed production from encapsulated PLBs regenerated from leaf base of Vanda coerulea Grifft. ex. Lindl. - an endangered orchid. Current Science, 98(5), 686–690.
  42. Sharma, S., Shahzad, A., & Da Silva J. A. T. (2013). Synseed technology—a complete synthesis. Biotechnology Advances, 31, 186–207. https://doi.org/10.1016/j.biotechadv.2012.09.007
  43. Siddique, I., & Bukhari, N. A. W. (2018). Synthetic seed production by encapsulating nodal segment of Capparis decidua (Forsk.), in vitro regrowth of plantlets and their physio biochemical studies. Agroforestry Systems, 92(6), 1711-1719. https://doi.org/10.1007/s10457-017-0120-7
  44. Singh, R.P., Prasad, P. V., & Reddy, K. R. (2013). Impacts of changing climate and climate variability on seed production and the seed industry. Advances in Agronomy, 118, 49–110. https://doi.org/10.1016/b978-0-12-405942-9.00002-5
  45. Sota, V., Benelli, C., Myrselaj, M., Kongjika, E., & Gruda, N. S. (2023). Short-term conservation of Juglans regia L. via synthetic seed technology. Horticulturae, 9(5), 559.
  46. Stabnikova, O., Stabnikov, V., Paredes-López, O. (2025). Wild Edible Plants, Berries, Mushrooms, and Seaweeds in Food Production. In Gubsky, S., Stabnikova, O., Stabnikov, V., Paredes-López, O. (Eds), Wild Edible Plants (pp. 1-47). CRC Press. https://doi.org/10.1201/9781003486794-1
  47. Tabassum, B., Nasir, I. A., Farooq, A. M., Rehman, Z., Latif, Z., & Husnain, T. (2010). Viability assessment of in vitro produced synthetic seeds of cucumber. African Journal of Biotechnology, 9(42), 7026–7032. https://doi.org/10.1201/9781003486794
  48. Tassell, M. C., Kingston, R., Gilroy, D., Lehane, M., & Furey, A. (2010). Hawthorn (Crataegus spp.) in the treatment of cardiovascular disease. Pharmacognosy Reviews, 4(7), 32–41. https://doi.org/10.4103/0973-7847.65324
  49. Yalçın Dokumacı, K., Uslu, N., Hacıseferoğulları, H., & Örnek, M. N. (2021). Determination of some physical and chemical properties of common hawthorn (Crataegus Monogyna Jacq. Var. Monogyna). Erwerbs-Obstbau, 63(1), 99-106. https://doi.org/10.1007/s10341-021-00545-x
  50. Yanakieva, V. (2023). Study on the antimicrobial activity of medicinal plant extracts and emulsion products with integrated herbal extracts. Agriculture & Forestry, 69(4). https://doi.org/10.17707/agricultforest.69.4.06
  51. Zhang, Z., Ho, W. K. K., Huang, Y., & Chen, Z. Y. (2002). Hypocholesterolemic activity of hawthorn fruit is mediated by regulation of cholesterol- 7α-hydroxylase and acyl CoA: cholesterol acyltransferase. Food Research International, 35, 885–891. https://doi.org/10.1016/s0963-9969(02)00099-6
  52. Daud, M., Taha, M. Z., & Hasbullah, A.Z. (2008). Artificial seed production from encapsulated micro shoots of Sainpaulia ionantha Wendl. (African Violet). Journal of Applied Sciences, 8, 4662–4667. https://doi.org/10.3923/jas.2008.4662.4667