Journals / Black Sea Journal of Agriculture / 2026 / Cilt: 9 Sayı: 5

Determination of the Persistence of Some Entomopathogenic Fungi and Wettable Powder Formulations on Concrete Surfaces against Major Stored Product Pests

Cebrail Barış(Kahramanmaraş sütçü imam üniversitesi)0000-0002-5895-0151
Pages
733–739
Publication Date
2026-09-15
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, WP1 showed the highest efficacy and persistence, indicating the potential of fungal wettable powder formulations for long-term biological control of stored-product insect pests.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the entomopathogenic fungi Beauveria bassiana Vuillemin (Hypocreales: Cordycipitaceae) and Metarhizium robertsii Sorokin (Hypocreales: Clavicipitaceae) and their wettable powder (WP) formulations were evaluated for efficacy and persistence on concrete surfaces against major stored-product insect pests.
📊
Key Findings & Data:B. bassiana (5-4) and M. robertsii (S3) isolates and their formulations (WP1 and WP2) were tested after 0, 30, 60, and 90 days of surface exposure.
💡
Conclusion & Impact:Overall, WP1 showed the highest efficacy and persistence, indicating the potential of fungal wettable powder formulations for long-term biological control of stored-product insect pests.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Wettable powder · Concrete surface · Entomopathogenic fungi

Abstract

In this study, the entomopathogenic fungi Beauveria bassiana Vuillemin (Hypocreales: Cordycipitaceae) and Metarhizium robertsii Sorokin (Hypocreales: Clavicipitaceae) and their wettable powder (WP) formulations were evaluated for efficacy and persistence on concrete surfaces against major stored-product insect pests. B. bassiana (5-4) and M. robertsii (S3) isolates and their formulations (WP1 and WP2) were tested after 0, 30, 60, and 90 days of surface exposure. Adults of Rhyzopertha dominica (F.,1792) (Coleoptera: Bostrichidae) and Sitophilus oryzae (L.,1763) (Coleoptera: Curculionidae) and third-instar larvae of Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae) were exposed to treated surfaces at 9×1010 conidia/m² under controlled laboratory conditions (25±2 °C, 65±5% RH, darkness). Persistence generally declined with increasing exposure time; however, formulated products retained efficacy better than unformulated spores. WP1 and WP2 maintained significant mortality against P. interpunctella and R. dominica after 30 days, while WP1 remained effective for up to 60 days. Mortality of S. oryzae was low throughout the study and decreased markedly after 30 days. Overall, WP1 showed the highest efficacy and persistence, indicating the potential of fungal wettable powder formulations for long-term biological control of stored-product insect pests.

Özet

In this study, the entomopathogenic fungi Beauveria bassiana Vuillemin (Hypocreales: Cordycipitaceae) and Metarhizium robertsii Sorokin (Hypocreales: Clavicipitaceae) and their wettable powder (WP) formulations were evaluated for efficacy and persistence on concrete surfaces against major stored-product insect pests. B. bassiana (5-4) and M. robertsii (S3) isolates and their formulations (WP1 and WP2) were tested after 0, 30, 60, and 90 days of surface exposure. Adults of Rhyzopertha dominica (F.,1792) (Coleoptera: Bostrichidae) and Sitophilus oryzae (L.,1763) (Coleoptera: Curculionidae) and third-instar larvae of Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae) were exposed to treated surfaces at 9×1010 conidia/m² under controlled laboratory conditions (25±2 °C, 65±5% RH, darkness). Persistence generally declined with increasing exposure time; however, formulated products retained efficacy better than unformulated spores. WP1 and WP2 maintained significant mortality against P. interpunctella and R. dominica after 30 days, while WP1 remained effective for up to 60 days. Mortality of S. oryzae was low throughout the study and decreased markedly after 30 days. Overall, WP1 showed the highest efficacy and persistence, indicating the potential of fungal wettable powder formulations for long-term biological control of stored-product insect pests.

🔖

Cite This Article

Barış, C. (2026). Determination of the Persistence of Some Entomopathogenic Fungi and Wettable Powder Formulations on Concrete Surfaces against Major Stored Product Pests. Black Sea Journal of Agriculture, Cilt: 9 Sayı: 5, 733–739. https://doi.org/10.47115/bsagriculture.1974767

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (36)

  1. Andrić, G., Kljajić, P., & Pražić-Golić, M. (2014). Residual efficacy of cypermethrin and pirimiphos-methyl against Sitophilus granarius (L.) and Plodia interpunctella (Hübner) on concrete surface. Pesticidi i Fitomedicina, 29, 275-281.
  2. Arthur, F. H. (1994). Residual efficacy of cyfluthrin emulsifiable concentrate and wettable powder formulations on porous concrete and on concrete sealed with commercial products prior to insecticide application. Journal of Stored Product Research, 30(1), 79-86.
  3. Arthur, F. H. (1996). Grain protectants: current status and prospects for the future. Journal of Stored Product Research, 32(4), 293-302.
  4. Arthur, F. H. (1998). Residual toxicity of cyfluthrin wettable powder against Tribolium confusum (Coleoptera: Tenebrionidae) exposed for short time intervals on concrete. Journal of Stored Product Research, 34(1), 19-25.
  5. Arthur, F. H. (2008). Efficacy of chlorfenapyr against Tribolium castaneum and Tribolium confusum (Coleoptera: Tenebrionidae) adults exposed on concrete, vinyl tile, and plywood surfaces. Journal of Stored Product Research, 44(2), 145-151.
  6. Arthur, F. H. (2012). Aerosols and contact insecticides as alternatives to methyl bromide in flour mills, food production facilities, and food warehouses. Journal of Pesticide Science, 85(3), 323-329.
  7. Arthur, F. H., Liu, S., Zhao B., & Phillips T. W. (2009). Residual efficacy of pyriproxyfen and hydroprene applied to wood, metal and concrete for control of stored‐product insects. Pest Management Science: formerly Pesticide Science, 65(7), 791-797.
  8. Athanassiou, C. G., & Palyvos, N. E. (2006). Laboratory evaluation of two diatomaceous earth formulations against Blattisocius keegani Fox (Mesostigmata, Ascidae) and Cheyletus malaccensis Oudemans (Prostigmata: Cheyletidae). Biological Control, 38(3), 350-355.
  9. Athanassiou, C. G., & Steenberg, T. (2007). Insecticidal effect of Beauveria bassiana (Balsamo) Vuillemin (Ascomycota: Hypocreales) in Combination with three diatomaceous earth formulations against Sitophilus granarius (L.) (Coleoptera: Curculionidae). Biological Control, 40(3), 411-416.
  10. Athanassiou, C. G., Kavallieratos, N. G., Vayias, B. J., Tsakiri, J. B., Mikeli, N. H., Meletsis, C. M., & Tomanović, Ž. (2008). Persistence and efficacy of Metarhizium anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes) and diatomaceous earth against Sitophilus oryzae (L.) (Coleoptera: Curculionidae) and Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) on wheat and maize. Crop Protection, 27(10), 1303-1311.
  11. Athanassiou, C. G., Rumbos, C. I., Sakka, M., Potin, O., Storm, C., & Dillon, A. B. (2017). Delivering Beauveria bassiana with electrostatic powder for the control of stored‐product beetles. Pest Management Science 73(8), 1725-1736.
  12. Barış C., & Er, M. K. (2025). Mortality effect of wettable powder formulations containing entomopathogenic fungal spores against some stored-product pests. Turkish Journal of Entomology, 49(2), 103-115. https://doi.org/10.16970/entoted.1599102
  13. Barış, C., & Er, M. K. (2021). Examining some cereals for mass production of Beauveria bassiana conidia by solid state fermentation. KSU Journal of Agriculture and Nature, 24(6), 561-567.
  14. Batta, Y. A. (2005). Control of the Lesser Grain Borer (Rhyzopertha dominica (F.), Coleoptera: Bostrichidae) by Treatments with Residual Formulations of Metarhizium anisopliae (Metschnikoff) Sorokin (Deuteromycotina: Hyphomycetes). Journal of Stored Product Research, 41(2), 221-229. https://doi.org/10.1016/j.jspr.2004.03.007
  15. Batta, Y. A. (2008). Control of main stored-grain insects with new formulations of entomopathogenic fungi in diatomaceous earth dusts. International Journal of Food Engineering, 4(1), 9.
  16. Cojanu, D., Lumînare, M. C., Dinu, M. M., & Fatu, A. C. (2022). Pathogenicity of Beauveria bassiana, B. pseudobassiana, and Metarhizium anisopliae indigenous isolates against Plodia interpunctella and Galleria mellonella in laboratory assays. Scientific Bulletin Series Food Biotechnologies, 26(2), 14.
  17. Collins, P. J., Nayak, M. K., & Kopittke, R. (2000). Residual efficacy of four organophosphate insecticides on concrete and galvanized steel against three liposcelid psocid species (Psocoptera: Liposcelidae) infesting stored products. Journal of Economic Entomology, 93(4), 1357-1363.
  18. Er, M. K., Tunaz, H., Ücük, C., Barış, C., & Işıkber, A. A. (2016). Occurrence of entomopathogenic fungi on insect pests of stored wheat and maize in Central and South Anatolia in Turkey. Turkish Journal of Entomology, 40(3), 249-263. https://izlik.org/JA99SP89EH
  19. Ferizli, A. G., Beris, G., & Baspinar, E. (2005). Mortality and F1 production of Rhyzopertha dominica (F.) on wheat treated with diatomaceous earth; impact of biological and environmental parameters on efficacy. Journal of Pest Science, 78(4), 231-238.
  20. George, C. G., Maria, S. K., & Christos, A. G. (2018). Efficacy of Beauveria bassiana in combination with an electrostatically charged dust for the control of major stored-product beetle species on concrete. Journal of Stored Products Research, 79, 139-143.
  21. Ghimire, M. N., Arthur, F. H., Myers, S. W., & Phillips, T. W. (2016). Residual efficacy of deltamethrin and β-cyfluthrin against Trogoderma variabile and Trogoderma inclusum (Coleoptera: Dermestidae). Journal of Stored Products Research, 66, 6-11.
  22. Henteş, S., & Işikber, A. A. (2025). Comparison of the resistance of various food packaging materials to stored product insect pests. Journal of Stored Products Research, 114, 102781. https://doi.org/10.1016/j.jspr.2025.102781
  23. Jankov, D., Inđić, D., Kljajić, P., Almaši, R., Andrić, G., Vuković, S., & Grahovac, M. (2013). Initial and residual efficacy of insecticides on different surfaces against rice weevil Sitophilus oryzae (L.). Journal of Pest Science, 86(2), 211-216. https://doi.org/10.1007/s10340-012-0469-3
  24. Kavallieratos, N. G., Athanassiou, C. G., Aountala, M. & Kontodimas, D. C. (2014). Evaluation of the entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for control of Sitophilus oryzae. Journal of Food Protection, 77(1), 87-93. https://doi.org/10.4315/0362-028X.JFP-13-196
  25. Kavallieratos, N. G., Athanassiou, C. G., Michalaki, M. P., Batta, Y. A., Rigatos, H. A., Pashalidou, F. G., Balotis, G. N., Tomanovic, Z., & Vayias, B. (2006). Effect of the combined use of Metarhizium anisopliae (Metschinkoff) Sorokin and diatomaceous earth for the control of three stored-product beetle species. Crop Protection, 25(10), 1087-1094. https://doi.org/10.1016/j.cropro.2006.02.009
  26. Lord, J. C. (2001). Desiccant dusts synergize the effect of Beauveria bassiana (Hyphomycetes:Moniliales) on stored-grain beetles. Journal of Economic Entomology, 94(2), 367-372. https://doi.org/10.1603/0022-0493-94.2.367
  27. Michalaki, M. P., Athanassiou, C. G., Teenberg, T. & Buchelos, C. T. (2007). Effect of Paecilomyces fumosoroseus (Wise) Brown and Smith (Ascomycota: Hypocreales) alone or in combination with diatomaceous earth against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae) and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Biological Control, 40(2), 280-286.
  28. Padin, S., Dal Bello, G., & Fabrizio, M. (2002). Grain loss caused by Tribolium castaneum, Sitophilus oryzae and Acanthoscelides obtectus in stored durum wheat and beans treated with Beauveria bassiana. Journal of Stored Product Research, 38(1), 69-74. https://doi.org/10.1016/S0022-474X(00)00046-1
  29. Rice, W. C., & Cogburn, R. R. (1999). Activity of the Entomopathogenic Fungus Beauveria bassiana (Deuteromycota: Hyphomycetes) against Three Coleopteran Pests of Stored Grain. Journal of Economic Entomology, 92(3), 691-694. https://doi.org/10.1093/jee/92.3.691
  30. Rumbos C. I. & Athanassiou, C. G. (2017). The use of entomopathogenic nematodes in the control of stored-product insects. Journal of Pest Science, 90(1), 39-49. https://doi.org/10.1007/s10340-016-0795-y
  31. Vassilakos, T. N., Athanassiou, C. G., Kavallieratos, N. G., & Vayias, B. J. (2006). Influence of temperature on the insecticidal effect of Beauveria bassiana in combination with diatomaceous earth against Rhyzopertha dominica and Sitophilus oryzae on stored wheat. Biological Control, 38(2), 270-281.
  32. Wakil, W., Kavallieratos, N. G., Eleftheriadou, N., Riasat, T., Ghazanfar, M. U., Rasool, K. G., & Aldawood, A. S. (2023). The potential of two entomopathogenic fungi and enhanced diatomaceous earth mixed with abamectin: A comprehensive study on mortality, progeny production, application method, and surface application against Tribolium castaneum. Pathogens, 12(6), 773. https://doi.org/10.3390/pathogens12060773
  33. Wakil, W., Riasat, T., & Ashfaq, M. (2012). Residual efficacy of thiamethoxam, Beauveria bassiana (Balsamo) Vuillemin, and diatomaceous earth formulation against Rhyzopertha dominica F. (Coleoptera: Bostrychidae). Journal of Pest Science, 85(3), 341-350. https://doi.org/10.1007/s10340-011-0408-8
  34. White, N. D. G. (1982). Effectiveness of malathion and pirimiphosmethyl applied to plywood and concrete to control Prostepahnus truncatus (Coleoptera: Bostrichidae). Proceedings of the Entomological Society of Ontario, 113, 65-69.
  35. Williams, P., Semple, R. L., & Amos, T. G. (1982). Relative toxicity and persistence of one carbamate and three organophosphate insecticides on concrete, wood and iron surfaces for control of grain insects. General and Applied Entomology, 14, 35-40.
  36. Yasir, M., Mankin, R. W., ul Hasan, M., & Sagheer, M. (2020). Residual efficacy of novaluron applied on concrete, metal, and wood for the control of stored product coleopteran pests. Insects, 12(1), 7. https://doi.org/10.3390/insects12010007