Journals / Bitlis Eren Üniversitesi Fen Bilimleri Dergisi / 2026 / Volume: 11 Issue: 2

Investigation of Biological and Chemical Effects of Extracts from Arum rupicola Boiss. var. rupicola

Arum rupicola’dan Elde Edilen Özütlerin Biyolojik ve Kimyasal Etkilerinin Araştırılması

Mehmet Rıza Kıvanç(VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ)0000-0002-9667-1225
Pages
—
Publication Date
2022-06-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, it was revealed that the five extracts from A. rupicola had a variety of phenolic compositions and fatty acids, and they also possess antioxidant effects and antibacterial activities.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The present study aimed to evaluate the fatty acid and phenolic profiles, and antioxidant and antibacterial activities of A. rupicola.
📊
Key Findings & Data:For this purpose, five extracts with different polarities were obtained using the partition method, and then the chemical analysis of these five extracts was performed with GC-MS and HPLC-TOF analyses.
💡
Conclusion & Impact:In this study, it was revealed that the five extracts from A. rupicola had a variety of phenolic compositions and fatty acids, and they also possess antioxidant effects and antibacterial activities.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Arum rupicola · Fatty Acids · Antioxidant Activity

Abstract

The present study aimed to evaluate the fatty acid and phenolic profiles, and antioxidant and antibacterial activities of A. rupicola. For this purpose, five extracts with different polarities were obtained using the partition method, and then the chemical analysis of these five extracts was performed with GC-MS and HPLC-TOF analyses. According to these data, various fatty acids and phenolic contents were observed. Furthermore, the antioxidant studies were performed with total phenolic compounds and ABTS, while antibacterial studies were performed with the microdilution test and the disc diffusion test using three bacteria, one gram-positive and two gram-negative bacteria. The antioxidant and antibacterial tests revealed that A. rupicola is both an antioxidant and an antibacterial plant. According to the obtained results, the microdilution test had higher effectiveness than the disc diffusion test. The inhibition percent vs. extract concentration graph showed that MH was the best inhibitor. In this study, it was revealed that the five extracts from A. rupicola had a variety of phenolic compositions and fatty acids, and they also possess antioxidant effects and antibacterial activities.

Özet

Bu çalışma A. rupicola’nın yağ asit ve fenolik içeriklerini, antioksidant ve antibakteriyel aktivitelerini ortaya çıkarmayı amaçlamaktadır. Bu amaçla, partisyon yöntemi kullanılarak farklı polariteli beş özüt elde edildi ve sonrasında bu özütlerin GC-MS ve HPLC-TOF analizleriyle kimyasal analizleri yapıldı. Bu analizlere göre, çeşitli yağ asitleri ve fenolik içerikleri belirlendi. Dahası, antibakteriyel çalışmalar üç bakteri türü biri gram-pozitif ve ikisi gram-negatifle mikro seyreltme ve disk yayılım metodları kullanılarak, antioksidan çalışmaları toplam fenolik bileşik ve ABTS kullanılarak gerçekleştirildi. Antioksidan ve anti bakteriyel testler A. rupicola’nın hem antioksidan hemde antibakteriyel bir bitki olduğunu ortaya çıkardı. Ayrıca, elde edilen bulgulara göre mikro seyreltme testinin disk yayılım yönteminden daha etkili olduğunu gösterdi. Öte yandan, % inhibisyona karşı özüt konsantrasyon grafiği MH özütünün en etkili inhibitör olduğunu gösterdi. Bu çalışmayla A. rupicola’dan elde edilen beş özütün çeşitli fenolik bileşikler ve yağ asitlerinin olduğunu ve de bunların antioksidan ve antibakteriyeler aktivitelerinin olduğunu ortaya çıkarmıştır.

🔖

Cite This Article

Kıvanç, M. R. (2022). Investigation of Biological and Chemical Effects of Extracts from Arum rupicola Boiss. var. rupicola. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, Volume: 11 Issue: 2. https://doi.org/10.17798/bitlisfen.1061984

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (41)

  1. B. Schaal, “Plants and people: Our shared history and future,” Plants People Planet, vol. 1, pp. 14–19, 2019. https://doi.org/10.1002/ppp3.12
  2. P. C. Boyce, “Arum - A Decade of Change,” Aroideana, vol. 29, pp. 132-137, 2006.
  3. R. Govaerts and D. G. Frodin, World checklist and bibliography of Araceae (and Acoraceae). Kew: The Board of Trustees of the Royal Botanic Gardens 2002.
  4. W. Lobin, M. Neumann, J. Bogner and P. C. Boyce, “A new Arum species (Areae, Araceae) from NE Turkey and Georgia,” Willdenowia, vol. 37 pp. 445, 2007. https://doi.org/10.3372/wi.37.37206
  5. R. Farahmandfar, R. Esmaeilzadeh Kenari, M. Asnaashari, D. Shahrampour and T. Bakhshandeh, “Bioactive compounds, antioxidant and antimicrobial activities of Arum maculatum leaves extracts as affected by various solvents and extraction methods,” Food Sci Nutr vol. 7 pp. 465–475, 2019. https://doi.org/10.1002/fsn3.815
  6. K. Alpınar, A. Güner, S. Aslan, T. Ekim, M. Vural and M. T. Babaç, Türkiye Bitkileri Listesi (Damarlı Bitkiler), İstanbul, 2012.
  7. H. Yıldırım and Y. Altıoğlu, “Türkiye için yeni bir takson kaydı: Arum sintenisii (Engl.) P.C.Boyce (Araceae),” Bağbahçe Bilim Dergisi, vol. 3, pp. 47–54, 2016.
  8. T. Baytop, Türkçe bitki adlari sözlüğü (14th ed.), Ankara, 2015.
  9. G. Ágoston and B. A. Masters, Encyclopedia of the Ottoman Empire, New York, NY., 2009.
  10. B. Kimmerling and J. S. Migdal, The palestinian people: A history, Harvard University Press, 2003.
  11. R. Al-Ramahi, N. Jaradat, A. N. Zaid, F. F. Vincieri and M. Asmaa, “Medicinal herbs and methodologies for their pharmaceutical compounding in the west bank/Palestine,” Complement Ther Clin Pract, vol. 20, no. 4, pp. 280-284, 2014. https://doi.org/10.1016/j.ctcp.2014.06.001
  12. E. Tuzlacı, P. Eryaşar-Aymaz, “Turkish folk medicinal plants, part IV: Gonen (Balıkesir),” Fitoterapia vol. 72, no. 4, pp. 323–343, 2001. https://doi.org/10.1016/S0367-326X(00) 00277-X
  13. D. Satyajit, Z. Sarker, A. Latif and I. Gray, Natural product isolation. Second edition, Humana Press, 2006. https://doi.org/10.1021/np078142v
  14. S. R. Kanatt, R. Chander and A. Sharma, “Chitosan and mint mixture: A new preservative for meat and meat products,” Food Chem, vol. 107, pp. 845–852, 2008. https://doi.org/10.1016/j.foodchem.2007.08.088
  15. G. Işcan, N. Kirimer, M. Kürkcüoğlu, K. H. C. Başer and F. Demirci,. “Antimicrobial screening of Mentha piperita essential oils,” J Agric Food Chem vol. 50, pp. 3943–3946, 2002. https://doi.org/10.1021/jf011476k
  16. M. D. Soković, J. Vukojević, P. D. Marin, D. D. Brkić, V. Vajs, L. J. L. D. van Griensven, “Chemical composition of essential oils of Thymus and Mentha species and their antifungal activities,” Molecules vol. 14, pp. 238–249. https://doi.org/10.3390/molecules14010238
  17. S. A. Yang, S. K. Jeon, E. J. Lee, C. H. Shim and I. S. Lee, Comparative study of the chemical composition and antioxidant activity of six essential oils and their components,” Nat Prod Res, vol. 24, pp. 140–151. https://doi.org/10.1080/14786410802496598
  18. M. Bajpai, A. Pande, S. K. Tewari, D. Prakash, “Phenolic contents and antioxidant activity of some food and medicinal plants,” Int J Food Sci Nutr, vol. 56, pp. 287–291, 2005. https://doi.org/10.1080/09637480500146606
  19. C. N. Cutter, “Antimicrobial effect of herb extracts against Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella typhimurium associated with beef,” J Food Prot vol. 63pp. 601–607, 2000. https://doi.org/10.4315/0362-028x-63.5.601
  20. A. Amal, A. Ashraf and E. S. Hossam, “Chemical compositions and antioxidant/antimicrobial activities of kaffmaryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica) cultivated in Egypt,” Biyoloji Bilimleri Arastirma Dergisi, vol. 2, no. 2, pp. 71–79, 2009. https://doi.org/10.3989/gya.064509
  21. A. P. Desbois and V. J. Smith, “Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential,” Appl Microbiol Biotechnol, vol. 85, pp. 1629–1642, 2010. https://doi.org/10.1007/s00253-009-2355-3
  22. C. L. Fischer, D. R. Drake, D. V. Dawson, D. R. Blanchette, K. A. Brogden and P. W. Wertz, “Antibacterial activity of sphingoid bases and fatty acids against Gram-positive and Gram-negative bacteria,” Antimicrob Agents Chemother, vol. 56 pp. 1157–1161, 2012. https://doi.org/10.1128/AAC.05151-11
  23. E. Karimi, H. Z. Jaafar, A. Ghasemzadeh and M. Ebrahimi, Fatty acid composition, antioxidant and antibacterial properties of the microwave aqueous extract of three varieties of Labisia pumila Benth.,” Biol Res, vol. 48, no. 1, pp. 9, 2015. https://doi.org/10.1186/0717-6287-48-9
  24. N. Özok, and I. Güneş, “Streptozotosin kaynaklı diyabetik sıçanlarda Arum rupicola'nın in vivo antioksidan potansiyeli,” Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 8, no. 3, pp. 866-874, 2019.
  25. Y. Alan, “Arum elongatum Steven Ekstraktlarının Fenolik Madde Miktarı ve Biyolojik Aktivitelerinin İncelenmesi,” Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, vol. 7, no. 2, pp. 370-379, 2018.
  26. M. R. Kıvanç and V. Türkoglu, “Investigation of the effects of natural compounds isolated from Arum rupicola var. rupicola on glutathione reductase enzyme purified from bovine liver,” Biomed Chromatogr, vol. 33, pp. e4560, 2019. https://doi.org/10.1002/bmc.4560
  27. A. Tüfekçi, H. Akşit, F. Gül and İ. Demirtaş, “Determination of Phenolic Profile of Cirsium arvense (L.) Scop. Subsp. vestitum (Wimmer et Grab.) Petrak Plant,” Eurasian J Bio Chem Sci, vol. 1, no. 2, pp. 33-36, 2018.
  28. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Rad Bio Med, vol. 26, pp. 1231–7, 1999. https://doi.org/10.1016/s0891-5849(98)00315-3
  29. K. Kalia, K. Sharma, H. P. Singh and B. Singh, “Effects of extraction methods on phenolic contents and antioxidant activity in aerial parts of Potentilla atrosanguinea Lodd. and quantification of its phenolic constituents by RP-HPLC,” J Agric Food Chem, vol. 56, pp. 10129–10134, 2008. https://doi.org/10.1021/jf802188b
  30. F. Cömert Önder, N. Doğrular, E. Güzdüzalp, S. Barlak and M. Ay, “A Comparative Study of Three Brassicaceae Vegetables Grown in Canakkale: Determination of Total Phenolic Content and Antioxidant Activity of Pulp and Juice Samples of Radish (Raphanus sativus L.), Cabbage (Brassica oleracea L. var capitata L) and Cauliflower (Brassica oleracea L.),” Çanakkale Onsekiz Mart Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol. 6, pp. 30-38, 2020. https://doi.org/10.28979/comufbed.633456
  31. Y. S. Velioglu, G. Mazza, L. Gao and B. D. Oomah, “Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products,” J Agric Food Chem, vol. 46, pp. 4113–4117, 1998. https://doi.org/10.1021/jf9801973
  32. F. Cömert Önder, M. Ay and S. D. Sarker, “Comparative study of antioxidant properties and total phenolic content of the extracts of Humulus lupulus L. and quantification of bioactive components by LC−MS/MS and GC−MS,” J Agric Food Chem, vol. 61, pp. 10498−10506, 2013. https://doi.org/10.1021/jf4031508
  33. O. Ozay, P. Ilgin, H. Ozay, Z. Gungor, B. Yılmaz and M. R. Kıvanç, “The preparation of various shapes and porosities of hydroxyethyl starch/p(HEMA-co-NVP) IPN hydrogels as programmable carrier for drug delivery,” J Macromol Sci A, vol. 57, pp. 379–387, 2020. https://doi.org/10.1080/10601325.2019.1700803
  34. W. W. Christie, “13-Phenyltridec-9-enoic and 15-phenylpentadec-9-enoic acids in Arum maculatum seed oil,” Eur J Lipid Sci Technol, vol. 105, pp. 779–780, 2013. https://doi.org/10.1002/ejlt.200300865
  35. M. M. Farid, S. R. Hussein, L. F. İbrahim, M. A. El Desouky, A. M. Elsayed, A. A. El Oqlah and M. M. Saker, “Cytotoxic activity and phytochemical analysis of Arum palaestinum Boiss,” Asian Paci J Trop Biomed, vol. 5, pp. 944–947, 2015. https://doi.org/10.1016/j.apjtb.2015.07.019
  36. E. Yabalak, “Antioxidant Activity and Chemical Composition of Methanolic Extract from Arum Dioscoridis SM. var. Dioscoridis and Determination of Mineral and Trace Elements,” Journal of the Turkish Chemical Society, Section A: Chemistry, pp. 205–218, 2017. https://doi.org/10.18596/jotcsa.350370
  37. A. Zargoun, A. Mohammed, K. Abdelshafeek, A. Ibansharrada and A. Alomari, “GC/MS Analysis of Lipid Constituents and Antimicrobial Activity of Arum cyrinaicum Extracts,” Egypt J Chem, vol. 63, no. 12, pp. 4889-4910, 2020. https://doi.org/10.21608/ejchem.2020.30199.2647
  38. H. Uguzlar, E. Maltas and S. Yildiz, “Antioxidant Activity and Fatty Acid Compositions of Arum dioscoridis Extracts,” Biosci Biotechnol Res Asia, vol. 8, pp. 75–82, 2011. https://doi.org/10.13005/bbra/825
  39. F. Karahan, M. Kulak, E. Urlu, H. G. Gözüacik, T. Böyümez, N. Şekeroğlu and I. H. Doğantürk, “Total phenolic content, ferric reducing and DPPH scavenging activity of Arum dioscoridis,” Nat Prod Res, vol. 29, pp. 1678–1683, 2015. https://doi.org/10.1080/14786419.2014.991320
  40. N. Jaradat and M. Abualhasan, “Comparison of Phytoconstituents, Total Phenol Contents and Free Radical Scavenging Capacities between Four Arum Species from Jerusalem and Bethlehem,” Pharm Sci, vol. 22, pp. 120–125, 2016. https://doi.org/10.15171/PS.2016.19
  41. H. M. Jaber, K. D. Al-Hamaideh, H. I. Al-Daghistani, N. H. Amer, M. N. Nassar, S. M. Abd Al-Latif and A. H. Al-Nuaimi, “Antibacterial Activity and Chemical Composition of Arum hygrophilum Boiss Crude Extracts,” JJBS, vol. 13, no. 2, pp. 159–164, 2020. https://doi.org/10.13140/RG.2.2.30662.42562