Journals / Bitlis Eren Üniversitesi Fen Bilimleri Dergisi / 2026 / Volume: 15 Issue: 2

Physicochemical, Chemical, and Antibacterial Characterization of Lavandula angustifolia Essential Oils Cultivated in Çankırı, Türkiye

Ibrahim Filazi(ÇANKIRI KARATEKİN ÜNİVERSİTESİ, MERKEZİ ARAŞTIRMA LABORATUVARI UYGULAMA VE ARAŞTIRMA MERKEZİ)0000-0002-8194-1421
Ali Rıza Tüfekçi(CANKIRI KARATEKIN UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF CHEMISTRY)0000-0002-2951-3657
Serdal Tarhane(Çankırı Karatekin University, Sabanozu Vocational School of Health Services, Veterinary Department, Laborant and Veterinary Health Program)0000-0003-0806-7659
İnan Dursun(Bingol University, Department of Beekeeping, Vocational School of Food, Agriculture and Livestock, Bingol University, Center of Research and Application Center)0000-0003-1717-8166
Taşkın Doğan(Çankırı Şehit Mehmet Ata Agricultural Vocational and Technical Anatolian High School)0009-0000-6648-0092
Fatih Büyük(KAFKAS UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, DEPARTMENT OF MICROBIOLOGY)0000-0003-3278-4834
Funda Oskay(ÇANKIRI KARATEKİN ÜNİVERSİTESİ, ORMAN FAKÜLTESİ, ORMAN MÜHENDİSLİĞİ BÖLÜMÜ, ORMAN ENTOMOLOJİSİ VE KORUMA ANABİLİM DALI)0000-0002-8918-5595
Pages
—
Publication Date
2026-06-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, the results confirm that Lavabdula angustifolia cultivated in Çankırı produces a French-type lavender oil of superior physicochemical and biological quality, suitable for pharmaceutical, cosmetic, and aromatherapeutic applications

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The present study investigates the physicochemical, chemical, and antibacterial properties of essential oils obtained from the flower (LF-EO) and stem-leaf (LSL-EO) fractions of Lavandula angustifolia cultivated under the semi-arid continental conditions of Çankırı, Türkiye.
📊
Key Findings & Data:The essential oils were extracted by hydrodistillation using a Neo-Clevenger apparatus and characterized in accordance with ISO 3515:2002 and related standards.
💡
Conclusion & Impact:Overall, the results confirm that Lavabdula angustifolia cultivated in Çankırı produces a French-type lavender oil of superior physicochemical and biological quality, suitable for pharmaceutical, cosmetic, and aromatherapeutic applications
🔬Design:Research Article
📍Scope:2002 Verileri
⚙️Variables:Lavandula angustifolia · Characterization · Antimicrobial activity

Abstract

The present study investigates the physicochemical, chemical, and antibacterial properties of essential oils obtained from the flower (LF-EO) and stem-leaf (LSL-EO) fractions of Lavandula angustifolia cultivated under the semi-arid continental conditions of Çankırı, Türkiye. The essential oils were extracted by hydrodistillation using a Neo-Clevenger apparatus and characterized in accordance with ISO 3515:2002 and related standards. The physicochemical parameters of LF-EO, including density (0.8937 g mL⁻¹), refractive index (1.4566), optical rotation (-9.50°), and ester value (55.04 mg KOH g⁻¹), were consistent with the ISO specifications for high-quality lavender oil. GC-MS analysis identified 98.11 % (LF-EO) and 98.42 % (LSL-EO) of total volatile constituents, revealing β-linalool (44.77 % and 34.55 %) and linalyl acetate (12.94 % and 6.60 %) as the major compounds, alongside camphor, borneol, and 1,8-cineole. The compositional profile of LF-EO corresponded to the French Maillette chemotype defined by ISO 3515:2002. Both oils exhibited notable antibacterial activity against Gram-positive and Gram-negative bacteria. LF-EO demonstrated strong inhibition against Bacillus cereus and Listeria monocytogenes (30 mm), while LSL-EO showed moderate activity against Escherichia coli (20 mm) and Salmonella typhimurium (17 mm). The enhanced antimicrobial performance of LF-EO was attributed to its high content of oxygenated monoterpenes and esters, which contribute to membrane permeability and destabilization effects. Overall, the results confirm that Lavabdula angustifolia cultivated in Çankırı produces a French-type lavender oil of superior physicochemical and biological quality, suitable for pharmaceutical, cosmetic, and aromatherapeutic applications

🔖

Cite This Article

Tarhane, A. K., Filazi, I., Tüfekçi, A. R., Tarhane, S., Dursun, İ., Doğan, T., Büyük, F., Oskay, F. (2026). Physicochemical, Chemical, and Antibacterial Characterization of Lavandula angustifolia Essential Oils Cultivated in Çankırı, Türkiye. Bitlis Eren Üniversitesi Fen Bilimleri Dergisi, Volume: 15 Issue: 2. https://doi.org/10.17798/bitlisfen.1819180

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (24)

  1. M. A. Cook and G. D. Wright, “The past, present, and future of antibiotics,” Sci. Transl. Med., vol. 14, 2022, Art. no. eabo7793, doi: 10.1126/scitranslmed.abo7793.
  2. H. Niu, J. Gu, and Y. Zhang, “Bacterial persisters: molecular mechanisms and therapeutic development,” Signal Transduct. Target Ther., vol. 9, 2024, Art. no. 174, doi: 10.1038/s41392-024-01866-5.
  3. R. Duan et al., “Efficient inactivation of antibiotic resistant bacteria by iron-modified biochar and persulfate system: potential for controlling antimicrobial resistance spread and mechanism insights,” J. Hazard. Mater., vol. 492, 2025, Art. no. 138182, doi: 10.1016/j.jhazmat.2025.138182.
  4. H. Xin, Y. Liu, Y. Xiao, M. Wen, L. Sheng, and Z. Jia, “Design and nanoengineering of photoactive antimicrobials for bioapplications: from fundamentals to advanced strategies,” Adv. Funct. Mater., vol. 34, no. 38, 2024, Art. no. 2402607, doi: 10.1002/adfm.202402607.
  5. A. Ciocarlan et al., “Chemical composition and assessment of antimicrobial activity of lavender essential oil and some by-products,” Plants, vol. 10, no. 9, 2021, Art. no. 1829, doi: 10.3390/plants10091829.
  6. S. S. Denner, “Lavandula angustifolia Miller: English lavender,” Holist. Nurs. Pract., vol. 23, no. 1, pp. 57–64, 2009, doi: 10.1097/01.HNP.0000343210.56710.fc.
  7. A. R. Tüfekçi et al., “Two new compounds from endemic Centaurea paphlagonica (Bornm.) Wagenitz and their cytotoxic activities,” Chem. Biol. Drug Des., vol. 103, no. 1, 2024, Art. no. e14409, doi: 10.1111/cbdd.14409.
  8. A. R. Tüfekçi, “Comparative determination of enzyme, insecticide, antibacterial activities of different extracts of Diplotaxis tenuifolia and chemical components analysis,” Plant Prot. Bull., vol. 65, no. 2, pp. 21–31, 2025, doi: 10.16955/bitkorb.1599639.
  9. ISO 3515:2002, Essential oils—Lavender oil (Lavandula angustifolia Mill.). International Organization for Standardization, Geneva, Switzerland, 2002.
  10. A. K. Tarhane, S. Tarhane, İ. Dursun, F. Büyük, M. R. Coşkun, and Y. Ersoy, “GC-MS analysis of chemical composition and determination of antimicrobial activity of laurel leaf extracts prepared by different methods and solvents,” Pakistan Vet. J., vol. 44, no. 3, pp. 619–628, 2024, doi: 10.29261/pakvetj/2024.207.
  11. C. Caprari et al., “Chemical profile, in vitro biological activity and comparison of essential oils from fresh and dried flowers of Lavandula angustifolia L.,” Molecules, vol. 26, no. 17, 2021, Art. no. 5317, doi: 10.3390/molecules26175317.
  12. A. B. Perović et al., “Novel hydrodistillation and steam distillation methods of essential oil recovery from lavender: a comprehensive review,” Ind. Crops Prod., vol. 211, 2024, Art. no. 118244, doi: 10.1016/j.indcrop.2024.118244.
  13. I. Betlej et al., “Phytochemical composition and antimicrobial properties of new Lavandula angustifolia ecotypes,” Molecules, vol. 29, no. 8, 2024, Art. no. 1740, doi: 10.3390/molecules29081740.
  14. G. Schneider, A. Steinbach, Á. Putics, Á. Solti-Hodován, and T. Palkovics, “Potential of essential oils in the control of Listeria monocytogenes,” Microorganisms, vol. 11, no. 6, 2023, Art. no. 1364, doi: 10.3390/microorganisms11061364.
  15. S. Roller, N. Ernest, and J. Buckle, “The antimicrobial activity of high-necrodane and other lavender oils on methicillin-sensitive and -resistant Staphylococcus aureus (MSSA and MRSA),” J. Altern. Complement. Med., vol. 15, no. 3, pp. 275–279, 2009, doi: 10.1089/acm.2008.0268.
  16. S. Chao, G. Young, C. Oberg, and K. Nakaoka, “Inhibition of methicillin-resistant Staphylococcus aureus (MRSA) by essential oils,” Flavour Fragrance J., vol. 23, no. 6, pp. 444–449, 2008, doi: 10.1002/ffj.1904.
  17. A. F. Haag, J. R. Fitzgerald, and J. R. Penadés, “Staphylococcus aureus in animals,” Microbiol. Spectr., vol. 7, no. 3, 2019, Art. no. 1128, doi: 10.1128/microbiolspec.gpp3-0060-2019.
  18. M. Kokina et al., “Characterization, antioxidant and antibacterial activity of essential oils and their encapsulation into biodegradable material followed by freeze drying,” Food Technol. Biotechnol., vol. 57, no. 2, pp. 282–289, 2019, doi: 10.17113/ftb.57.02.19.5957.
  19. Y. Nguyen and V. Sperandio, “Enterohemorrhagic E. coli (EHEC) pathogenesis,” Front. Cell. Infect. Microbiol., vol. 2, 2012, Art. no. 90, doi: 10.3389/fcimb.2012.00090.
  20. W. A. Ferens and C. J. Hovde, “Escherichia coli O157:H7: Animal reservoir and sources of human infection,” Foodborne Pathog. Dis., vol. 8, no. 4, pp. 465–487, Apr. 2011, doi: 10.1089/fpd.2010.0673.
  21. M. Soković, J. Glamočlija, P. D. Marin, D. Brkić, and L. J. L. D. van Griensven, “Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model,” Molecules, vol. 15, no. 11, pp. 7532–7546, 2010, doi: 10.3390/molecules15117532.
  22. A. I. Hussain, F. Anwar, P. S. Nigam, M. Ashraf, and A. H. Gilani, “Seasonal variation in content, chemical composition and antimicrobial and cytotoxic activities of essential oils from four Mentha species,” J. Sci. Food Agric., vol. 90, no. 11, pp. 1827–1836, 2010, doi: 10.1002/jsfa.4021.
  23. S. Burt, “Essential oils: Their antibacterial properties and potential applications in foods—A review,” Int. J. Food Microbiol., vol. 94, pp. 223–253, 2004, doi: 10.1016/j.ijfoodmicro.2004.03.022.
  24. S. B. Gök and Y. Erdoğdu, “Chemical composition and antimicrobial activity of essential oils from six lavender (Lavandula angustifolia Mill.) cultivars,” Plant Soil Environ., vol. 70, no. 2, pp. 111–123, 2024, doi: 10.17221/438/2023-PSE.