SavedAdd your journal

Journals / Acta Medica Alanya / 2026 / Cilt: 4 Sayı: 1

Antioxidant Effects of Myrtus communis L.’s Essential Oils in BEAS-2B Cells Induced by Oxidative Stress with Hydrogen Peroxide

Hidrojen Peroksit ile Okdisatif Stresin İndüklendiği BEAS-2B Hücrelerinde Myrtus communis L. Esansiyel Yağının Antioksidan Etkilerinin Araştırılması

Hayriye Zehra Ulutaş(HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, MOLEKÜLER BİYOKİMYA VE GENETİK ANABİLİM DALI)0000-0001-6553-3420
Gülay Gülbol Duran(HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, TAYFUR ATA SÖKMEN TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ BİYOLOJİ ANABİLİM DALI)0000-0002-4672-2960
Pages
21–28
Publication Date
2020-03-02
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways.
📊
Key Findings & Data:Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways.
💡
Conclusion & Impact:Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Myrtus communis L · Apopitoz · BEAS-2B

Abstract

Aim: In this study, the effects of Myrtus communis L. essential oil on the human bronchial epithelial cell line (BEAS-2B) exposed to oxidative stress with hydrogen peroxide were investigated and their effects on apoptotic pathways.

Özet

Amaç: Bu çalışmada, Myrtus communis L. esansiyel yağının, in vitro olarak hidrojen peroksit ile oksidatif strese uğratılmış insan bronşiyal epitel hücre hattında (BEAS-2B) antioksidan etkilerinin olup olmadığı ve apoptotik yolaklar üzerindeki etkileri araştırılmıştır. Materyal ve Metod: BEAS-2B hücrelerinde H2O2 ile M. communis L. esansiyel yağının uygun dozlarının belirlenmesinde MTT yöntemi kullanılmıştır. Oksidatif stres oluşumu MDA düzeyi ölçülerek belirlenmiştir. Hücreler; oksidatif strese maruz bırakılan grup (H2O2), tedavili grup (H2O2+M. communis L) ve kontrol grubu olmak üzere üç gruba ayrılmıştır. Her üç grupta MDA düzeyleri ölçülmüş ve apoptotik etkilerin saptanması amacıyla Kaspaz 3, Kaspaz 8, Kaspaz 9 ile p21 genlerinin ekspresyon düzeyleri RT-PCR yöntemi ile saptanmıştır. Bulgular: MTT testi ile uygun dozlar H2O2 için 40 μM, M. communis L. yağı için 15.625 μg/ml olarak saptanmıştır. Sağlıklı hücre grubuyla karşılaştırıldığında, 40 μM H2O2 maruziyeti uygulanan grupta MDA düzeyinde anlamlı artış gözlenirken (p=0,0005), M. communis L. esansiyel yağı ile tedavi edilen gruptaki MDA düzeyi kontrol grubu ile benzer düzeyde (p>0.05) bulunmuştur. 40 μM H2O2 uygulanan hücrelerde Kaspaz 3, Kaspaz 8 ve p21 genlerinin ekspresyon seviyelerinin sağlıklı hücre grubuna göre anlamlı bir şekilde arttığı (sırasıyla p=0.001, p=0.017 ve p=0,0003), Kaspaz 9 gen ekspresyon seviyesinin değişmediği (p=0.8) saptanmıştır. M. communis L. yağı ile tedavi edilen hücrelerde, oksidatif stres modelinin oluşturulduğu gruba göre Kaspaz 3 gen ekspresyon seviyesinin anlamlı şekilde azaldığı saptanmıştır (p=0.00007). Sonuç: Çalışmamızda, M. communis L. esansiyel yağının MDA seviyesini güçlü şekilde azaltıcı etkisi olduğu, ayrıca apopitozu durdurucu bir etki göstererek, teröpatik ajan olarak potansiyeli olduğu gösterildi. Bu bağlamda M. communis L. yağının güçlü bir antioksidan etkiye sahip olduğu, oksidatif stresten kaynaklanan apoptozisin durdurulmasında etkili olduğu düşünülmektedir.

🔖

Cite This Article

Ulutaş, H. Z., Duran, G. G. (2020). Antioxidant Effects of Myrtus communis L.’s Essential Oils in BEAS-2B Cells Induced by Oxidative Stress with Hydrogen Peroxide. Acta Medica Alanya, Cilt: 4 Sayı: 1, 21–28. https://doi.org/10.30565/medalanya.607989

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (30)

  1. Hayder N, Abdelwahed A, Kilani S, Ammar RB, Mahmoud A, Ghedira K, et al. Anti-genotoxic and free-radical scavenging activities of extracts from (Tunisian) Myrtus communis. Mutat Res Genet Toxicol Environ Mutagen. 2004;564(1):89-95. PMID:15474415
  2. Aydın C, Özcan MM. Determination of nutritional and physical properties of myrtle (Myrtus communis L.) fruits growing wild in Turkey. J Food Eng. 2007;79(2):453-8. DOI: 10.1016/j.jfoodeng.2006.02.008
  3. Mansouri S, Foroumadi A, Ghaneie T, Najar AG. Antibacterial Activity of the Crude Extracts and Fractionated Constituents of Myrtus communis. Pharmaceutical Bio. 2001;39(5):399-401. DOI: 10.1076/phbi.39.5.399.5889
  4. Cakir A. Essential oil and fatty acid composition of the fruits of Hippophae rhamnoides L. (Sea Buckthorn) and Myrtus communis L. from Turkey. Biochem Syst Ecol. 2004;32(9):809-16. DOI: 10.1016/j.bse.2003.11.010
  5. Gardeli C, Vassiliki P, Athanasios M, Kibouris T, Komaitis M. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts. Food Chem. 2008;107(3):1120-30. DOI: 10.1016/j.foodchem.2007.09.036
  6. Smith MA, Perry G, Pryor WA. Causes and consequences of oxidative stress in Alzheimer’s disease 1,2 1Guest Editors: Mark A. Smith and George Perry 2This article is part of a series of reviews on “Causes and Consequences of Oxidative Stress in Alzheimer’s Disease.” Free Radic Biol Med. 2002;32(11):1049. PMID:12031888
  7. Olinski R, Gackowski D, Foksinski M, Rozalski R, Roszkowski K, Jaruga P. Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis, and acquired immunodeficiency syndrome1. Free Radic Biol Med. 2002;33(2):192-200. DOI: 10.1016/S0891-5849(02)00878-X
  8. Mimica-Dukić N, Božin B, Soković M, Mihajlovic B, Matavulj M. Antimicrobial and Antioxidant Activities of Three Mentha Species Essential Oils. Planta Med. 2003;69(5):413-9. DOI: 10.1055/s-2003-39704
  9. Hayder N, Skandrani I, Kilani S, Bouhlel I, Abdelwahed A, Ben Ammar R, et al. Antimutagenic activity of Myrtus communis L. using the Salmonella microsome assay. S Afr J Bot.2008;74(1):121-5. DOI: 10.1016/j.sajb.2007.10.001
  10. Sushil JK. Membrane lipid peroxidation in erythrocytes of the newborn. Clin Chim Acta. 1986;161(3):301-6. DOI: 10.1016/0009-8981(86)90014-8
  11. Masoudi M, Miraj S, Rafieian-Kopaei M. Comparison of the Effects of Myrtus Communis L, Berberis Vulgaris and Metronidazole Vaginal Gel alone for the Treatment of Bacterial Vaginosis. J Clin Diagn Res. 2016;10(3):QC04-QC7. PMID: 27134945
  12. Kumar R, Phaneendra P, Bodhanapu S, Rahiman F, Mohamed NK, Tamizmani T. Antioxidant and hepatoprotective activity of the aqueous extract of Myrtus communis (myrtle) leaves. Pharmacololgy. 2011;1083-90.
  13. Rossi A, Di Paola R, Mazzon E, Genovese T, Caminiti R, Bramanti P, et al. Myrtucommulone from Myrtus communis Exhibits Potent Anti-Inflammatory Effectiveness in Vivo. J Pharmacol Exp Ther.2009;329(1):76-86. DOI: 10.1124/jpet.108.143214
  14. Mimika-Dukić N, Bugarin D, Grbović S, Mitić-Ćulafić D, Vuković-Gačić B, Orčić D, et al. Essential Oil of Myrtus communis L. as a Potential Antioxidant and Antimutagenic Agents. Molecules. 2010;15(4):2759-70. DOI: 10.3390/molecules15042759
  15. de Lavor ÉM, Fernandes AWC, de Andrade Teles RB, Leal AEBP, de Oliveira Júnior RG, Gama E Silva M, et al. Essential Oils and Their Major Compounds in the Treatment of Chronic Inflammation: A Review of Antioxidant Potential in Preclinical Studies and Molecular Mechanisms. Oxid Med Cell Longev. 2018;2018:6468593-. PMID:30671173
  16. Lu JM, Lin PH, Yao Q, Chen C. Chemical and molecular mechanisms of antioxidants: experimental approaches and model systems. J Cell Mol Med. 2010;14(4):840-60. PMID:19754673
  17. Dennis JM, Witting PK. Protective Role for Antioxidants in Acute Kidney Disease. Nutrients. 2017;9(7):718. DOI: 10.3390/nu9070718
  18. Park JE, Yang JH, Yoon SJ, Lee JH, Yang ES, Park JW. Lipid peroxidation-mediated cytotoxicity and DNA damage in U937 cells. Biochimie. 2002;84(12):1199-205. DOI: 10.1016/S0300-9084(02)00039-1
  19. Barrera G. Oxidative stress and lipid peroxidation products in cancer progression and therapy. ISRN Oncol. 2012;2012:137289. DOI: 10.5402/2012/137289
  20. Saha SK, Lee SB, Won J, Choi HY, Kim K, Yang G-M, et al. Correlation between Oxidative Stress, Nutrition, and Cancer Initiation. Int J Mol Sci. 2017;18(7):1544. DOI: 10.3390/ijms18071544
  21. Romani A, Coinu R, Carta S, Pinelli P, Galardi C, Vincieri FF, et al. Evaluation of antioxidant effect of different extracts of Myrtus communis L. Free Radic Res. 2004;38(1):97-103. DOI: 10.1080/10715760310001625609
  22. Ines S, Ines B, Wissem B, Mohamed BS, Nawel H, Dijoux-Franca MG, et al. In vitro antioxidant and antigenotoxic potentials of 3,5-O-di-galloylquinic acid extracted from Myrtus communis leaves and modulation of cell gene expression by H2O2. J Appl Toxicol. 2012;32(5):333-41. DOI: 10.1002/jat.1655
  23. Turhan N, Celik H, Duvan Cİ, Onaran Y, Aydın M, Armutcu F. Investigation of oxidative balance in patients with dysmenorrhea by multiple serum markers. J Turk Ger Gynecol Assoc. 2012;13(4):233-6. PMID: 24592048
  24. Fadaei B, Ahmadvand H, Ghasemi M, Mirzaie S. Inhibitory effects of myrtle (Myrtus communis L.) leaves hydroalcoholic extract on LDL oxidation in vitro. J Chem Pharm Res. 2015;42-6.
  25. Bajpai VK, Agrawal P, Bang BH, Park Y-H. Phytochemical analysis, antioxidant and antilipid peroxidation effects of a medicinal plant, Adhatoda vasica. Front Life Sci. 2015;8(3):305-12. DOI: 10.1080/21553769.2014.1002943
  26. Sahreen S, Khan MR, Khan RA. Comprehensive assessment of phenolics and antiradical potential of Rumex hastatus D. Don. roots. BMC Complement and Altern Med. 2014;14(1). DOI: 10.1186/1472-6882-14-47
  27. Miguel MG. Antioxidant activity of medicinal and aromatic plants. A review. Flavour Fragr J. 2010;25(5):291-312. DOI: 10.1002/ffj.1961
  28. Gonçalves S, Gomes D, Costa P, Romano A. The phenolic content and antioxidant activity of infusions from Mediterranean medicinal plants. Ind Crops Prod. 2013;43:465-71. DOI: 10.1016/j.indcrop.2012.07.066
  29. Fernald K, Kurokawa M. Evading apoptosis in cancer. Trends Cell Biol.2013;23(12):620-33. DOI: 10.1016/j.tcb.2013.07.006
  30. Tretiakova I, Blaesius D, Maxia L, Wesselborg S, Schulze-Osthoff K, Cinatl J, et al. Myrtucommulone from Myrtus communis induces apoptosis in cancer cells via the mitochondrial pathway involving caspase-9. Apoptosis. 2007;13(1):119-31. DOI: 10.1007/s10495-007-0150-0