Journals / Tekirdağ Ziraat Fakültesi Dergisi / 2026 / Cilt: 23 Sayı: 2

Determination of the Effective Mutation Dose Through Physical Mutagen Application in Pearl Millet (Pennisetum glaucum L. cv. Heavri) for the Mutation Breeding Program

Mutasyon Islahı Programına Yönelik Olarak İnci Darıda (Pennisetum glaucum L. cv. Heavri) Fiziksel Mutagen Uygulaması Sonucu Etkili Mutasyon Dozunun Belirlenmesi

Ali Şenay(Türkiye Enerji Nükleer ve Maden Araştırma Kurumu)0009-0002-3938-557X
Kadriye Yaprak Kantoglu(Türkiye Enerji Nükleer ve Maden Araştırma Kurumu, Nükleer Enerji Araştırma Enstitüsü)0000-0002-7247-9116
Irmak Çakın(Türkiye Enerji Nükleer ve Maden Araştırma Kurumu)0000-0001-8886-5348
Aslıhan Göktuğ(Türkiye Enerji Nükleer ve Maden Araştırma Kurumu)0009-0004-3646-5720
Aydın Ozan Çetintaş(Türkiye Enerji Nükleer ve Maden Araştırma Kurumu)0000-0002-4574-6573
Pages
375–383
Publication Date
2026-03-16
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Irradiation was conducted at doses of 150, 174, and 200 Gray to generate the M1 generation for the Heavri variety.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The problems caused by climate change have begun to significantly affect all areas of agricultural production.
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Key Findings & Data:In particular, the increase in temperature and the resulting drought have become major issues for crop species, leading to an increase in annual yield losses.
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Conclusion & Impact:Irradiation was conducted at doses of 150, 174, and 200 Gray to generate the M1 generation for the Heavri variety.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Pennisetum glaucum L. · Darı · Mutasyon ıslahı

Abstract

The problems caused by climate change have begun to significantly affect all areas of agricultural production. In particular, the increase in temperature and the resulting drought have become major issues for crop species, leading to an increase in annual yield losses. The advantages of millets (such as their nutritional value and high adaptability) are currently being considered in many countries as an alternative to crops that require more irrigation, especially due to the growing problem of drought. Various methods (classical breeding and biotechnological approaches) are being applied for millet breeding worldwide, and among these techniques, mutation breeding is one method that deserves particular attention. Countries that play an important role in millet production have initiated mutation breeding programs at the national and regional levels to increase nutrient content and enhance disease tolerance. In the present study, the effective mutation dose was determined as a preliminary step to initiate mutation breeding research for pearl millet (Pennisetum glaucum L. cv. Heavri), which has been agronomically examined in Türkiye. For this purpose, millet seeds with 8% moisture content were irradiated with cesium-137 gamma rays, a physical mutagenic source, at seven different doses (0, 50, 100, 200, 300, 400, and 500 Gray). As a result of the plant measurements taken three weeks after irradiation and the linear regression analysis, 174 Gy was determined to be the effective mutation dose. The negative effects of increasing gamma ray doses on plant development were clearly determined on both shoot length and fresh plant weight, and the resulting difference was determined to be statistically important. The 10% lower and upper limits of the effective mutation dose were defined. Irradiation was conducted at doses of 150, 174, and 200 Gray to generate the M1 generation for the Heavri variety.

Özet

İklim değişikliğinin yarattığı sorunlar tarımsal üretimin her alanında kendini önemli ölçüde hissettirmeye başlamıştır. Özellikle sıcaklık artışı ile beraberinde gelen kuraklık tarla bitkileri içinde yer alan türler için de önemli bir sorun oluşturmakta ve yıllık ürün kayıpları günden güne artmaktadır. Darı sahip olduğu avantajlar (besin değeri, yüksek adaptasyon kabiliyeti gibi) sonucunda bugün pek çok ülkede özellikle kuraklık sorunu nedeni ile son yıllarda sulama isteği fazla olan türlere alternatif olarak ele alınan bir türdür. Dünyada darı ıslahı konusunda farklı yöntemlerin (klasik ıslah ve biyoteknolojik yaklaşımlar) uygulaması söz konusu olup bu teknikler içinde yer alan mutasyon ıslahı tekniği üzerinde durulması gereken yöntemlerden bir tanesidir. Bugün darı üretiminde önemli yere sahip olan ülkeler bu amaçla gerek besin içeriğini artırmak gerekse hastalıklara tolerans sağlamak üzere mutasyon ıslahı programlarını ülkesel ve bölgesel düzeyde hayata geçirmiş durumdadır. Burada sonuçları sunulan araştırmada; ülkemizde üretimi yapılmak üzere agronomik gözlemleri tamamlanan inci darı (Pennisetum glaucum L. cv. Heavri) için mutasyon ıslahı çalışması başlatmak üzere ön aşama olan etkili mutasyon dozu belirlenmiştir. Bu amaçla yedi farklı dozda (0, 50, 100, 200, 300, 400, 500 Gray) fiziksel mutagen kaynağı olan sezyum 137 gama ışın kaynağı ile nem oranı %8 olan darı tohumları ışınlanmıştır. Işınlamadan üç hafta sonra yapılan bitkisel ölçümler ve lineer regresyon analizi sonucunda 174 Gray etkili mutasyon dozu olarak belirlenmiştir. Artan gama ışını dozlarının bitki gelişimine yönelik yaptığı olumsuz etki gerek sürgün uzunluğu gerekse yaş bitki ağırlığı üzerinde net bir şekilde belirlenerek istatistiki olarak ortaya çıkan farkın önemli olduğu saptanmıştır. Etkili mutasyon dozunun %10 alt ve üst sınırı belirlenerek 150, 174 ve 200 Gray dozlarında Heavri çeşidi için M1 generasyonunu oluşturulmak üzere ışınlama yapılmıştır.

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Cite This Article

Şenay, A., Kantoglu, K. Y., Çakın, I., Göktuğ, A., Çetintaş, A. O. (2026). Determination of the Effective Mutation Dose Through Physical Mutagen Application in Pearl Millet (Pennisetum glaucum L. cv. Heavri) for the Mutation Breeding Program. Tekirdağ Ziraat Fakültesi Dergisi, Cilt: 23 Sayı: 2, 375–383. https://doi.org/10.33462/jotaf.1532179

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References (40)

  1. Acar, R., Aslandağ, Y. and Osman, İ. M. (2022). Proso millet (Panicum miliaceum L) cultivation form and potentiality under konya conditions, Türkiye. Selcuk Journal of Agriculture and Food Science, 36(Special Issue): 83-85.
  2. Adhi, R. K., Qosim, W. A., Rachmadi, M. and Damayanti, F. (2024). Mutation breeding in underutilized cereal crops: a review. Bulgarian Journal of Agricultural Science, 30(1): 81-87.
  3. Ambavane, A. R., Sawardekar, S. V., Sawantdesai, S. A. and Gokhale, N. B. (2015). Studies on mutagenic effectiveness andefficiency of gamma rays and its effect on quantitative traits infinger millet (Eleusine coracana L. Gaertn). Journal of Radiation Research and Applied Sciences, 8: 120-125. https://doi.org/10.1016/j.jrras.2014.12.004
  4. Anonymous (2020). https://www.fssai.gov.in/upload/uploadfiles/files/Guidance_Notes_Version_2_Millets_29_01_2020.pdf (Accessed Date: 09.05.2024)
  5. Balkan, A., Bilgin, O., Başer, İ., Balaban Göçmen, D., Demirkan, A. K. and Deviren, B. (2019). Improvement of grain yield and yield associated traits in bread wheat (Triticum aestivum L.) genotypes through mutation breeding using gamma irradiation. Journal of Tekirdag Agricultural Faculty, 16(1): 103-111. https://doi.org/10.33462/jotaf.472456
  6. Benek, A. and Geren, H. (2023). A preliminary study on the influence of different N and P levels on the grain yield and some yield components of pearl millet (Pennisetum glaucum). Ispec Journal of Agricultural Sciences, 7(1): 36–45. https://doi.org/10.5281/Zenodo.7698823
  7. Bidinger, F. R. and Rai, K. N. (1989). Photoperiodic response of paternal lines and F1 hybrids in pearl millet. Indian Journal of Genetics and Plant Breeding, 49: 257-264.
  8. Bilgin, O., Sarıer, S. Y., Başer, İ. and Balkan, A. (2022). Enhancement of androgenesis and plant regeneration from wheat anther culture by seed pre-sowing gamma irradiation. Journal of Tekirdag Agricultural Faculty, 19(2): 354-365. https://doi.org/10.33462/jotaf.993270 (In Turkish)
  9. Çiçek, M. and Yücel, C. (2022). Determination of forage yield and quality of some pearl millet (Pennisetum glaucum (L.) R. Br.) populations in gap conditions. MAS JAPS, 7(Special Issue): 1143-1159. (In Turkish)
  10. Dağtekin, Z., Hatipoğlu, R. and Yücel, C. (2020). Agro-morphological and hay quality characteristics of some proso millet (Panicum miliaceaum L.) genotypes under Cukurova conditions. Turkish Journal of Nature and Science, 9(Special Issue): 84-91. https://doi.org/10.46810/tdfd.754974 (In Turkish)
  11. Dumanoğlu, Z., Özdemir, S. and Kökten, K. (2022). Determination of some morphological and physiological characteristics of seeds of different pearl millet (Pennisetum glaucum (L.) R. Br.) genotypes. ISPEC Journal of Agricultural Sciences, 6(1): 64-71. https://doi.org/10.46291/ISPECJASvol6iss1pp64-71
  12. Francis, N., Rajasekaran, R., Krishnamoorthy, I., Muthurajan, R., Thiyagarajan, C. and Alagarswamy, S. (2022a). Gamma irradiation to induce beneficial mutants in proso millet (Panicum miliaceum L.): an underutilized food crop. International Journal of Radiation Biology, 98(7): 1277-1288. https://doi.org/10.1080/09553002.2022.2024292
  13. Francis, N., Ravikesavan, R., Iyanar, K., Raveendran, M., Chitdeshwari, T. and Senthil, A. (2022b). Dose optimization, mutagenic effectiveness and efficiency of EMS in proso millet (Panicum miliaceum L.). Madras Agricultural Journal, 107: 10-12. https://doi.org/10.29321/MAJ.10.000575
  14. Ganapathy, K. N., Patro, T. S. S. K., Palanna, K. B., Das, I. K., Elangovan, M., Prashant, B., Venkatesha, S. C., Anuradha, N., Nagaraja, T. E. and Tonapi, V. A. (2021). Development of ımproved mutants for grain yield and related traits in finger millet (Eleusine Coracana L Gaertn.) through gamma mutagenesis. International Journal of Plant & Soil Science, 33(18):225-35. https://doi.org/10.9734/ijpss/2021/v33i1830592
  15. Gupta, P. K. and Yashvir. (1976). Induced mutations in foxtail millet (Setaria italica Beauv.). Theoretical and Applied Genetics, 48: 131–136. https://doi.org/10.1007/BF00281655
  16. Hanna, W. W. (1982). Mutation breeding of pearl millet and sorghum. Mutation Breeding Review, 1: 1-13.
  17. Hanna, W. W. and Young, J. R. (1974). Gamma radiation effects on pearl millet pollen, Crop Science, 14: 600-601. İstanbulluoğlu, A., Başer, İ. and Moray, S. (2023). Deficit irrigation programs effects on yield in millet (Panicum miliaceum L.) in Thrace Region conditions. KSÜ Journal of Agriculture and Nature, 26(2): 307-313. https://doi.org/10.18016/ksutarimdoga.vi.1007783
  18. Jency, J. P., Rajasekaran, R., Singh, R. K., Muthurajan, R., Prabhakaran, J., Mehanathan, M., Prasad, M. and Ganesan, J. (2020). Induced mutagenesis enhances lodging resistance and photosynthetic efficiency of kodomillet (Paspalum Scrobiculatum). Agronomy, 10: 227. https://doi.org/10.3390/agronomy10020227
  19. Kantoğlu, K. Y. and Kunter, B. (2021). Mutation Breeding. In: Ornamental Plant Breeding (Classical and Biotechnological Methods), Ed(s): Mendi N.Y. and Kazaz, S., Gece Publishing, Türkiye.
  20. Kate, S. M., Desai, S. S., Bhave, S. G., Thorat, B. S. and Bal, C. P. (2018). Mutagene induced variability in proso millet (Panicum miliaceum L.) International Journal of Chemical Studies, 6(5): 13-16.
  21. Kumar, B., Mahto, J. L. and Haider, Z. A. (1996). Induced mutation studies ın finger millet (Eleusine Coracana Gaertn.). Indian Journal of Genetics And Plant Breeding, 56(4): 526–532.
  22. Lee, D., Hanna, W., Buntin, G.D., Dozier, W., Timper, P. and Wilson, J.P. (2012). Pearl Millet for Grain. College of Agriculture and Environment Science, University of Georgia Cooperative Extension. Bulletin #B 1216. http://extension.uga.edu/publications/detail.cfm?number=B1216 (Accessed date: 30.05.2024).
  23. Mamo, T., Singh, A. K. and Mahama, A. A. (2023). Millet Breeding. In: Crop Improvement. Ed(s): Suza, W. P., and Lamkey, K. R. Iowa State University Digital Press, U. S. A.
  24. Mbinda, W. and Masaki, H. (2021). Breeding strategies and challenges in the improvement of blast disease resistance in finger millet. A Current Review. Frontiers in Plant Science, 11: 602882.
  25. MVD (2024). Mutant Variety Database. https://nucleus.iaea.org/sites/mvd/SitePages/Search.aspx (Accessed Date: 09.05.2024)
  26. Nakajo, S., Hasegawa, S., Yoshida, H., Urushibara S, Abe, A., Abe, T., Fukunishi, N. Ryutou, H. and Ohshimizu Y. (2013). Breeding new varieties of Japanese barnyard millet with shorter culms and low amylose content: “Nebarikko 1”, "Nebarikko 2”, and “Nebarikko 3”. Iwate Prefectural Agricultural Research Center Research Bulletin, 12: 43-60.
  27. Olak, H. and Tan, M. (2016). Effects of different nitrogen doses on yield and some traits in millet (Panicum italicum L.). Iğdır University Journal of the Institute of Science and Technology, 6(2):131-131. https://doi.org/10.21597/jist.2016218856 (In Turkish)
  28. Rajasekaran, R. and Francis, N. (2021). Genetic and genomic resources for improving proso millet (Panicum miliaceum L.): a potential crop for food and nutritional security. Nucleus, 64: 21-32. https://doi.org/10.1007/s13237-020-00331-2
  29. Ramesh, M., Vanniarajan, C., Ravikesavan, R., Eraivan Arutkani Aiyanathan, K. and Mahendran, P. P. (2019). Mutagenic effectiveness and efficiency in barnyard millet Echinochloa frumentacea using physical, chemical and combination of mutagens. Electronic Journal of Plant Breeding, 10(2): 949-956.
  30. Sağel, Z., Tutluer, İ., Peşkircioğlu, H., Kantoglu, Y., Tümbilen, Y., K., Kunter, and Özçoban, M. (2009). Yield, quantative characteristics and molecular determination of mutant TAEK-Sağel cultivar. Türkiye VIIth Field Crops Congress, 19-22 October. 1: 913-918, Hatay, Türkiye.
  31. Spencer-Lopes, M. M., Jankuloski, L., Mukhtar Ali Gahim, A., Matijevic, M., and Kodym, A. (2018). Physical Mutagenesis. In: Manual on Mutation Breeding Third Edition. Ed(s): Spencer-Lopes, M. M., Foster, B. P. and Jankuloski, L. FAO, IAEA Publishing.
  32. Sellapillai, L., Dhanarajan, A., Raina, A., and Ganesan, A. (2022). Gamma ray induced positive alterations in morphogenetic and yield attributing traits of finger millet (Eleusine coracana (L.) Gaertn.) in M2 generation. Plant Science Today, 9(4): 939–949. https://doi.org/10.14719/pst.1807
  33. Serba, D. D., Perumal, R., Tesso, T. T. and Min, D. (2017). Status of global pearl millet breeding programs and the way forward. Crop Science, 57: 2891-2905. https://doi.org/10.2135/cropsci2016.11.0936
  34. Serba, D. D., Yadav, R. S., Varshney, R. K., Gupta, S. K., Govindaraj, M., Srivastava, R., Gupta, R., Perumal, R. and Tesso T. T. (2019). Pearl Millet: A Resilient Crop for Arid and Semi-arid Environments. In: Genomic Designing of Climate-Smart Cereal Crops. Ed: Kole, C. Springer-Nature Press.
  35. Taylor, J. R, Schober, T. J. and Bean, S. (2006). Novel and non-food uses for sorghum and millets. Journal of Cereal Science, 44: 252-271.
  36. TUIK (2025). Turkish Statistical Institute. https://data.tuik.gov.tr/Bulten/Index?p=Bitkisel-Uretim-1.Tahmini-2025-53940 (Accessed Date: 29.09.2025)
  37. Van Harten, A. M. (1998). Mutation Breeding: Theory And Practical Applications. Cambridge University Press, U. K.
  38. Vijendra Das, L. D. (1978). Effects of radiation on the R and R1 progenies of Pennisetum typhoides. Environmental and Experimental Botany, 18(2): 121-125.
  39. Yadav, O. P., Gupta, S. K., Mahala, R. S., Ramalingam, A. P., Parray, S. A. and Perumal, R. (2024). Pearl Millet Hybrid Development and Seed Production. In: Pearl Millet. Ed(s): Perumal, R. Vara Prasad, P., Satyavathi, T., Govindaraj, M. and Tenkouano, A. American Society of Agronomy, Inc., Crop Science Society of America, Inc. and Soil Science Society of America, U.S.A. https://doi.org/10.1002/9780891184034.ch6
  40. Balkan, A., Bilgin, O., Başer, İ., Balaban Göçmen, D., Demirkan, A. K. and Deviren, B. (2019). Improvement of grain yield and yield associated traits in bread wheat (Triticum aestivum L.) genotypes through mutation breeding using gamma irradiation. Journal of Tekirdag Agricultural Faculty, 16(1): 103-111. https://doi.org/10.33462/jotaf.472456