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Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 4

Determination of the Seroprevalence of Leptospirosis in Cattle by MAT and ELISA in Hatay, Turkey

Özkan ASLANTAŞ
Pages
1019–1024
Publication Date
2005-04-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Statistical analyses indicated a significant difference in seroprevalence among locations (P < 0.05); however, no significant differences in positivity rates were found with respect to the age or sex of the animals (P > 0.05).

📌 3 Key Takeaways

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Objective & Hypothesis:Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars (grippotyphosa, hardjo and icterohaemorrhagie) using a microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA).
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Key Findings & Data:Forty-five (8.8%) and 72 (14%) of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively.
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Conclusion & Impact:Statistical analyses indicated a significant difference in seroprevalence among locations (P < 0.05); however, no significant differences in positivity rates were found with respect to the age or sex of the animals (P > 0.05).
🔬Design:Research Article
📍Scope:2003 Verileri
⚙️Variables:Leptospirosis · cattle · MAT

Abstract

Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars (grippotyphosa, hardjo and icterohaemorrhagie) using a microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). Forty-five (8.8%) and 72 (14%) of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively. Of the 45 MAT positive serum samples, 26 (57.7%) were positive against serovar grippotyphosa, 9 (20%) were positive against serovar hardjo, 1 (0.2%) was positive against serovar icterohaemorrhagie and 9 (20%) were positive against serovar grippotyphosa + serovar hardjo. Of the 72 ELISA positive serum samples, 32 (44.4%) were positive against serovar grippotyphosa, 15 (20.8%) were positive against serovar hardjo, 2 (2.8%) were positive against serovar icterohaemorrhagie and 23 (31.9%) were positive against serovar grippotyphosa + serovar hardjo. The dominant serovar was grippotyphosa. Statistical analyses indicated a significant difference in seroprevalence among locations (P < 0.05); however, no significant differences in positivity rates were found with respect to the age or sex of the animals (P > 0.05).

Özet

Five hundred and twelve serum samples collected from randomly selected cattle of different age and sex from the towns and central villages of Hatay during April-July 2003 were tested for antibodies against 3 different Leptospira interrogans serovars (grippotyphosa, hardjo and icterohaemorrhagie) using a microscopic agglutination test (MAT) and enzyme-linked immunosorbent assay (ELISA). Forty-five (8.8%) and 72 (14%) of the 512 serum samples tested were detected to be positive with MAT and ELISA, respectively. Of the 45 MAT positive serum samples, 26 (57.7%) were positive against serovar grippotyphosa, 9 (20%) were positive against serovar hardjo, 1 (0.2%) was positive against serovar icterohaemorrhagie and 9 (20%) were positive against serovar grippotyphosa + serovar hardjo. Of the 72 ELISA positive serum samples, 32 (44.4%) were positive against serovar grippotyphosa, 15 (20.8%) were positive against serovar hardjo, 2 (2.8%) were positive against serovar icterohaemorrhagie and 23 (31.9%) were positive against serovar grippotyphosa + serovar hardjo. The dominant serovar was grippotyphosa. Statistical analyses indicated a significant difference in seroprevalence among locations (P < 0.05); however, no significant differences in positivity rates were found with respect to the age or sex of the animals (P > 0.05).

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Cite This Article

ASLANTAŞ, Ö. (2005). Determination of the Seroprevalence of Leptospirosis in Cattle by MAT and ELISA in Hatay, Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 4, 1019–1024. https://ajindex.com/articles/133038

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References (11)

  1. Çetinkaya, B., Ertafl, H.B., Muz, A., Öngör, H., Kalender, H., Özdemir, V.: Determination of seroprevalence of leptospirosis in cattle in Elazığ. Turk. J. Vet. Anim. Sci., 1999; 23: 633-639.
  2. Bulu, A.A., Dörterler, R., Özkan, Ö., Hofltürk, F.: Doğu Anadolu’nun bazı illerinde (Kars, Artvin, Gümüflhane, Erzurum) sığır ve koyunlarda leptospirosis vakaları, yayılıflı ve serotipleri üzerine arafltırma. Etlik Vet. Mikrobiyol. Ens. Derg., 1990; 6: 49- 60. Özdemir, V., Kaya, K.: Türkiye genelinde sığır leptospira serotiplerinin dağılımının saptanması ve leptospirozise karflı bir aflı denemesi üzerine çalıflmalar. Etlik Vet. Mikrobiyol. Derg., 2000; 11: 11-21. Anonymous: Office International des Epizooties. Word Organisation for animal health. Manual of standards for diagnostic tests and vaccines. 3 rd ed., ISBN, 92-9044-423-1, 1996.
  3. Ribotta, M.J., Higgins, R., Gottschalk, M., Lallier, R.: Development of an indirect enzyme-linked immunosorbent assay for the detection of leptospiral antibodies in dogs. Can. J. Vet. Res., 2000; 64: 32–37.
  4. Bercovich, Z., Taaijke, R., Bokhout, B.A.: Evaluation of an ELISA for the diagnosis of experimentally induced and naturally occurring Leptospira hardjo infections in cattle. Vet. Microbiol., 1990; 21: 255-262.
  5. Cousins, D.V., Robertson, G.M., Hustas, L.: The use of the enzyme-linked immunosorbent assay (ELISA) to detect the IgM and IgG antibody response to Leptospira interrogans serovars hardjo, pomona, and tarassovi in cattle. Vet. Microbiol., 1983; 10: 439-450.
  6. Ramadass, P., Samuel, B., Nachimuthu, K.: A rapid latex agglutination test for the detection of leptospiral antibodies. Vet. Microbiol., 1999; 70: 137–140.
  7. Çetinkaya, B., Ertafl, H.B., Öngör, H., Muz, A.: Detection of leptospira species by polymerase chain reaction (PCR) in urine of cattle. Turk. J. Vet. Anim. Sci., 2000; 24: 123–130.
  8. Van Eys, G.J.J.M., Gravekamp, C., Gerritsen, M.J., Quint, W., Cornelissen, T.E., Ter Schegget, J., Terpstra, W.J.: Detection of leptospires in urine by polymerase chain reaction. J. Clin. Microbiol., 1989; 27: 2258-2262.
  9. Merien, F., Amouriaux, P., Perolat, P., Baranton, G., Saint Girons, I.: Polymerase chain reaction for the detection of Leptospira spp. in clinical samples. J. Clin. Microbiol., 1992; 30: 2219-2224.
  10. Faine, S.: Guidelines for the control of leptospirosis. World Health Organisation. Geneva. 1982.
  11. Terpstra, W.J., Ligthart, G.S., Schoone G.J.: Serodiagnosis of human leptospirosis by enzyme-linked-immunosorbent-assay (ELISA). Zentral