Journals / Turkish Journal of Veterinary and Animal Sciences / 2005 / Cilt: 29 - Sayı: 4

Isolation of Listeria monocytogenes from Fish Intestines and RAPD Analysis

Pages
1007–1011
Publication Date
2005-04-01
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results indicated that the isolates might have originated from different sources, and fish contaminated with L. monocytogenes may cause serious health problems in humans.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:One hundred and fifty fish caught from Keban Dam Lake were examined for the presence of Listeria monocytogenes.
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Key Findings & Data:Ten (6.6%) L. monocytogenes were isolated and identified from fish intestines.
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Conclusion & Impact:The results indicated that the isolates might have originated from different sources, and fish contaminated with L. monocytogenes may cause serious health problems in humans.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Fish · intestine · Listeria monocytogenes

Abstract

One hundred and fifty fish caught from Keban Dam Lake were examined for the presence of Listeria monocytogenes. A random amplified polymorphic DNA (RAPD) assay using a random primer was performed to detect genetic variations among L. monocytogenes isolates. The selective enrichment procedure followed by selective isolation was used for listeria isolation from fish intestines. Ten (6.6%) L. monocytogenes were isolated and identified from fish intestines. Two different band profiles were obtained by the RAPD analysis of the isolates. The results indicated that the isolates might have originated from different sources, and fish contaminated with L. monocytogenes may cause serious health problems in humans.

Özet

One hundred and fifty fish caught from Keban Dam Lake were examined for the presence of Listeria monocytogenes. A random amplified polymorphic DNA (RAPD) assay using a random primer was performed to detect genetic variations among L. monocytogenes isolates. The selective enrichment procedure followed by selective isolation was used for listeria isolation from fish intestines. Ten (6.6%) L. monocytogenes were isolated and identified from fish intestines. Two different band profiles were obtained by the RAPD analysis of the isolates. The results indicated that the isolates might have originated from different sources, and fish contaminated with L. monocytogenes may cause serious health problems in humans.

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Cite This Article

ERTAŞ, H. B., ŞEKER, E. (2005). Isolation of Listeria monocytogenes from Fish Intestines and RAPD Analysis. Turkish Journal of Veterinary and Animal Sciences, Cilt: 29 - Sayı: 4, 1007–1011. https://ajindex.com/articles/133036

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