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Journals / Turkish Journal of Veterinary and Animal Sciences / 2006 / Cilt: 30 - Sayı: 3

Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs

Youjin TU
Chunlan XU
Yizhen WANG
Guojia YAO
Tizhong SHAN
Pages
325–330
Publication Date
2006-03-01
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression.

📌 3 Key Takeaways

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Objective & Hypothesis:This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide (APS) on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis.
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Key Findings & Data:One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets (21.88 ± 1.26 kg of average initial body weight) were randomly allotted to 4 treatments.
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Conclusion & Impact:The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Astragalus · PR-39 · protegrin-1

Abstract

This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide (APS) on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis. One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets (21.88 ± 1.26 kg of average initial body weight) were randomly allotted to 4 treatments. Each treatment had 3 replicates (i.e. pens) with 10 pigs per pen. The basal diets were supplemented with 0, 5 g kg-1 APS, 5 g kg-1 80 mesh Astragalus, and 5 g kg-1 micron Astragalus, respectively, and the feeding experiment lasted 30 days. After the feeding experiment, 12 pigs with 3 animals in each treatment were chosen to determine the effect of Astragalus or APS on PR-39 and protegrin-1 mRNA expression of pigs. The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression. Pigs fed the diet containing 5 g kg-1 80 mesh Astragalus also had higher PR-39 and protegrin-1 gene expression level than did the control (P < 0.05). However, micron Astragalus was more effective than 80 mesh Astragalus in enhancing PR-39 and protegrin-1 gene expression. Supplementation with 5 g kg-1 APS reduced PR-39 and protegrin-1 gene expression (P < 0.05).

Özet

This experiment was conducted to study the effects of micron Astragalus, 80 mesh Astragalus and Astragalus polysaccharide (APS) on antimicrobial peptides PR-39 and protegrin-1 gene expression in pigs using semi-quantitative RT-PCR analysis. One hundred twenty 60-day-old Duroc x Landrace x Yorkshire piglets (21.88 ± 1.26 kg of average initial body weight) were randomly allotted to 4 treatments. Each treatment had 3 replicates (i.e. pens) with 10 pigs per pen. The basal diets were supplemented with 0, 5 g kg-1 APS, 5 g kg-1 80 mesh Astragalus, and 5 g kg-1 micron Astragalus, respectively, and the feeding experiment lasted 30 days. After the feeding experiment, 12 pigs with 3 animals in each treatment were chosen to determine the effect of Astragalus or APS on PR-39 and protegrin-1 mRNA expression of pigs. The results showed that supplementation with 5 g kg-1 micron Astragalus significantly (P < 0.05) enhanced antimicrobial peptides PR-39 and protegrin-1 gene expression. Pigs fed the diet containing 5 g kg-1 80 mesh Astragalus also had higher PR-39 and protegrin-1 gene expression level than did the control (P < 0.05). However, micron Astragalus was more effective than 80 mesh Astragalus in enhancing PR-39 and protegrin-1 gene expression. Supplementation with 5 g kg-1 APS reduced PR-39 and protegrin-1 gene expression (P < 0.05).

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Cite This Article

TU, Y., XU, C., WANG, Y., YAO, G., SHAN, T. (2006). Effect of Astragalus and Astragalus Polysaccharide on Antimicrobial Peptides PR-39 and Protegrin-1 Gene Expression in Pigs. Turkish Journal of Veterinary and Animal Sciences, Cilt: 30 - Sayı: 3, 325–330. https://ajindex.com/articles/133021

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