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Journals / Turkish Journal of Veterinary and Animal Sciences / 2006 / Cilt: 30 - Sayı: 3

Identification of Vibrio anguillarum by PCR (rpoN Gene) Associated with Vibriosis in Marine Fish in Turkey

Didem DEMİRCAN
Akın CANDAN
Pages
305–310
Publication Date
2006-03-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, this gene could be used in the diagnosis of V. anguillarum strains isolated in Turkey.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The main causative agent of vibriosis is Vibrio anguillarum.
📊
Key Findings & Data:In this study, 33 V. anguillarum strains were isolated from the internal organs of marine fish showing typical clinical signs of vibriosis, and these strains were identified by biochemical tests.
💡
Conclusion & Impact:In conclusion, this gene could be used in the diagnosis of V. anguillarum strains isolated in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Vibrio anguillarum · PCR · rpoN

Abstract

The main causative agent of vibriosis is Vibrio anguillarum. In this study, 33 V. anguillarum strains were isolated from the internal organs of marine fish showing typical clinical signs of vibriosis, and these strains were identified by biochemical tests. For comparison of these results, API 20E and BIONOR Mono-Va agglutination kits were used. Finally strains were amplified by PCR using the rpoN gene. Although the phenotypic properties of V. anguillarum strains varied, the rpoN gene from chromosomal DNA was amplified in all strains. In conclusion, this gene could be used in the diagnosis of V. anguillarum strains isolated in Turkey.

Özet

The main causative agent of vibriosis is Vibrio anguillarum. In this study, 33 V. anguillarum strains were isolated from the internal organs of marine fish showing typical clinical signs of vibriosis, and these strains were identified by biochemical tests. For comparison of these results, API 20E and BIONOR Mono-Va agglutination kits were used. Finally strains were amplified by PCR using the rpoN gene. Although the phenotypic properties of V. anguillarum strains varied, the rpoN gene from chromosomal DNA was amplified in all strains. In conclusion, this gene could be used in the diagnosis of V. anguillarum strains isolated in Turkey.

🔖

Cite This Article

DEMİRCAN, D., CANDAN, A. (2006). Identification of Vibrio anguillarum by PCR (rpoN Gene) Associated with Vibriosis in Marine Fish in Turkey. Turkish Journal of Veterinary and Animal Sciences, Cilt: 30 - Sayı: 3, 305–310. https://ajindex.com/articles/132349

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