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Journals / Türk Tarım ve Doğa Bilimleri Dergisi / 2026 / Volume: 12 Issue: 2

Identification of the Parasitoid Wasp Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae) in Pistachio Orchards Using DNA Barcoding

Siirt Fıstığı Bahçelerinde Coccinellidae Türlerinin Parazitoidi Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae)’nın DNA Barkodlama ile Tanımlanması

Halil Dilmen(SİİRT ÜNİVERSİTESİ)0000-0002-3802-9947
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Publication Date
2025-04-16
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The phylogenetic tree indicated that D. coccinellae is most closely related to Meteorus obfuscatus, with a genetic distance of 0.10, providing insights into interspecies relationships.

📌 3 Key Takeaways

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Objective & Hypothesis:Identification of Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae), a parasitoid of various species within the Coccinellidae family, is essential for the development of effective biological control strategies in pistachio orchards.
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Key Findings & Data:This study aimed to accurately identify D. coccinellae utilizing DNA barcoding techniques.
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Conclusion & Impact:The phylogenetic tree indicated that D. coccinellae is most closely related to Meteorus obfuscatus, with a genetic distance of 0.10, providing insights into interspecies relationships.
🔬Design:Research Article
📍Scope:2023 Verileri
⚙️Variables:Dinocampus coccinellae · Coccinellidae · DNA barcoding

Abstract

Identification of Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae), a parasitoid of various species within the Coccinellidae family, is essential for the development of effective biological control strategies in pistachio orchards. This study aimed to accurately identify D. coccinellae utilizing DNA barcoding techniques. Research was conducted throughout 2023 on coccinellid specimens collected regularly from pistachio orchards in Siirt. Among the collected Coccinellidae species, D. coccinellae DNA was successfully identified in the Hippodamia variegata specimen among a total of 11 individuals. The mitochondrial COI gene region of D. coccinellae was analyzed by DNA barcoding analysis using Polymerase Chain Reaction (PCR) and sequencing methods. PCR results and DNA sequencing confirmed the accurate identification of D. coccinellae, demonstrating high similarity with reference sequences in the NCBI database. The sequencing results exhibited high accuracy and reliability, with a similarity rate of 97.13% and an E-value of 0.0. Phylogenetic relationships were assessed using the Maximum Likelihood (ML) method. Phylogenetic analyses clearly illustrated the relationships of D. coccinellae with other Hymenoptera species. The phylogenetic tree indicated that D. coccinellae is most closely related to Meteorus obfuscatus, with a genetic distance of 0.10, providing insights into interspecies relationships.

Özet

Siirt fıstığı bahçelerinde, Coccinellidae familyasından çeşitli türlerin parazitoidi olan Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae)'nın tanımlanması, etkili biyolojik kontrol stratejilerinin geliştirilmesi açısından kritik öneme sahiptir. Bu çalışma, D. coccinellae’'nin DNA barkodlama yöntemi kullanılarak kesin tanımlanmasını amaçlamıştır. Araştırma, 2023 yılı boyunca Siirt fıstığı bahçelerinden düzenli olarak toplanan coccinelid örnekler üzerinde gerçekleştirilmiştir. Çalışma sonucunda, toplanan Coccinellidae türleri arasında, toplam 11 birey içinde Hippodamia variegata örneği üzerinde D. coccinellae DNA'sı doğru bir şekilde tespit edilmiştir. Dinocampus coccinellae'nin mitokondrial COI gen bölgesi, Polimeraz Zincir Reaksiyonu (PCR) ve dizileme yöntemleri kullanılarak DNA barkodlama analizi ile incelenmiştir. PCR sonuçları ve DNA dizileme analizi, D. coccinellae'nin doğru bir şekilde tanımlandığını ve yüksek bir benzerlik oranıyla NCBI veritabanındaki referans dizilerle uyumlu olduğunu ortaya koymuştur. Dizileme sonuçları, %97.13'lük bir benzerlik oranı ve 0.0 E-değeri ile yüksek doğruluk ve güvenilirlik sağlamıştır. Filogenetik ilişkilerin belirlenmesinde Maximum Likelihood (ML) yöntemi kullanılmıştır. Filogenetik analizler, D. coccinellae’nın diğer Hymenoptera türleriyle olan akrabalık ilişkilerini net bir şekilde göstermiştir. Filogenetik ağaç, D. coccinellae’nın en yakın akrabası olarak Meteorus obfuscatus ile 0.08 genetik uzaklığa sahip olduğunu ortaya koymuş, bu da türler arasındaki akrabalık ilişkileri hakkında bilgiler sunmuştur.

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Cite This Article

Dilmen, H. (2025). Identification of the Parasitoid Wasp Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae) in Pistachio Orchards Using DNA Barcoding. Türk Tarım ve Doğa Bilimleri Dergisi, Volume: 12 Issue: 2. https://doi.org/10.30910/turkjans.1531632

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