Journals / Tavukçuluk Araştırma Dergisi / 2026 / Volume: 23 Issue: 1

Synergistic and Individual Effects of Licorice Root and Plantain Herb Extracts Supplemented Through Drinking Water on Performance and Egg Quality in Laying Hens

Ezgi Şenses(Department of Animal Science, Faculty of Agriculture, Bursa Uludag University)0000-0003-3707-6415
Berkay Erdoğmuş(Department of Animal Science, Faculty of Agriculture)2000-0900-0616-6143
Arda Çelik(Department of Animal Science, Faculty of Agriculture)0000-0002-0955-4371
Pages
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Publication Date
2026-06-08
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, licorice root extract positively affected the egg production and quality, whereas plantain herb extract mainly improved shell strength.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study examined the effects of licorice root (Glycyrrhiza glabra) and plantain herb (Plantago major) extracts, individually or combined, in drinking water on laying performance and egg quality in laying hens.
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Key Findings & Data:A total of 280 Lohmann Brown hens (30 weeks old) were assigned to four groups: (1) control (no extract), (2) licorice root extract (3 ml/l), (3) plantain herb extract (3 ml/l), and (4) licorice root + plantain herb extract (1.5 + 1.5 ml/l).
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Conclusion & Impact:Overall, licorice root extract positively affected the egg production and quality, whereas plantain herb extract mainly improved shell strength.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Plant extracts · Plantain extract · Licorice root

Abstract

This study examined the effects of licorice root (Glycyrrhiza glabra) and plantain herb (Plantago major) extracts, individually or combined, in drinking water on laying performance and egg quality in laying hens. A total of 280 Lohmann Brown hens (30 weeks old) were assigned to four groups: (1) control (no extract), (2) licorice root extract (3 ml/l), (3) plantain herb extract (3 ml/l), and (4) licorice root + plantain herb extract (1.5 + 1.5 ml/l). Licorice root extract supplementation resulted in a significantly higher egg weight compared to the control group (P < 0.05). Egg production was also improved in both the licorice root and plantain herb groups relative to the other treatments. Similarly, egg mass and feed efficiency were significantly better in hens receiving licorice root and plantain herb extracts compared to the control (P < 0.05). Regarding egg quality, licorice root extract improved the Haugh unit, while plantain herb extracts enhanced shell quality, both separately and in combination. Overall, licorice root extract positively affected the egg production and quality, whereas plantain herb extract mainly improved shell strength. These findings suggest that supplementation of licorice root or plantain herb extract into drinking water may enhance the performance and egg quality of laying hens.

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Cite This Article

Şenses, E., Erdoğmuş, B., Gümüş, İ. H., Çelik, A. (2026). Synergistic and Individual Effects of Licorice Root and Plantain Herb Extracts Supplemented Through Drinking Water on Performance and Egg Quality in Laying Hens. Tavukçuluk Araştırma Dergisi, Volume: 23 Issue: 1. https://doi.org/10.34233/jpr.1929319

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References (52)

  1. Aghdam, S. H., Ahmadzadeh, A., Nobakht, A. (2018). Effects of different levels of Licorice (Glycyrrhiza glabra) medicinal plant powder on performance, egg quality and some of serum biochemical parameters in laying hens.
  2. Akbaş, Y., Altan, Ö., Koçak, Ç. (1996). Effects of hen age on the internal and external quality characteristics of chicken eggs Turkish Veterinary Journal, 20, 445-460.
  3. Alagawany, M., Elnesr, S. S., Farag, M. R., Abd El-Hack, M. E., Khafaga, A. F., Taha, A. E., Dhama, K. (2019). Use of licorice (Glycyrrhiza glabra) herb as a feed additive in poultry: Current knowledge and prospects. Animals, 9(8), 536. https://doi.org/10.3390/ani9080536
  4. Al-Daraji, H. J., H. A. Al-Saraf (2006). Effect of using high levels of licorice extract from the first day of chicks' age on productive performance of broiler chickens. Iraqi Poultry Science Journal, 1(1), 38-52. https://www.iasj.net/iasj/article/59541
  5. Al-Daraji, H. J., W. K. Al-Hayani, H. A. Al-Mashadani., D. H. Al-Hassani (2009). The use of different concentrations of licorice extract (Glycyrrhiza glabra) for improving egg quality traits of Lohmann hens reared during different periods of the year. Alfurat Journal of Agricultural Sciences, 1(2): 71–80.
  6. Al-Sofee, K. H. M. (2018). Effect of different levels of licorice roots powder to diets on productive performance and some blood traits for quail. Mesopotamia Journal of Agriculture, 46(4), 135-144.
  7. Awadein, N. B., Eid, Y. Z., Abd El-Ghany, F. A. (2010). Effect of dietary supplementation with phytoestrogens sources before sexual maturity on productive performance of mandarah hens. Poultry Science Journal, 30(3), 829-846.
  8. Bahadori, M. B., Sarikurkcu, C., Kocak, M. S., Calapoglu, M., Uren, M. C., Ceylan, O. (2020). Plantago lanceolata as a source of health-beneficial phytochemicals: Phenolics profile and antioxidant capacity. Food Bioscience, 34, 100536. https://doi.org/10.1016/j.fbio.2020.100536
  9. Beara, I. N., Lesjak, M. M., Jovin, E. Đ., Balog, K. J., Anackov, G. T., Orcic, D. Z., & Mimica-Dukic, N. M. (2009). Plantain (Plantago L.) species as novel sources of flavonoid antioxidants. Journal of Agricultural and Food Chemistry, 57(19), 9268-9273.
  10. Beara, I.N., Lesjak, M.M., Orcic, D.Z., Simin, N.D., Cetojevic-Simin, D.D., Bozin, B.N., and amp; Mimica-Dukic, N.M. (2012). Comparative analysis of phenolic profile, antioxidant, anti-inflammatory and cytotoxic activity of two closely-related Plantain species: Plantago altissima L. and Plantago lanceolata L. LWT-Food Science and Technology, 47, 64-70. https://doi.org/10.1016/j.lwt.2012.01.001
  11. Beardsworth, P.M., Hernandez, J.M. (2004). Yolk colour-an important egg quality attribute. International Poultry Production. 12(5): 17-18.
  12. Butaye P, Van Damme K, Devriese LA, Van Damme L, Bael M, Lauwers S, Haesebrouck F. (2000). In vitro susceptibility of Enterococcus faecium isolated from food to growth-promoting and therapeutic antibiotics. International Journal of Food Microbiology, 54, 181–187. https://doi.org/10.1016/S0168-1605(99)00198-1
  13. Çabuk, M., Alçiçek, A., Bozkurt, M. ve İmre, E. (2003). Antimicrobial properties of essential oils obtained from aromatic plants and their potential use as alternative feed. II. Ulusal Hayvan Besleme Kongresi Bildiri Kitabı (ss. 184-187), Selçuk Üniversitesi Ziraat Fakültesi, Konya, Türkiye, 18–20 Eylül.
  14. Dalkılıç, B., Güler, T., Ertaş, O.N., Çiftci, M. (2005). The Effect Of Thyme And Anise Oils And Antibiotic on Total Cecum Coliform Bacteria III. Ulusal Hayvan Besleme Kongresi Bildirileri, Çukurova Üniversitesi Ziraat Fakültesi, Adana, 7–10 Eylül.
  15. Dogan, S. C., Erdoğan, Z., Şekeroğlu, A., Baylan, M., & Küçükgül, A. (2018a). The effects of licorice root powder (Glycyrrhriza glabra) on performance, serum parameters, egg yolk cholesterol and antioxidant capacity of laying japanese quail. Turkish Journal of Agriculture-Food Science and Technology, 6(9), 1290-1296. DOI: https://doi.org/10.24925/turjaf.v6i9.1290-1296.2124
  16. Dogan, S. C., Baylan, M., Erdoğan, Z., Küçükgül, A., & Bulancak, A. (2018b). The effects of Licorice (Glycyrrhriza glabra) root on performance, some serum parameters and antioxidant capacity of laying hens. Brazilian Journal of Poultry Science, 20, 699-706. https://doi.org/10.1590/1806-9061-2018-0767
  17. Dogan, S. C., Baylan, M., Yaman, S., & Bulancak, A. (2018c). Effects of dietary licorice root (Glycyrrhriza glabra) supplementation, storage time and temperature on quality of quail eggs. Progress in Nutrition, 20(4), 665-671. DOI: 10.23751/pn.v20i4.7854
  18. Dorhoi, A., Dobrean, V., Zăhan, M., Virag, P. (2006). Modulatory effects of several herbal extracts on avian peripheral blood cell immune responses. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 20(5), 352-358. https://doi.org/10.1002/ptr.1859
  19. Dosoky, W. M., Zeweil, H. S., Ahmed, M. H., Zahran, S. M., Ali, A. M., Abdelsalam, N. R., & Naiel, M. A. (2021). The influences of Tylosine and licorice dietary supplementation in terms of the productive performance, serum parameters, egg yolk lipid profile, antioxidant and immunity status of laying Japanese quail under heat stress condition. Journal of Thermal Biology, 99, 103015. https://doi.org/10.1016/j.jtherbio.2021.103015
  20. Ehtesham, A., Chowdhury, S.D., (2002). Responses of laying hens to diets formulated by using different feeding standards. Pakistan Journal of Nutrition. 1, 127-131. DOI: https://doi.org/10.3923/pjn.2002.127.131
  21. Froning, G. W., Funk, E. M. (1958). Seasonal variation in quality of eggs laid by caged layers and their sisters on the floor. Poultry Science, 37(1), 215-223. https://doi.org/10.3382/ps.0370215
  22. Galib A.A.K., Hasan S.A.J., Nihad A.L.A., and amp; Ihsan H.A.T. (2023). Protein replacers in poultry diets. Revis Bionatura, 8 (2), 78.
  23. Ghasemi, R., Zarei, M., Torki, M. (2010). Adding medicinal herbs including garlic (Allium sativum) and thyme (Thymus vulgaris) to diet of laying hens and evaluating productive performance and egg quality characteristics. American Journal of Animal and Veterinary Sciences, 5(2), 151-154.
  24. Gholami-Ahangaran M, Ahmadi-Dastgerdi A, Azizi S, Basiratpour A, Zokaei M, Derakhshan M. (2022). Thymol and carvacrol supplementation in poultry health and performance. Veterinary Medicine and Science 8(1): 267–288. https://doi.org/10.1002/vms3.663
  25. Gupta, V. K., Fatima, A., Faridi, U., Negi, A. S., Shanker, K., Kumar, J. K., Khanuja, S. P. (2008). Antimicrobial potential of Glycyrrhiza glabra roots. Journal of Ethnopharmacology, 116(2), 377-380. https://doi.org/10.1016/j.jep.2007.11.037
  26. Hanafy, M. M. (2022). The impact of stocking density and licorice powder spplementation on the productive state and some physiological traits of laying hens. Egyptian Poultry Science Journal, 42(1), 17-38. https://doi.org/10.21608/EPSJ.2022.225211
  27. Haugh, R. R., (1937). The Haugh unit for measuring egg quality, US Poultry Magazine, 43,552-555, 572-573. https://doi.org/10.24925/turjaf.v6i9.1290-1296.2124
  28. Işık, Y., Babacan, O., Taş, T. (2025). Use of medicinal and aromatic plants in poultry nutrition. Düzce Üniversitesi Ziraat Fakültesi Dergisi, 3(1), 54-66. Moore, G., Sanford, P., Wiley, T. (2006). Perennial pastures for Western Australia (Bulletin 4690). Perth: Department of Agriculture and Food.
  29. O’Neill J. (2016). Review on Antimicrobial Resistance: Tackling Drug-Resistant Infections Globally: Final Report and Recommendations. Available. Accessed date: 09.09.2024
  30. Ody, P., (1993). The Complete Medicinal Herbal. New York, Dorling Kindersley Limited, 70: 138-139.
  31. Perera, W. N. U., Ravindran, V. (2025). Role of feed additives in poultry nutrition: Historical, current and future perspectives. Animal Feed Science and Technology, 116371. https://doi.org/10.1016/j.anifeedsci.2025.116371
  32. Połtowicz K., Wężyk S. (2001). Wpływ dodatku ziół w żywieniu kur nieśnych na produkcyjność i jakość jaj. Roczniki Naukowe Zootechniki, 28, 2: 215-225.
  33. Rauch W. (1958). Verglerchende Untersuchungen Zur Qualitats Beurteilung Von Frischeiem. Archives für Geflugelkunde, 22: 74-104.
  34. Reddy, P. M., Reddy, V. R., Reddy, C. V., Rao, P. S. P. (1981). Egg weight, shape index and hatchability in Khaki Campbell duck eggs.
  35. Redoy, M.R.A., Rahman, M.A., Atikuzzaman, M., Shuvo, A.A.S., Hossain, E., Khan, M.J., and amp; Al-Mamun, M. (2021). Dose titration of plantain herb (Plantago lanceolata L.) supplementation on growth performance, serum antioxidants status, liver enzymatic activity and meat quality in broiler chickens. Italian Journal of Animal Science. 20(1), 1244-1255. https://doi.org/10.1080/1828051X.2021.1952114
  36. Rezaei M, Kalantar Nasr J. (2014). Thymus vulgaris L., Glycyrrhiza glabra, and combo enzyme in corn or barley-basal diets in broiler chickens. International Journal of Plant, Animal and Environmental Science; 4(3):418-423.
  37. Safari A, Zahedi A. (2016). The effect of different levels supplementation of Glycyrrhiza glabraextract on growth performance in male quail. International Journal of Advanced Biological and Biomedical Research; 4(2):214-219.
  38. Salary J, Kalantar M, Sahebi ala MK. Ranjbar K, Hemati Matin HR. (2014). Drinking water supplementation of licorice and aloe vera extracts in broiler chickens. Scientific Journal of Animal Science; 3(2):41-48. doi: 10.14196/sjas.v3i2.1144
  39. Samuelsen AB. (2000). The traditional uses, chemical constituents and biological activities of Plantago major L. A review. Journal of Ethnopharmacology. Jul;71(1-2):1-21.
  40. Sedghi M, Golian A, Kermanshahi H, Ahmadi H. (2010a). Effect of dietary supplementation of licorice extract and a prebiotic on performance and blood metabolites of broilers. South African Journal of Animal Science; 40(4):371-380. DOI:10.4314/sajas.v40i4.65259
  41. Sedghi, M., Golian, A.G.H. and Soleymani, P. (2010b). Effect of dietary supplementation of licorice extract on egg quality and performance of hens. Veterinary Clininal Pathology 4(15), 933-941.
  42. Selaledi, A.L., Hassan, M.Z., Manyelo, T.G., & Mabelebele, M. (2020). The current status of the alternative use to antibiotics in poultry production: an African perspective. Antibiotics, 9(9),594612. https://doi.org/10.3390/antibiotics9090594
  43. Sharp, P. F., and C. K. Powell, (1930). Decrease in internal quality of hen's eggs during storage as indicated by the yolk. Industrial and Engineering Chemistry Research. 22: 909-910.
  44. Stewart, A.V. (1996). Plantain (Plantago lanceolata) A potential pasture species. Proceedings of the New Zealand Grassland Association, 58, 77-86.
  45. Temür, C., Uslu, S. (2019). Effects of plantain (Plantago lanceolata) containing diets of quails on growth performance, carcass characteristic, some blood parameters and mast cell numbers. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(1), 114-120. https://doi.org/10.29133/yyutbd.495271
  46. Visavadiya, N. P., B. Soni., N. Dalwadi, (2009). Evaluation of antioxidant and anti-atherogenic properties of Glycyrrhiza glabra root using in vitro models. International Journalo Food Sciences and Nutrition, 60 (Suppl.2): 135-149. https://doi.org/10.1080/09637480902877998
  47. Wang, L., R. Yang, B. Yuan, Y. Liu., C. Liu, (2015). The antiviral and antimicrobial activities of licorice, a widely-used Chinese herb. Acta Ppharmaceutica Sinica B, 5 (4): 310-315. https://doi.org/10.1016/j.apsb.2015.05.005
  48. Wilhelm, L. A., and Victor Heiman, (1936). The constancy of the albumen index of eggs from in dividual hens. U. S. Egg and Poultry Magazine. 42:750.
  49. Yang, L., L. L. Li, T. T. Liu, Y. G. Zu, F. J. Yang, C. J. Zhao, L. Zhang, X. Q. Chen., Z. H. Zhang, (2013). Development of sample preparation method for isoliquiritigenin, liquiritin, and glycyrrhizic acid analysis in licorice by ionic liquids-ultrasound based extraction and high-performance liquid chromatography detection. Food Chemistry, 138 (1): 173-179. https://doi.org/10.1016/j.foodchem.2012.10.059
  50. Zeweil, H. S., Dosoky, W. M., Zahran, S. M., Ahmed, M. H., Mohamed, A. A. (2019). Effect of licorice as an alternative to antibiotics on productive, egg quality and yolk and blood lipid profile of laying japanese quail. Journal of the Advances in Agricultural Researches, 24(2), 164-177. DOI: 10.21608/jalexu.2019.163066
  51. Zhai, Q. H., Dong, X. F., Tong, J. M., Guo, Y. M., Bao, Y. E. (2013). Long-term effects of choline on productive performance and egg quality of brown-egg laying hens. Poultry Science, 92(7), 1824-1829. DOI: 10.3382/ps.2012-02854
  52. Awadein, N. B., Eid, Y. Z., Abd El-Ghany, F. A. (2010). Effect of dietary supplementation with phytoestrogens sources before sexual maturity on productive performance of mandarah hens. Poultry Science Journal, 30(3), 829-846.