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Journals / Selcuk Dental Journal / 2026 / Volume: 12 Issue: 3

The Color Change During the Formation and Remineralization Process of Initial Carious Lesion

Başlangıç Çürük Lezyonlarının Oluşum ve Remineralizasyon Aşamalarındaki Renk Değişimi

Eylül Özgel(EGE ÜNİVERSİTESİ, DİŞ HEKİMLİĞİ FAKÜLTESİ)0000-0003-3990-3920
Çiğdem Atalayın Özkaya(EGE UNIVERSITY, FACULTY OF DENTISTRY)0000-0003-4144-4233
Ozlem Sogut(EGE UNIVERSITY, FACULTY OF PHARMACY, PHARMACY PR.)0000-0002-4661-5401
Güliz Armagan(EGE UNIVERSITY, FACULTY OF PHARMACY, PHARMACY PR.)0000-0001-6466-2263
Hüseyin Tezel(EGE UNIVERSITY, FACULTY OF DENTISTRY, DENTISTRY PR.)0000-0003-2012-0301
Pages
—
Publication Date
2025-12-29
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Keywords: casein phosphopeptide-amorphous calcium phosphate, color, floride, hydroxyapatite, initial caries, remineralization

📌 3 Key Takeaways

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Objective & Hypothesis:Abstract Objective: To evaluate the color changes at initial, after demineralization and remineralization stages with different remineralization agents.
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Key Findings & Data:Materials and Methods: The bovine teeth (n=6) crowns were divided into four equal parts and placed on silicone-indexes.
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Conclusion & Impact:Keywords: casein phosphopeptide-amorphous calcium phosphate, color, floride, hydroxyapatite, initial caries, remineralization
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:casein phosphopeptide-amorphous calcium phosphate · color · floride

Abstract

Abstract Objective: To evaluate the color changes at initial, after demineralization and remineralization stages with different remineralization agents. Materials and Methods: The bovine teeth (n=6) crowns were divided into four equal parts and placed on silicone-indexes. The samples were exposed to demineralization-solution for four days to create artificial initial caries lesions. The samples were assigned as one piece from each tooth in each group. Fluoride (Colgate Sensation White-Colgate Palmolive), hydroxyapatite (HAP) (Kinder Karex-Dr. Wolff) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) (Tooth Mousse-GC) were applied for 3 minutes twice a day for one week for remineralization. The samples were stored in artificial saliva in the control-group. The color was evaluated at initial, demineralization and remineralization stages via a spectrophotometer (Vita Easyshade Advance 5.0-Vita Zahnfabrik). ΔE1 (initial-demineralization), ΔE2 (demineralization-remineralization) and ΔE3 (initial-remineralization) were calculated with CIEDE2000 formula. Results: All ΔE values were detectable (ΔE>0.8). There was no statistically significant difference between the groups for ΔE1. There was significant difference for the HAP and CPP- ACP group compared to the control for ΔE2 (p<0.05). There was no significant difference in the fluoride group compared to the control. The significant difference was only observed in the CPP-ACP group compared to the control for ΔE3 (p<0.05). Conclusion: There is a perceptible color change during the formation and remineralization stages of the initial caries lesions. The significant color change was detected only for HAP and CPP-ACP application. Further studies with longer agent exposure and detailed measurements are required to explain the color change process in initial caries lesions. Keywords: casein phosphopeptide-amorphous calcium phosphate, color, floride, hydroxyapatite, initial caries, remineralization

Özet

Amaç: Farklı remineralizasyon ajanları uygulanan dişlerde, başlangıç, demineralizasyon ve remineralizasyon sonrası renk değişimlerini değerlendirmektir. Gereç ve Yöntemler: Sığır diş (n=6) kronları dört eşit parçaya bölünerek silikon indekslere yerleştirilmiştir. Örnekler dört gün boyunca demineralizasyon solüsyonuna tabi tutularak yapay başlangıç çürük lezyonları oluşturulmuştur. Her grupta her dişten bir parça olacak şekilde örnekler gruplandırılmıştır. Deney grubundaki örneklere flor (Colgate Sensation White-Colgate Palmolive), hidroksiapatit (HAP) (Kinder Karex-Dr. Wolff) ve kazein fosfopeptit-amorf kalsiyum fosfat (CPP-ACP) (Tooth Mousse-GC) içerikli ajanlar bir hafta süreyle, günde iki kez, 3 dk uygulanarak remineralizasyon sağlanmıştır. Kontrol grubundaki örnekler ise sadece yapay tükürük solüsyonunda bekletilmiştir. Başlangıç, demineralizasyon ve remineralizasyon sonrası spektrofotometre (Vita Easyshade Advance 5.0-Vita Zahnfabrik) ile renk ölçümleri yapılmıştır. CIEDE2000 formülü ile ΔE1 (başlangıç-demimeralizasyon) ΔE2 (demineralizasyon-remineralizasyon) ve ΔE3 (başlangıç-remineralizasyon) renk değişimi hesaplanmıştır. Bulgular: Tüm ΔE değerlerinin algılanabilir (ΔE>0.8) düzeyde olduğu saptanmıştır. ΔE1 değerleri için gruplar arası istatiksel olarak anlamlı bir fark saptanmamıştır. ΔE2 değerlerinde; HAP ve CPP-ACP grubunda kontrol grubuna göre anlamlı bir fark saptanmıştır (p<0.05). Flor grubunda kontrol grubuna kıyasla anlamlı bir fark belirlenmemiştir. ΔE3 değerlerinde ise; sadece CPP-ACP grubunda kontrol grubuna kıyasla anlamlı bir fark gözlenmiştir (p<0.05). Sonuç: Başlangıç çürük lezyonlarının oluşum ve remineralizasyon aşamalarında diş renginde algılanabilir düzeyde bir değişim oluştuğu izlenmiştir. Demineralizasyon sonrası, ajan uygulaması ile kontrol grubuna kıyasla anlamlı renk değişimi sadece HAP ve CPP-ACP gruplarında saptanmıştır. Başlangıç çürük lezyonlarında renk değişimi sürecini açıklamak için daha uzun ajan uygulama süresi ve detaylı ölçümlerle ileri çalışmalar gereklidir. Anahtar kelimeler: başlangıç mine çürüğü, flor, hidroksiapatit, kazein fosfopeptit-amorf kalsiyum fosfat, remineralizasyon, renk değişimi

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Cite This Article

Özgel, E., Özkaya, Ç. A., Sogut, O., Armagan, G., Tezel, H., *, E. Ö. (2025). The Color Change During the Formation and Remineralization Process of Initial Carious Lesion. Selcuk Dental Journal, Volume: 12 Issue: 3. https://doi.org/10.15311/selcukdentj.1630204

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