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Journals / Selcuk Dental Journal / 2025 / Volume: 12 Issue: 3

Evaluation of the MTHFR rs1801133 Gene Polymorphism and Its Association with Dental Caries

MTHFR rs1801133 Gen Polimorfizminin Diş Çürüğü Üzerindeki Etkisinin Değerlendirilmesi

Melike Usta(MARMARA ÜNİVERSİTESİ)0009-0000-7150-0860
Seda Selvi(YEDİTEPE ÜNİVERSİTESİ)0000-0002-5958-8828
Özlem Özge Yılmaz(Marmara Üniversitesi)0000-0002-4085-6159
Tolga Polat(MARMARA ÜNİVERSİTESİ)0000-0002-2064-6613
Beste Tacal Aslan(MARMARA ÜNİVERSİTESİ)0000-0001-5271-7917
Pınar Yılmaz Atalı(MARMARA ÜNİVERSİTESİ)0000-0003-3121-360X
Korkut Ulucan(MARMARA ÜNİVERSİTESİ)0000-0002-1304-9386
Pages
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Publication Date
2025-12-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This proportional difference in TT genotype associated with lower folate levels may indicate that the MTHFR rs1801133 gene polymorphism may be associated with caries risk.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: It has been shown that pathogenic plaque, diet, saliva, immune system, education level, oral hygiene habits as well as genetic factors may be effective in the formation of caries.
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Key Findings & Data:Polymorphisms in this gene significantly impact in folic acid and vitamin B12 metabolism.
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Conclusion & Impact:This proportional difference in TT genotype associated with lower folate levels may indicate that the MTHFR rs1801133 gene polymorphism may be associated with caries risk.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Dental caries · Folate metabolism · Polymorphism

Abstract

Background: It has been shown that pathogenic plaque, diet, saliva, immune system, education level, oral hygiene habits as well as genetic factors may be effective in the formation of caries. Methylenetetrahydrofolate reductase (MTHFR) encodes enzymes involved in homocysteine metabolism. Polymorphisms in this gene significantly impact in folic acid and vitamin B12 metabolism. In our study, we investigated the genetic impact on dental caries by examining the association between the MTHFR rs1801133 polimorphism and DMFT (Decayed/Missing/Filled Teeth) in caries risk groups. Methods: 2 groups were formed consisting of 56 individuals: no caries (NC- control group, DMFT=0, n=28) and individuals with caries lesions (CL-study group, DMFT≥14, n=28) homogenized in terms of periodontal status. Genotyping was conducted using Real-Time PCR and a TaqMan kit with genomic DNA from participants. Genotype and allele distributions were compared using the χ² test, with statistical significance set at p<0.05. Results: In the CL-group; 15 had CC (53.6%), 8 had CT (28.6%), and 5 had TT (17.8%) genotype. In the NC-group;15 had CC (53.6%), 11 had CT (39.3%), and 2 had TT (7.1%) genotype. There was no significant difference in genotype and allelic distribution between CL and NC groups (p=0.4149 for genotype, p=0.5341 for allele). Conclusion: Analysis of genotype distribution revealed that the TT genotype was present in 17.8% of the CL-group and 7.1% of the NC-group. This proportional difference in TT genotype associated with lower folate levels may indicate that the MTHFR rs1801133 gene polymorphism may be associated with caries risk.

Özet

Amaç: Çürük oluşumunda patojen plak, diyet, tükürük, immün sistem, eğitim düzeyi, oral hijyen alışkanlıklarının yanı sıra genetik faktörlerin de etkili olabileceği gösterilmiştir. Metilen tetrahidrofolat redüktaz (MTHFR), homosistein metabolizmasında yer alan enzimleri kodlar. Bu gendeki polimorfizmler folik asit ve B12 vitamini metabolizmasını önemli ölçüde etkiler. Bu çalışmanın amacı, MTHFR rs1801133 polimorfizmi ve DMFT (Decayed/Missing/Filled Teeth) indeksleri arasındaki ilişki incelenerek diş çürükleri üzerindeki genetik etkinin araştırılmasıdır. Gereç ve Yöntemler: Periodontal durum açısından homojenize edilmiş çürük lezyonu bulunmayan (NC-kontrol grubu, DMFT=0, n=28) ve çürük lezyonu bulunan (CL-deney grubu, DMFT≥14, n=28) 56 bireyden oluşan 2 grup oluşturuldu. Genotip belirlemesi, katılımcılarından alınan genomik DNA ile Real-Time PCR ve TaqMan kiti kullanılarak gerçekleştirildi. NC ve CL grupları arasındaki genotip ve alel dağılımları, istatistiksel anlamlılık p<0,05 olarak belirlenen χ² testi kullanılarak karşılaştırıldı. Bulgular: CL grubunda olan 28 bireyin 15'i CC (%53,6), 8'i CT (%28,6) ve 5'i TT (%17,8) genotipe sahip olmakla birlikte; NC grubunda olan 28 bireyin 15'i CC (%53,6), 11'i CT (%39,3) ve 2'si TT (%7,1) genotipe sahip olarak bulunmuştur. CL ve NC grupları arasında genotip ve alel frekansları açısından anlamlı bir fark bulunmamıştır (genotip için p = 0,4149; alel için p = 0,5341). Sonuç: Genotip dağılımı analizi sonuçlarına göre, TT genotipinin yüksek risk grubunda %17,8 ve kontrol grubunda %7,1 olarak bulunmuştur. Daha düşük folat seviyeleriyle ilişkili TT genotipindeki bu orantılı fark, MTHFR rs1801133 gen polimorfizminin çürük riskiyle ilişkili olabileceği olarak yorumlanabilir.

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Cite This Article

Usta, M., Selvi, S., Yılmaz, Ö. Ö., Polat, T., Aslan, B. T., Atalı, P. Y., Ulucan, K. (2025). Evaluation of the MTHFR rs1801133 Gene Polymorphism and Its Association with Dental Caries. Selcuk Dental Journal, Volume: 12 Issue: 3. https://doi.org/10.15311/selcukdentj.1592757

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