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Journals / Osmangazi Tıp Dergisi / 2026 / Cilt: 48 Sayı: 4

Time-Restricted Nutritional Intervention Suppresses Inflammasome Activation in the Colon Through Transcriptional Regulation

Zaman Kısıtlamalı Beslenme Müdahalesi Kolonda İnflammasom Aktivasyonunu Transkripsiyonel Düzenleme Yoluyla Baskılamakta

Neslihan Külahlıoğlu(ANKARA UNIVERSITY)0000-0002-2579-1932
Hikmet Taner Teker(ANKARA MEDİPOL UNIVERSITY)0000-0002-6621-3071
Sevcan Kabayer(MUS ALPARSLAN UNIVERSITY)0009-0006-8826-6787
Vesila Yıldırım(MUS ALPARSLAN UNIVERSITY)0009-0007-7900-8791
Taha Ceylani(MUS ALPARSLAN UNIVERSITY)0000-0002-3041-6010
Pages
698–705
Publication Date
2026-06-09
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings indicate that time-restricted feeding may suppress colonic inflammatory responses by modulating the Nlrp3 inflammasome axis at both upstream (sensor/adaptor) and downstream (effector/cytokine) levels.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to evaluate the effects of a time-restricted feeding model on inflammasome-related gene expression associated with colonic inflammation.
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Key Findings & Data:Twelve-month-old male Wistar rats were assigned either to a control group (ad libitum feeding) or to an intermittent fasting protocol consisting of 18 hours of daily fasting followed by 6 hours of feeding for 35 days (n = 7 per group).
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Conclusion & Impact:These findings indicate that time-restricted feeding may suppress colonic inflammatory responses by modulating the Nlrp3 inflammasome axis at both upstream (sensor/adaptor) and downstream (effector/cytokine) levels.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Aralıklı oruç · NLRP3 inflamasyon · kolonik inflamasyon

Abstract

This study aimed to evaluate the effects of a time-restricted feeding model on inflammasome-related gene expression associated with colonic inflammation. Twelve-month-old male Wistar rats were assigned either to a control group (ad libitum feeding) or to an intermittent fasting protocol consisting of 18 hours of daily fasting followed by 6 hours of feeding for 35 days (n = 7 per group). At the end of the experimental period, total RNA was isolated from colon tissues, and following cDNA synthesis, the expression levels of core inflammasome components (Nlrp3, Asc, and Caspase-1) and pro-inflammatory cytokine genes (Il-1β and Il-18) were analyzed using quantitative real-time PCR. Gene expression levels were statistically evaluated based on normalized ΔCt values. The intermittent fasting group exhibited significant transcriptional downregulation of all target genes compared to controls (p < 0.05). These findings indicate that time-restricted feeding may suppress colonic inflammatory responses by modulating the Nlrp3 inflammasome axis at both upstream (sensor/adaptor) and downstream (effector/cytokine) levels. Collectively, the results suggest that intermittent fasting may represent a complementary and preventive strategy in inflammation-based intestinal pathologies. Nevertheless, the molecular findings reported here require further validation through functional and clinical investigations.

Özet

Bu çalışma, zaman kısıtlamalı beslenme modelinin kolonik inflamasyonla ilişkili inflammasom gen ekspresyonu üzerindeki etkilerini değerlendirmeyi amaçlamıştır. On iki aylık erkek Wistar sıçanlar 35 gün süreyle kontrol (ad libitum beslenme) veya günlük 18 saat açlık–6 saat beslenme protokolünü içeren aralıklı açlık uygulamasına tabi tutulmuştur (n=7/grup). Deney sonunda kolon dokularından toplam RNA izole edilmiş ve cDNA sentezi sonrası inflammasom kompleksinin temel bileşenleri olan Nlrp3, Asc, Caspase-1 ile proinflamatuar sitokin genleri Il-1β ve Il-18’in ekspresyon düzeyleri kantitatif gerçek zamanlı PCR yöntemiyle analiz edilmiştir. Gen ekspresyon düzeyleri normalize edilmiş ΔCt değerleri üzerinden istatistiksel olarak değerlendirilmiştir. Aralıklı açlık uygulanan grupta tüm hedef genlerde kontrol grubuna kıyasla anlamlı düzeyde transkripsiyonel baskılanma saptanmıştır (p<0.05). Bulgular, zaman kısıtlamalı beslenmenin Nlrp3 inflammasom eksenini üst (sensör/adaptör) ve alt (efektör/sitokin) düzeylerde modüle ederek kolonik inflamatuar yanıtı baskılayabileceğini göstermektedir. Sonuç olarak, aralıklı açlık uygulamasının inflamasyon temelli bağırsak patolojilerinde tamamlayıcı ve koruyucu bir strateji olarak değerlendirilebileceği düşünülmektedir. Ancak bu moleküler düzeydeki bulguların fonksiyonel ve klinik çalışmalarla desteklenmesi gerekmektedir.

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Cite This Article

Külahlıoğlu, N., Teker, H. T., Kabayer, S., Yıldırım, V., Ceylani, T. (2026). Time-Restricted Nutritional Intervention Suppresses Inflammasome Activation in the Colon Through Transcriptional Regulation. Osmangazi Tıp Dergisi, Cilt: 48 Sayı: 4, 698–705. https://doi.org/10.20515/otd.1727767

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