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Journals / Online Türk Sağlık Bilimleri Dergisi / 2026 / Cilt: 10 Sayı: 1

Strong association between COVID-19 and Single Nucleotide Polymorphism in Nuclear Factor Kappa B genes

COVID-19 ile Nükleer Faktör Kappa B Genlerindeki Tek Nükleotid Polimorfizmleri Arasındaki Güçlü Bir İlişki

Nil Özbilüm(SİVAS CUMHURİYET ÜNİVERSİTESİ, FEN FAKÜLTESİ)0000-0002-2889-3600
Burcu Bayyurt(sivas cumhuriyet unversity faculty of medicine department of medical genetic)0000-0002-5618-457X
Sevgi Baltacı(SIVAS CUMHURIYET UNIVERSITY)0000-0002-2466-777X
Mehmet Bakır(cumhuriyet university faculty of medicine department of clinical microbiology and infectious disease)0000-0003-3702-1932
Serdal Arslan(mersin university faculty of medicine department of medical biology)0000-0002-3921-8061
Pages
15–21
Publication Date
2025-03-17
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: A significant association was found between COVID-19 disease and NF-κB genes, but further studies, such as investigating promoter activity or gene expression levels, are needed.

📌 3 Key Takeaways

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Objective & Hypothesis:Objectives: SARS-CoV-2 is an enveloped, positive-sense, single-stranded RNA virus belonging to the Betacoronavirus genus.
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Key Findings & Data:This study aimed to investigate the relationship between COVID-19 severity and NF-κB1 -94 ins/del (rs28362491), NF-κB1A 3’UTR A/G (rs696), -826 C/T (rs2233406) polymorphisms.
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Conclusion & Impact:Conclusion: A significant association was found between COVID-19 disease and NF-κB genes, but further studies, such as investigating promoter activity or gene expression levels, are needed.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:COVID-19 · NF-κB1-94 Ins/Del ATTG · NF-κB1A 3

Abstract

Objectives: SARS-CoV-2 is an enveloped, positive-sense, single-stranded RNA virus belonging to the Betacoronavirus genus. This study aimed to investigate the relationship between COVID-19 severity and NF-κB1 -94 ins/del (rs28362491), NF-κB1A 3’UTR A/G (rs696), -826 C/T (rs2233406) polymorphisms. Materials and Methods: We investigated the frequencies of these gene polymorphisms in 150 patients with COVID-19 and 171 healthy controls. Total DNA was isolated from the blood samples, and then the PCR-RFLP study was used for genotyping. All statistical analyses were calculated using the chi-square method using SPSS. Results: A statistically significant differences were determined in the D allele frequency, WD and DD genotype frequencies for the rs28362491 polymorphism. For rs696 polymorphism, there was a statistically significant difference in the frequency of the G allele of patients with COVID-19. Additionally, for this polymorphism, CT and TT genotype frequencies were shown to be statistically significant. It was also found that the T allele, CT, and TT genotype frequencies for the rs2233406 have a statistically significant difference. Conclusion: A significant association was found between COVID-19 disease and NF-κB genes, but further studies, such as investigating promoter activity or gene expression levels, are needed.

Özet

Amaç: SARS-CoV-2, Betacoronavirus cinsine ait zarflı, pozitif polariteli, tek sarmallı bir RNA virüsüdür. Bu çalışmada COVID-19 hastalığının şiddeti ve NF-κB1 -94 ins/del (rs28362491), NF-κB1A 3’UTR A/G (rs696), NFk-B1A -826 C/T (rs2233406) polimorfizmleri arasındaki ilişkinin araştırılması amaçlanmıştır. Gereç ve Yöntem: COVID-19'lu 150 hastada ve kontrol olarak 171 sağlıklı bireyde bu genlerin polimorfizm sıklıklarını araştırdık. Kan örneklerinden total DNA izole edildi ve ardından genotipleme için PCR-RFLP çalışması kullanıldı. Tüm istatistiki analizler SPSS kullanılarak ki-kare yöntemi ile hesaplanmıştır. Bulgular: Rs28362491 polimorfizmi için D alel frekansı, WD ve DD genotip frekanslarında istatistiksel olarak anlamlı farklılıklar tespit edilmiştir. Rs696 polimorfizmi için, COVID-19'lu hastaların G aleli sıklığında istatistiksel olarak anlamlı bir fark vardı. Ayrıca, bu polimorfizm için CT ve TT genotip frekanslarının istatistiksel olarak anlamlı olduğu gösterilmiştir. Ayrıca, rs2233406 için T aleli, CT ve TT genotip frekanslarının istatistiksel olarak anlamlı bir farka sahip olduğu bulunmuştur. Sonuç: COVID-19 hastalığı ile NF-κB genleri arasında anlamlı bir ilişki bulunmuştur, ancak promotör aktivitesinin veya gen ekspresyon seviyelerinin araştırılması gibi daha ileri çalışmalara ihtiyaç vardır.

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Cite This Article

Özbilüm, N., Bayyurt, B., Baltacı, S., Bakır, M., Arslan, S. (2025). Strong association between COVID-19 and Single Nucleotide Polymorphism in Nuclear Factor Kappa B genes. Online Türk Sağlık Bilimleri Dergisi, Cilt: 10 Sayı: 1, 15–21. https://doi.org/10.26453/otjhs.1505688

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