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Journals / Middle East Journal of Science / 2025 / Volume: 11 Issue: 2

EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES

Erhan Mustafa Akarsu0000-0002-6956-0909
Halit Kızılet(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0002-7289-4635
Pinar Aksu Kılıçle0000-0002-3567-5775
Ali Aslan0000-0002-5122-6646
Pages
—
Publication Date
2025-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, MN percentages were significantly higher in EMS and MMC applied groups compared to negative control (p<0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Environmental pollutants and genotoxic agents pose serious risks to human health and the ecosystem through DNA damage.
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Key Findings & Data:In the experimental groups determined by preliminary experiments, negative control (distilled water), positive controls (3 μg/ml EMS and MMC) and combinations containing different concentrations (30, 60, 120 μg/ml) of XSG extract were used.
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Conclusion & Impact:According to the results, MN percentages were significantly higher in EMS and MMC applied groups compared to negative control (p<0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Genotoxicity · Xanthoparmelia somloensis · Micronucleus test

Abstract

Environmental pollutants and genotoxic agents pose serious risks to human health and the ecosystem through DNA damage. This study aimed to evaluate the protective potential of the aqueous extract of Xanthoparmelia somloensis (XSG) lichen against ethyl methanesulfonate (EMS) and mitomycin-C (MMC) induced genotoxicity by in vitro micronucleus (MN) test. The genotoxic effects of EMS and MMC and the antigenotoxic activity of XSG extract were analyzed in peripheral blood lymphocytes taken from healthy donors. In the experimental groups determined by preliminary experiments, negative control (distilled water), positive controls (3 μg/ml EMS and MMC) and combinations containing different concentrations (30, 60, 120 μg/ml) of XSG extract were used. According to the results, MN percentages were significantly higher in EMS and MMC applied groups compared to negative control (p<0.05). XSG water extract did not show genotoxic effect when applied alone, but reduced MN frequency dose-dependently in combination with EMS and MMC. Especially 120 μg/ml XSG extract exhibited the highest antigenotoxic effect by reducing EMS-induced MN by 65.7% and MMC-induced MN by 71.7%. The protective mechanism of XSG may be related to the scavenging of free radicals and modulation of DNA repair pathways through antioxidant components (e.g. usnic acid). These findings reveal the potential of XSG as a broad-spectrum antigenotoxic agent and contribute to the development of natural protective strategies.

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Cite This Article

Akarsu, E. M., Kızılet, H., Kılıçle, P. A., Aslan, A. (2025). EVALUATION OF THE ANTIGENOTOXIC POTENTIAL OF Xanthoparmelia somloensis LICHEN EXTRACT IN HUMAN LYMPHOCYTES. Middle East Journal of Science, Volume: 11 Issue: 2. https://doi.org/10.51477/mejs.1661476

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