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Journals / Middle East Journal of Science / 2025 / Volume: 11 Issue: 2

THE EFFECT OF ZINC AND BMP2 ON CONNEXIN 43 PRODUCTION IN BONE MARROW MESENCHYMAL STEM CELLS (BM-MSCS) INDUCED BY DMBA

Kemal Yuce(SELCUK UNIVERSITY)0000-0001-8915-4900
Pages
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Publication Date
2025-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When using BM-MSCs in cancer studies, it should be taken into consideration that the cancerization process may affect the production of connexin 43 and the ability of the cells to adhere.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to examine how zinc and BMP2 affected the amount of connexin 43 produced by BM-MSCs stimulated with DMBA.
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Key Findings & Data:BM-MSCs were identified after being generated in a cell culture.
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Conclusion & Impact:When using BM-MSCs in cancer studies, it should be taken into consideration that the cancerization process may affect the production of connexin 43 and the ability of the cells to adhere.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:BMP2 · Bone marrow mesenchymal stem cells · Connexin 43

Abstract

The aim of this study was to examine how zinc and BMP2 affected the amount of connexin 43 produced by BM-MSCs stimulated with DMBA. BM-MSCs were identified after being generated in a cell culture. BM-MSCs were exposed to combinations of 24 nM DMBA, 1 µM zinc, and 10 ng/mL BMP2. Using immunofluorescence methods, the effects of zinc, BMP2, and DMBA on the formation of connexin 43 in BM-MSCs were assessed. In the present study, BM-MSCs were produced and identified. As a result of immunofluorescence, connexin 43 expression was somewhat downregulated in the BMP2, zinc, and DMBA single applications; this downregulation was more pronounced in the double and triple applications. Zinc concentrations in the medium where BM-MSCs are produced may affect the results of preclinical and clinical studies based on transplantation through the adhesion ability of the cells, depending on the toxic effect. When using BM-MSCs in cancer studies, it should be taken into consideration that the cancerization process may affect the production of connexin 43 and the ability of the cells to adhere.

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Cite This Article

Yuce, K. (2025). THE EFFECT OF ZINC AND BMP2 ON CONNEXIN 43 PRODUCTION IN BONE MARROW MESENCHYMAL STEM CELLS (BM-MSCS) INDUCED BY DMBA. Middle East Journal of Science, Volume: 11 Issue: 2. https://doi.org/10.51477/mejs.1547916

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