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Journals / Marine Science and Technology Bulletin / 2020 / Volume: 9 Issue: 2

Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity

Muammer Kırıcı(Bingöl University, Vocational School of Technical Sciences, Department of Veterinary Health, 12000, Bingöl, Turkey)0000-0003-1888-4388
Muhammed Atamanalp(Atatürk University, Faculty of Fisheries, Department of Aquaculture, 25240, Erzurum, Turkey)0000-0002-2038-3921
Mahinur Kırıcı(Bingöl University, Faculty of Arts and Science, Department of Chemistry, 12000, Bingol, Turkey)0000-0003-4642-7387
Şükrü Beydemir(Anadolu University, Faculty of Pharmacy, Department of Biochemistry, 26470, Eskişehir, Turkey)0000-0003-3667-6902
Pages
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Publication Date
2020-12-31
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag + .

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, the in vitro effects of some metal ions (Ag + , Cd 2+ , Cu 2+ , Fe 2+ , Ni 2 + and Pb 2+ ) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated.
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Key Findings & Data:For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively.
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Conclusion & Impact:Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag + .
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Glucose 6-phosphate dehydrogenase · Capoeta umbla · Enzyme purification

Abstract

In this study, the in vitro effects of some metal ions (Ag + , Cd 2+ , Cu 2+ , Fe 2+ , Ni 2 + and Pb 2+ ) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag + , Cd 2+ , Cu 2+ , Ni 2+ and Pb 2+ inhibited fish liver G6PD; Ag + , Cd 2+ , Fe 2+ , Ni 2 + and Pb 2 + inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag + . Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.

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Cite This Article

Kırıcı, M., Atamanalp, M., Kırıcı, M., Beydemir, Ş. (2020). Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity. Marine Science and Technology Bulletin, Volume: 9 Issue: 2. https://doi.org/10.33714/masteb.709377

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