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Journals / Marine Science and Technology Bulletin / 2021 / Volume: 10 Issue: 4

Phycocyanin Extraction From Frozen and Freeze-Dried Biomass of Pseudanabaena sp. by Using Mild Cell Disruption Methods

Berke Kısaoğlan(Ege University, Graduate School of Natural and Applied Sciences, Department of Biotechnology, İzmir 35100, Turkey)0000-0003-3886-6128
Zeliha Demirel(Ege University, Faculty of Engineering, Department of Bioengineering, İzmir 35100, Turkey)0000-0003-3675-7315
Meltem Conk Dalay(Ege University, Faculty of Engineering, Department of Bioengineering, İzmir 35100, Turkey)0000-0002-1718-7292
Pages
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Publication Date
2021-12-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Phycocyanin content of freeze-dried biomass was analysed after six months of storage and dramatic decrement was observed in the phycocyanin content of the cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Phycocyanin is a precious, natural, blue coloured pigment-protein complex that has commercial value and wide application in cosmetics, food, and pharmaceutical industries.
📊
Key Findings & Data:In the present study, we performed various cell disruption methods (ultrasonication, homogenization, freeze/thaw and CaCl2 extraction) for phycocyanin extraction from different forms of biomass of a thermophilic Pseudanabaena sp. that has a high potential to produce high-quality phycocyanin.
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Conclusion & Impact:Phycocyanin content of freeze-dried biomass was analysed after six months of storage and dramatic decrement was observed in the phycocyanin content of the cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cyanobacteria · Extraction · Phycobiliprotein

Abstract

Phycocyanin is a precious, natural, blue coloured pigment-protein complex that has commercial value and wide application in cosmetics, food, and pharmaceutical industries. In the present study, we performed various cell disruption methods (ultrasonication, homogenization, freeze/thaw and CaCl2 extraction) for phycocyanin extraction from different forms of biomass of a thermophilic Pseudanabaena sp. that has a high potential to produce high-quality phycocyanin. Using potassium phosphate buffer and ultrasonic bath method, we achieved the highest phycocyanin yield (345 mgPC.g -biomass ) from freeze-dried biomass and we obtained increased yield as the duration of application increases. Phycocyanin yields were calculated as 345 mgPC.g -biomass , 255 mgPC.g -biomass and 220 mgPC.g -biomass for 5, 10 and 15 min, respectively. In this study, cell disruption methods have determined significantly more effective on freeze-dried biomass rather than frozen biomass. Phycocyanin content of freeze-dried biomass was analysed after six months of storage and dramatic decrement was observed in the phycocyanin content of the cells.

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Cite This Article

Kısaoğlan, B., Demirel, Z., Dalay, M. C. (2021). Phycocyanin Extraction From Frozen and Freeze-Dried Biomass of Pseudanabaena sp. by Using Mild Cell Disruption Methods. Marine Science and Technology Bulletin, Volume: 10 Issue: 4. https://doi.org/10.33714/masteb.951265

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