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Journals / Sakarya Tıp Dergisi / 2026 / Volume: 10 Issue: 1

Effect of 2-Aminoethoxydiphenyl Borate on Acute Cerebral Ischemia-Reperfusion Injury; An Experimental Study

2-Aminoetoksidifenil Borat’ ın Akut Serebral İskemi-Reperfüzyon Hasarı Üzerindeki Etkisi; Deneysel Bir Çalışma

Tuba Demirci(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI)0000-0002-8814-9648
Nuray Bilge Bilge(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, NÖROLOJİ ANABİLİM DALI)0000-0002-9328-1678
Özlem Özgül Abuç(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI)0000-0001-9852-8839
Nuh Çağrı Karaavcı(ATATÜRK ÜNİVERSİTESİ, TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, NÖROŞİRURJİ ANABİLİM DALI)0000-0002-4316-3614
Pages
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Publication Date
2020-03-16
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Conclusion: As a result, it has been demonstrated that 2-APB may be effective in decreasing apoptosis and neuronal damage by reducing SOCE-mediated Ca 2+ influx into the cell in cerebral I/R damaged tissue.

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: The store-operated Ca 2+ entry (SOCE) is responsible for Ca 2+ entry into the cell and takes place mediated by the Orai1, STIM1, and STIM2 proteins. 2-Aminoethoxydiphenyl Borate (2-APB) is a chemical agent that prevents store-operated Ca 2+ entry into the cell.
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Key Findings & Data:In this study, it was investigated the possible protective effect of 2-APB on the occurred injury in the experimental cerebral I/R model in rats.
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Conclusion & Impact:Conclusion: As a result, it has been demonstrated that 2-APB may be effective in decreasing apoptosis and neuronal damage by reducing SOCE-mediated Ca 2+ influx into the cell in cerebral I/R damaged tissue.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:2-Aminoethoxydiphenyl borate · Cerebral ischemia / reperfusion · SOCE

Abstract

Objective: The store-operated Ca 2+ entry (SOCE) is responsible for Ca 2+ entry into the cell and takes place mediated by the Orai1, STIM1, and STIM2 proteins. 2-Aminoethoxydiphenyl Borate (2-APB) is a chemical agent that prevents store-operated Ca 2+ entry into the cell. In this study, it was investigated the possible protective effect of 2-APB on the occurred injury in the experimental cerebral I/R model in rats. Materials and Methods: In the study, eighteen male rats were divided into three groups (n=6). In the Sham group, only surgical procedure was applied without I/R model. Acute cerebral I/R model was created for rats in the SI/R and SI/R-2APB groups. Reperfusion was realized one-hour later. Ten-minutes before reperfusion, 2-APB was administered to the rats in the SI/R-2APB group. After three hours of reperfusion, the rats were sacrificed and their brains were removed. The changes that occurred in the brain tissues were investigated by using histochemical methods, the TUNEL, and immunohistochemical staining. Results: In the SI/R group, it was determined that the histological structure was impaired and apoptosis and expression of Orai1, STIM1, and STIM2 proteins were increased compared to the sham group. In the 2-APB administrated group, it was revealed that the tissue damage and apoptotic cells were decreased, and Orai1, STIM1, and STIM2 expression were significantly inhibited. Conclusion: As a result, it has been demonstrated that 2-APB may be effective in decreasing apoptosis and neuronal damage by reducing SOCE-mediated Ca 2+ influx into the cell in cerebral I/R damaged tissue.

Özet

Amaç: Serebral İ/R hasarında hücre ölümüne neden olan faktörlerin başında hücre içi kalsiyum konsantrasyonundaki artış gelmektedir. Depo kontrollü Ca 2+ girişi (Store operated Ca 2+ entry, SOCE ), hücre içine Ca 2+ girişinden sorumludur ve Orai1, STIM1 ve STIM2 proteinleri aracılığıyla gerçekleşir. 2 -Aminoetoksidifenil Borat (2-APB), hücreye depo kontrollü Ca 2+ girişini engelleyen kimyasal bir ajandır. Bu çalışmada deneysel olarak sıçanlarda oluşturulan serebral İ/R modelinde meydana gelen hasarda 2-APB’nin olası koruyucu etkisi araştırıldı. Gereç ve Yöntemler: Çalışmada, on sekiz erkek sıçan 3 gruba ayrıldı (n=6). Sham grubunda cerrahi prosedür uygulandı ancak İ/R modeli oluşturulmadı. Sİ/R ve Sİ/R-2APB grubundaki sıçanlara akut serebral İ/R modeli oluşturuldu. Bir saatl sonra reperfüzyon sağlandı. Sİ/R-2APB grubundaki sıçanlara reperfüzyondan 10 dk önce 2-APB uygulandı. Reperfüzyondan 3 saat sonra sıçanlara ötenazi uygulanarak beyinleri çıkarıldı. Sıçan beyin dokularında meydana gelen değişiklikler histokimyasal yöntemlerle, TUNEL metoduyla ve immünohistokimyasal olarak değerlendirildi. Bulgular: Sİ/R grubunda, sham grubuna kıyasla histolojik yapının bozulduğu, apopitozisin ve Orai1, STIM1 ve STIM2 proteinlerinin ekspresyonunun arttığı belirlendi. 2-APB uygulanan grupta ise doku hasarının ve apopitotik hücrelerin azaldığı, Orai1, STIM1 ve STIM2 ifadesinin belirgin şekilde inhibe edildiği ortaya konuldu. Sonuç: Sonuç olarak, serebral İ/R hasarlı dokuda 2-APB’nin hücreye SOCE aracılı Ca 2+ girişini azaltarak apopitozun ve nöronal hasarın azatılmasında etkili olabileceği ortaya konuldu.

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Cite This Article

Demirci, T., Bilge, N. B., Abuç, Ö. Ö., Karaavcı, N. Ç. (2020). Effect of 2-Aminoethoxydiphenyl Borate on Acute Cerebral Ischemia-Reperfusion Injury; An Experimental Study. Sakarya Tıp Dergisi, Volume: 10 Issue: 1. https://doi.org/10.31832/smj.637779

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