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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 8 Sayı: 3

In Vitro Shoot Regeneration of Limnophila aromatica (Lamk.) Merr. from Nodal and Internodal Explants

Limnophila aromatica (Lamk.) Merr.’nın Boğum ve Boğum Arası Eksplantlarından In Vitro Sürgün Rejenerasyonu

Muhammet Doğan0000-0003-3138-5903
Pages
77–84
Publication Date
2018-09-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Successful adaptation of the rooted shoots to the aquarium environment has been achieved.

📌 3 Key Takeaways

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Objective & Hypothesis:Limnophila aromatica (Lamk.) Merr. is an important medicinal and aromatic plant widely used in the traditional medicine.
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Key Findings & Data:In this study, nodal and internodal explants of L. aromatica (Lamk.) Merr. were cultured for multiple and rapid production on Murashige and Skoog (MS) medium containing combinations of 0.10 mg L-1 Gibberellic acid (GA3) and 0.05, 0.10, 0.20, 0.40, 0.80 and 1.60 mg L-1 Thidiazuron (TDZ) for eight weeks.
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Conclusion & Impact:Successful adaptation of the rooted shoots to the aquarium environment has been achieved.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Boğum eksplant · çoğaltım · in vitro

Abstract

Limnophila aromatica (Lamk.) Merr. is an important medicinal and aromatic plant widely used in the traditional medicine. In this study, nodal and internodal explants of L. aromatica (Lamk.) Merr. were cultured for multiple and rapid production on Murashige and Skoog (MS) medium containing combinations of 0.10 mg L-1 Gibberellic acid (GA3) and 0.05, 0.10, 0.20, 0.40, 0.80 and 1.60 mg L-1 Thidiazuron (TDZ) for eight weeks. In general, high shoot regeneration percentages were obtained for both types of explants. In nodal explants, the maximum number of shoots per explant (21.44) and the highest shoot length (1.68 cm) were determined in MS medium containing 0.10 mg L-1 GA3 + 0.40 mg L-1 TDZ and 0.10 mg L-1 GA3 + 0.10 mg L-1 TDZ, respectively. In internodal explants, the maximum number of shoots per explant (17.46) and the highest shoot length (1.60 cm) were obtained in MS medium supplemented with 0.10 mg L-1 GA3 + 0.20 mg L-1 TDZ and 0.10 mg L-1 GA3 + 0.05 mg L-1 TDZ, respectively. With the effect of plant growth regulators, the nodal explants gave more regenerated shoots than the internodal explants. Regenerated shoots were successfully rooted on MS medium containing 0.25 mg L-1 indole butyric acid (IBA). Successful adaptation of the rooted shoots to the aquarium environment has been achieved.

Özet

Limnophila aromatica (Lamk.) Merr. geneneksel tıpta yaygın şekilde kullanılan önemli tıbbi ve aromatik

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Cite This Article

Doğan, M. (2018). In Vitro Shoot Regeneration of Limnophila aromatica (Lamk.) Merr. from Nodal and Internodal Explants. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 3, 77–84. https://ajindex.com/articles/1272842

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