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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2018 / Cilt: 8 Sayı: 4

Effect of Ethyl Methanesulphonate (EMS) Applications on In Vitro Growth of Sunflower (Helianthus annuus L. cv. Palancı-I) under Salinity Conditions

Ayçiçeğinin (Helianthus annuus L. cv. Palancı-I) In Vitro Tuzlu Koşullarda Gelişimi Üzerine Etil Metansülfonat (EMS) Uygulamalarının Etkisi

Demet Altındal(UŞAK ÜNİVERSİTESİ, SİVASLI MESLEK YÜKSEKOKULU, BİTKİSEL VE HAYVANSAL ÜRETİM BÖLÜMÜ, TIBBİ VE AROMATİK BİTKİLER PR.)0000-0002-2198-7209
Nüket Altındal(UŞAK ÜNİVERSİTESİ, SİVASLI MESLEK YÜKSEKOKULU, BİTKİSEL VE HAYVANSAL ÜRETİM BÖLÜMÜ, TIBBİ VE AROMATİK BİTKİLER PR.)0000-0002-9567-1653
Pages
351–359
Publication Date
2018-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was concluded that EMS treatments decreased negative effects of increasing NaCl doses.

📌 3 Key Takeaways

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Objective & Hypothesis:Four ethyl methanesulphonate (EMS) doses (control, 1.0, 1.5 ve 2.0 %) were exposed to kernels of Palancı-I sunflower variety and this kernels were cultured on MS medium added different 4 NaCl doses (0.0, 1.25, 2.5 ve 5.0 g/L) in in vitro for 4 weeks.
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Key Findings & Data:Except for node number and leaf number the effects of EMS applications and salinity levels significantlly affected to all characteristics.
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Conclusion & Impact:It was concluded that EMS treatments decreased negative effects of increasing NaCl doses.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Ayçiçeği · in vitro · etil metansülfonat

Abstract

Four ethyl methanesulphonate (EMS) doses (control, 1.0, 1.5 ve 2.0 %) were exposed to kernels of Palancı-I sunflower variety and this kernels were cultured on MS medium added different 4 NaCl doses (0.0, 1.25, 2.5 ve 5.0 g/L) in in vitro for 4 weeks. In this study, plantlet height (cm), plantlet fresh weight (g), root length (cm), leaf number (number), leaf width and length (mm), node number (number), total chlorophyll content (µg/g) and proline content (Mmol/g FW) were investigated. Except for node number and leaf number the effects of EMS applications and salinity levels significantlly affected to all characteristics. Generally, according to increasing NaCl concentrations, other characters decreased except proline content. The higher EMS doses caused significantly declines in investigated characters except total chlorophyll content but weren’t affect proline content. It was concluded that EMS treatments decreased negative effects of increasing NaCl doses.

Özet

Bu çalışmada, Palancı-I ayçiçeği çeşidi tohumlarına ait çekirdek içlerine dört farklı dozda (kontrol, % 1.0, 1.5 ve 2.0) etil metansülfonat (EMS) uygulanmış ve çekirdek içleri farklı NaCl dozu (0.0, 1.25, 2.5 ve 5.0 g/L) içeren besi ortamlarında 4 hafta süreyle in vitro koşullarda kültüre alınmıştır. Elde edilen bitkiciklerin boyu (cm), yaş ağırlığı (g), kök uzunluğu (cm), yaprak sayısı (adet), yaprak eni ve boyu (mm), boğum sayısı (adet), toplam klorofil içeriği (µg/g) ve prolin içeriği (Mmol/g YA) incelenmiştir. Boğum sayısı ve yaprak sayısı hariç incelenen tüm özelliklerde artan tuz dozları ve EMS uygulamalarının etkisi istatistiki olarak önemli bulunmuştur. Genel olarak, artan NaCl konsantrasyonlarına bağlı olarak prolin içeriği hariç, diğer incelenen özelliklerde azalma gözlenmiştir. Yine uygulanan yüksek EMS dozları toplam klorofil içeriği hariç incelenen diğer özelliklerde önemli seviyede düşüşlere sebep olmuş ancak prolin içeriğini etkilememiştir. Sonuç olarak, EMS uygulamaları artan NaCl dozlarının olumsuz etkilerini azaltmıştır.

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Cite This Article

Altındal, D., Altındal, N. (2018). Effect of Ethyl Methanesulphonate (EMS) Applications on In Vitro Growth of Sunflower (Helianthus annuus L. cv. Palancı-I) under Salinity Conditions. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 8 Sayı: 4, 351–359. https://doi.org/10.21597/jist.432102

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