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Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2026 / Cilt: 9 Sayı: 3

Isolation, Macroscopic, Microscopic Identification, DNA Sequencing and Bioinformatic Assessments of Native Fungi for the Production of Native Alpha-Amylase Enzyme

Yerli Alfa-Amilaz Enzimi Üretimi İçin Yerli Fungus İzolasyonu, Makroskobik, Mikroskobik Tanımlaması, DNA Dizi Analizi ve Biyoinformatik Değerlendirmeler

Arzu Ünal(IĞDIR ÜNİVERSİTESİ, IĞDIR ZİRAAT FAKÜLTESİ)0000-0003-4427-3169
Semra Malkoç(ESKİŞEHİR TEKNİK ÜNİVERSİTESİ)0000-0002-8092-411X
İjlal Ocak(AFYON KOCATEPE ÜNİVERSİTESİ)0000-0001-6976-5747
Safiye Elif Korcan(UŞAK ÜNİVERSİTESİ)0000-0001-7875-5516
Pages
1674–1682
Publication Date
2019-09-01
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of molecular characterization study, it was observed that the classical identification and the molecular identification supported each other.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, isolation of thermophilic and mesophilic native fungi producing alpha-amylase enzyme which is commercially important in bread making, macroscopic, microscopic and molecular identification were performed.
📊
Key Findings & Data:Microorganism Culture Collection Centers and Microbial GenBanks to create genetic material and local isolates to produce patents of the native enzyme was aimed. 23 thermotolerant fungi were isolated from the thermal areas of Afyon, Eskişehir, Uşak and Ankara.
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Conclusion & Impact:As a result of molecular characterization study, it was observed that the classical identification and the molecular identification supported each other.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Fungus · izolasyon · Internal Transcribed Spacer (ITS)

Abstract

In this study, isolation of thermophilic and mesophilic native fungi producing alpha-amylase enzyme which is commercially important in bread making, macroscopic, microscopic and molecular identification were performed. Microorganism Culture Collection Centers and Microbial GenBanks to create genetic material and local isolates to produce patents of the native enzyme was aimed. 23 thermotolerant fungi were isolated from the thermal areas of Afyon, Eskişehir, Uşak and Ankara. As a result of macroscopic and microscopic investigations of isolates, 6 isolates of Aspergillus niger, Aspergillus terreus and Trichoderma atroviride species which produce high amylase enzyme species were determined. 18S rDNA and ITS regions of the isolates were compared with Web-based BLAST analysis by PCR amplification and molecular identification. As a result of molecular characterization study, it was observed that the classical identification and the molecular identification supported each other.

Özet

Bu çalışmada ekmek yapımında ticari önemi olan alfa-amilaz enzimi üreten termofilik ve mezofilik yerli fungusların izolasyonu, makroskobik, mikroskobik ve moleküler tür teşhisleri gerçekleştirilmiştir. Mikroorganizma Kültür Koleksiyon Merkezlerine ve Mikrobiyal Gen Bankalarına genetik materyal oluşturulması ve yerli izolatlardan patente konu yerli enzim üretimi amaçlanmıştır. Afyon, Eskişehir, Uşak, Ankara termal alanlardan 23 termotolerant fungus izole edilmiştir. İzolatların makroskobik ve mikroskobik incelemeleri sonucunda yüksek amilaz enzimi üreten Aspergillus niger, Aspergillus terreus ve Trichoderma atroviride türüne ait 6 izolatın tür teşhisi yapılmıştır. İzolatların fungus spesifik primerleri kullanılarak 18S rDNA ve ITS bölgeleri PCR amplifikasyonu, moleküler tanımlaması yapılmış ve Web tabanlı BLAST analizleri ile karşılaştırılmıştır. Moleküler karakterizasyon çalışması sonucunda klasik tanımlama ile moleküler tanımlamanın birbirini desteklediği görülmüştür.

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Cite This Article

Ünal, A., Malkoç, S., Ocak, İ., Korcan, S. E. (2019). Isolation, Macroscopic, Microscopic Identification, DNA Sequencing and Bioinformatic Assessments of Native Fungi for the Production of Native Alpha-Amylase Enzyme. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 9 Sayı: 3, 1674–1682. https://doi.org/10.21597/jist.542215

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