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Journals / Gazi University Journal of Science / 2026 / Volume: 39 Issue: 1

Determination of Biological Activity and Biochemical Composition of Polytrichastrum formosum (Hedw.) G.L.Sm.

Atakan Benek(DOKUZ EYLÜL ÜNİVERSİTESİ)0000-0001-6726-5968
Kerem Canlı(DOKUZ EYLUL UNIVERSITY)0000-0001-6061-6948
Ergin Murat Altuner(KASTAMONU ÜNİVERSİTESİ)0000-0001-5351-8071
Pages
—
Publication Date
2026-01-10
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further investigation into its secondary metabolites may contribute to the development of innovative therapeutic agents.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study examines the chemical profile and bioactivity of Polytrichastrum formosum (Hedw.) G.L.
📊
Key Findings & Data:Various tests, including disk diffusion, minimum inhibitory concentration (MIC), Cupric reducing antioxidant capacity (CUPRAC), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and Folin-Ciocalteu methods, were employed to assess its antibacterial, antioxidant, and phenolic content.
💡
Conclusion & Impact:Further investigation into its secondary metabolites may contribute to the development of innovative therapeutic agents.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Polytrichastrum formosum (Hedw.) G.L.Sm. · Moss · Biological activity

Abstract

This study examines the chemical profile and bioactivity of Polytrichastrum formosum (Hedw.) G.L. Sm., a moss species, to explore its potential therapeutic applications, focusing on antibacterial, antioxidant, and anti-quorum sensing activities. The moss specimen was collected and extracted using ethanol. Various tests, including disk diffusion, minimum inhibitory concentration (MIC), Cupric reducing antioxidant capacity (CUPRAC), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and Folin-Ciocalteu methods, were employed to assess its antibacterial, antioxidant, and phenolic content. Quorum sensing inhibition was evaluated by testing pyocyanin - motility inhibition in Pseudomonas aeruginosa, and violacein inhibition in Chromobacterium violaceum. The components of the P. formosum ethanol extract were analysed using gas chromatography (GC) and mass spectrometry (MS). The ethanol extract of P. formosum exhibited notable antibacterial effects against 19 strains, including multidrug-resistant bacteria. It also showed robust antioxidant capacity, as evidenced by substantial DPPH and CUPRAC radical scavenging activities. The concentration of phenolic compounds was measured at 190.05 mg GAE/mL. Moreover, the extract moderately suppressed pyocyanin production in P. aeruginosa and slightly reduced its twitching motility, indicating potential anti-quorum sensing activity. GC-MS analysis revealed the presence of bioactive constituents, including 2,4-di-tert-butylphenol and linoleic acid, which are associated with various biological effects. This study emphasizes the notable antibacterial, antioxidant, and anti-quorum sensing activities of P. formosum extract, indicating its value as a natural candidate for addressing antibiotic resistance and oxidative stress. Further investigation into its secondary metabolites may contribute to the development of innovative therapeutic agents.

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Cite This Article

Benek, A., Canlı, K., Altuner, E. M. (2026). Determination of Biological Activity and Biochemical Composition of Polytrichastrum formosum (Hedw.) G.L.Sm.. Gazi University Journal of Science, Volume: 39 Issue: 1. https://doi.org/10.35378/gujs.1719380

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