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Journals / Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi / 2026 / Cilt: 39 Sayı: 1

Primary Culture of Cancer Cells Derived from Human Lung Tumors

İnsan Akciğer Tümörlerinden Elde Edilen Kanser Hücrelerinin Primer Kültürü

Seçil Yılmaz(Erciyes Üniversitesi, Genom ve Kök Hücre Merkezi (GENKÖK))0000-0001-9381-828X
Medine Doğan Sarıkaya(Erciyes Üniversitesi, Genom ve Kök Hücre Merkezi (GENKÖK))0000-0003-0435-6066
Esra Atar(Erciyes Üniversitesi, Genom ve Kök Hücre Merkezi (GENKÖK))0000-0002-6568-2626
Burcu Şen Bağcı(ERCİYES ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, TIBBİ MİKROBİYOLOJİ ANABİLİM DALI)0000-0002-4526-5198
Sercan Kenanoğlu(ERCİYES ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, TIBBİ GENETİK ANABİLİM DALI)0000-0003-2239-0209
Nilhan Mutlu(Erciyes Üniversitesi, Genom ve Kök Hücre Merkezi (GENKÖK))0000-0001-8308-1456
Elif Yaşar(Erciyes Üniversitesi, Genom ve Kök Hücre Merkezi (GENKÖK))0000-0001-5974-4176
Ömer Önal(ERCİYES ÜNİVERSİTESİ, TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, GENEL CERRAHİ ANABİLİM DALI)0000-0002-9971-7401
Özlem Canöz(ERCİYES ÜNİVERSİTESİ, TIP FAKÜLTESİ, CERRAHİ TIP BİLİMLERİ BÖLÜMÜ, PATOLOJİ ANABİLİM DALI)0000-0002-0200-6970
Yusuf Özkul(ERCİYES ÜNİVERSİTESİ, TIP FAKÜLTESİ, DAHİLİ TIP BİLİMLERİ BÖLÜMÜ, TIBBİ GENETİK ANABİLİM DALI)0000-0002-4212-5763
Pages
79–86
Publication Date
2023-05-02
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Primary cell culture is an in vitro model that is thought to contribute to thedevelopment of personalized treatments in future studies for the effective treatmentof lung cancer by bridging the gap between the laboratory and the clinical decisionprocess of the physician.

📌 3 Key Takeaways

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Objective & Hypothesis:Primary cell culture is an important research platform for studying thebiochemistry and molecular biology relevant to individual cancer types andunderstanding cancer cell physiology.
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Key Findings & Data:Results: In this study, cancer cells were successfully isolated andcultured from the tumor tissues of 3 patients diagnosed with NSCLC, whose tumordiameters were 2*1.5*1.2, 8*7*6, 4*3.7*3, without metastasis and aged between 43-70 years.
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Conclusion & Impact:Primary cell culture is an in vitro model that is thought to contribute to thedevelopment of personalized treatments in future studies for the effective treatmentof lung cancer by bridging the gap between the laboratory and the clinical decisionprocess of the physician.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Akciğer kanseri · Küçük Hücreli Dışı Akciğer Kanseri · Primer hücre kültürü

Abstract

Primary cell culture is an important research platform for studying thebiochemistry and molecular biology relevant to individual cancer types andunderstanding cancer cell physiology. Therefore, in this study, it was aimed toperform primary cell culture from tissues of non-small cell lung cancer (NSCLC)patients with different clinical course in our own laboratory conditions. Thus, it willthrow light on future studies on tumor biology and pathogenesis in NSCLC, the mostcommon subtype of lung cancer, which is the most malignant type of cancer.Materials and Methods: Patients diagnosed with NSCLC who required surgicalintervention as a result of pulmonary function tests and thorax computedtomography and gave written consent were included in the study. After thepathologically confirmed tumor tissue was removed by surgical resection, the cellsobtained by mechanical and enzymatic digestion with collagenase/hyaluronidasewere cultured in Dulbecco’s Modified Eagle Medium (DMEM) under sterileconditions. Results: In this study, cancer cells were successfully isolated andcultured from the tumor tissues of 3 patients diagnosed with NSCLC, whose tumordiameters were 2*1.5*1.2, 8*7*6, 4*3.7*3, without metastasis and aged between 43-70 years. Conclusions: This study presents a successful primary cell cultureprotocol that includes important steps for obtaining patient-derived tumor cells inlung cancer based on information in the literature and personal experience on thesubject. Primary cell culture is an in vitro model that is thought to contribute to thedevelopment of personalized treatments in future studies for the effective treatmentof lung cancer by bridging the gap between the laboratory and the clinical decisionprocess of the physician.

Özet

Amaç: Primer hücre kültürü bireysel kanser türleri ile ilişkili biyokimya ve moleküler biyolojiyi inceleme ve kanser hücresi fizyolojisini anlamak için kullanılan önemli bir araştırma platformudur. Bu nedenle bu çalışmada en ölümcül kanser türü olan akciğer kanserinin en sık görülen alt tipi olan küçük hücreli dışı akciğer kanserinde (KHDAK) tümör biyolojisi ve patogenezi ile ilgili ileride yapılacak olan çalışmalara ışık tutması için farklı klinik seyir gösteren KHDAK hastalarına ait dokulardan kendi laboratuvar koşullarımızda primer hücre kültürü yapılması amaçlanmıştır. Gereç ve Yöntem: KHDAK tanısı alan solunum fonksiyon testleri ve toraks bilgisayarlı tomografisi cerrahi müdahaleye uygun olan ve yazılı olarak onam veren hastalar çalışmaya dahil edilmiştir. Patolojik olarak doğrulanan tümör dokusu cerrahi rezeksiyon ile çıkarıldıktan sonra steril şartlarda mekanik ve kollajenaz/hyaluronidaz ile enzimatik parçalanmasıyla elde edilen hücreler Dulbecco’s Modified Eagle Medium (DMEM) içerisinde kültüre edilmiştir. Bulgular: Bu çalışmada tümör çapları 2*1.5*1.2, 8*7*6, 4*3.7*3 olan, metastazı olmayan ve yaşları 43-70 arasında değişen KHDAK tanısı alan 3 hastanın tümör dokularından kanser hücreleri başarılı bir şekilde izole edilip kültüre edilmiştir. Sonuç: Bu çalışma, literatürdeki bilgilere ve konuyla ilgili kişisel deneyimlere dayanarak akciğer kanserinde hasta kaynaklı tümör hücrelerinin elde edilebilmesi için önemli adımları içeren başarılı bir primer hücre kültürü protokolü sunmaktadır. Primer hücre kültürü laboratuvar ile hekimin klinik karar süreci arasında köprü oluşturarak akciğer kanserinin etkili bir şekilde tedavi edilebilmesi için ileride yapılacak çalışmalarla kişiselleştirilmiş tedavilerin geliştirilmesine katkı sağlayacağı düşünülen in vitro bir modeldir.

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Cite This Article

Yılmaz, S., Sarıkaya, M. D., Atar, E., Bağcı, B. Ş., Kenanoğlu, S., Mutlu, N., Yaşar, E., Önal, Ö., Canöz, Ö., Özkul, Y. (2023). Primary Culture of Cancer Cells Derived from Human Lung Tumors. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, Cilt: 39 Sayı: 1, 79–86. https://ajindex.com/articles/1266768

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