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Journals / Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi / 2023 / Cilt: 39 Sayı: 3

Electrochemical Profiling of a Promising Fused Pyrimidine-Triazole Drug Candidate

İlaç Adayı olarak Yeni Sentezlenen Pirimidin- Triazole Yapılı Bir Bileşiğin Elektrokimyasal Yapısının İncelenmesi

Fatma Kurul(DOKUZ EYLÜL ÜNİVERSİTESİ, İZMİR ULUSLARARASI BİYOTIP VE GENOM ENSTİTÜSÜ, BİYOTIP VE SAĞLIK TEKNOLOJİLERİ (YL) (TEZLİ) (İNGİLİZCE))0000-0002-6394-3079
Hüseyin İstanbullu(İZMİR KATİP ÇELEBİ ÜNİVERSİTESİ)
Seda Nur Topkaya Çetin(İZMİR KATİP ÇELEBİ ÜNİVERSİTESİ)0000-0002-7816-3155
Pages
475–484
Publication Date
2023-12-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results unveiled noteworthy changes in guanine basesfollowing CMCP interactions with both ssDNA and dsDNA, underscoring thepotential influence of this compound on DNA structure.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, a novel triazolopyrimidinone derivative, 5-(Chloromethyl)-2-(2-chlorophenyl)-[1,2,4]triazolo[1,5-a]pyrimidin-7(3H)-one, abbreviated CMCP,was synthesized as a viable therapeutic candidate.
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Key Findings & Data:We explored theexperimental parameters, including pH, concentration and scan rate to providebest analytical results.
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Conclusion & Impact:The results unveiled noteworthy changes in guanine basesfollowing CMCP interactions with both ssDNA and dsDNA, underscoring thepotential influence of this compound on DNA structure.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:İlaç Adayı · İlaç-DNA Etkileşimi · Triazolopirimidinon

Abstract

In this study, a novel triazolopyrimidinone derivative, 5-(Chloromethyl)-2-(2-chlorophenyl)-[1,2,4]triazolo[1,5-a]pyrimidin-7(3H)-one, abbreviated CMCP,was synthesized as a viable therapeutic candidate. Using electrochemical techniquessuch as Differential Pulse Voltammetry (DPV) and Cyclic Voltammetry (CV), weinvestigated its electrochemical characteristics and interactions with both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). We explored theexperimental parameters, including pH, concentration and scan rate to providebest analytical results. Our methodology involves analyzing changes inelectrochemical signals, specifically the peak current of guanine bases, before andafter interactions with the drug. Furthermore, stability tests were performed onCMCP over several days. The results unveiled noteworthy changes in guanine basesfollowing CMCP interactions with both ssDNA and dsDNA, underscoring thepotential influence of this compound on DNA structure.

Özet

Bu çalışmada, umut vadeden yeni bir ilaç adayı olarak triazolopirimidinone türevi olan 5-(klorometil)-2-(2-klorofenil)-[1,2,4]triazolo[1,5-a]pirimidin-7(3H)-on (kısaltılmış olarak CMCP) sentezlenmiştir. Diferansiyel Puls Voltametri (DPV) ve Döngüsel Voltametri (CV) gibi elektrokimyasal teknikler kullanılarak, elektrokimyasal özellikleri, ayrıca tek sarmal DNA (ssDNA) ve çift sarmal DNA (dsDNA) ile etkileşimleri araştırılmıştır. Optimum sonuçlar sağlamak için pH, konsantrasyon, tarama hızı ve immobilizasyon süresi gibi çeşitli deneysel parametreler araştırılmıştır. Çalışmada, ilaçla etkileşimden önce ve sonra guanin bazlarının pik akımı gibi elektrokimyasal sinyallerdeki değişiklikleri analiz edilmiştir. Ayrıca, CMCP üzerinde birkaç gün boyunca kararlılık testleri yapılmıştır. Sonuçlar, CMCP’nin hem ssDNA hem de dsDNA ile etkileşiminden sonra guanin bazlarında dikkat çekici değişiklikler olduğunu ortaya koymuştur ve bu bileşiğin DNA yapısı üzerindeki potansiyel etkisini vurgulamaktadır. Bu deneysel bulgular, CMCP’nin DNA üzerindeki etkisi nedeniyle ilerideki ilaç geliştirme çalışmaları için umut vadeden bir aday olduğu görüşünü desteklemektedir.

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Cite This Article

Kurul, F., İstanbullu, H., Çetin, S. N. T. (2023). Electrochemical Profiling of a Promising Fused Pyrimidine-Triazole Drug Candidate. Erciyes Üniversitesi Fen Bilimleri Enstitüsü Fen Bilimleri Dergisi, Cilt: 39 Sayı: 3, 475–484. https://ajindex.com/articles/1266761

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