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Journals / Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi / 2020 / Cilt: 7 Sayı: 2

Enzymatic Hydrolysis of Stingray (Dasyatis pastinaca) Muscle and Evaluation of Antioxidant and Copper Chelating Activities of the Hydrolysates

Vatoz (Dasyatis pastinaca) Balığı Kas Dokusunun Enzimatik Hidrolizi ve Hidrolizatların Antioksidan ve Bakır Şelatlama Aktivitelerinin Değerlendirilmesi

Ebru Kocadağ Kocazorbaz(EGE ÜNİVERSİTESİ)0000-0001-5611-5235
Meliha Kanatsızoğlu(EGE ÜNİVERSİTESİ)0000-0001-9547-5444
Hanife Ttuğçe Çeker(AKDENİZ ÜNİVERSİTESİ)0000-0001-8874-2241
Esra Menfaatli(EGE ÜNİVERSİTESİ)0000-0002-2370-6415
Pages
680–687
Publication Date
2020-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Moreover, the antioxidant (CUPRAC method) and metal chelating (copper chelating) activities of the hydrolysates were evaluated.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Bioactive peptides that originate from different sources, such as plant, animal, fungi, bacteria, and venom, are specific protein fragments that have positive effects on the body functions and health of living organisms.
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Key Findings & Data:Enzyme/substrate ratio, hydrolysis time, and degree were optimized.
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Conclusion & Impact:Moreover, the antioxidant (CUPRAC method) and metal chelating (copper chelating) activities of the hydrolysates were evaluated.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:biyoaktif peptid · enzimatik hidroliz · antioksidan

Abstract

Bioactive peptides that originate from different sources, such as plant, animal, fungi, bacteria, and venom, are specific protein fragments that have positive effects on the body functions and health of living organisms. Most of the discovered bioactive peptides from different protein sources showed various biological features, such as antihypertension, antioxidant, antithrombotic, antimicrobial, and anticancer immunomodulation. The aim of this study is the evaluation of biological activities of stingray (Dasyatis pastinaca) muscle tissue protein hydrolysate. Therefore, the homogenized muscle tissue of stingray was hydrolyzed by esperase and trypsin enzymes. Enzyme/substrate ratio, hydrolysis time, and degree were optimized. Moreover, the antioxidant (CUPRAC method) and metal chelating (copper chelating) activities of the hydrolysates were evaluated.

Özet

Biyoaktif peptidler canlıların vücut fonksiyonlarına ve sağlığına olumlu etkileri bulunan spesifik protein fragmentleri olup bitkisel, hayvansal, fungal, bakteriyel ve venom kaynaklı olabilmektedir. Farklı protein kaynaklarından keşfedilen biyoaktif peptidlerin çoğu, antihipertansiyon, antioksidan, antitrombotik, antimikrobiyal ve antikanser immünomodülasyon gibi çeşitli biyolojik özellikler göstermiştir. Bu çalışmanın amacı, vatoz (Dasyatis pastinaca) kas dokusu protein hidrolizatının biyolojik aktivitelerinin değerlendirilmesidir. Bu nedenle, vatozun homojenize kas dokusu, esperaz ve tripsin enzimleri tarafından hidrolize edildi. Enzim/substrat oranı, hidroliz süresi ve derecesi optimize edilerek hidrolizatların antioksidan (CUPRAC yöntemi) ve metal şelatlama (bakır şelatlama) aktiviteleri değerlendirildi.

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Cite This Article

Kocazorbaz, E. K., Kanatsızoğlu, M., Çeker, H. T., Menfaatli, E. (2020). Enzymatic Hydrolysis of Stingray (Dasyatis pastinaca) Muscle and Evaluation of Antioxidant and Copper Chelating Activities of the Hydrolysates. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, Cilt: 7 Sayı: 2, 680–687. https://doi.org/10.35193/bseufbd.665435

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