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Journals / Communications Faculty of Sciences University of Ankara Series C Biology / 2022 / Volume: 31 Issue: 2

Histochemical effects of brodifacoum on rat spleen

Burcu Bayramlı Öner(ANKARA UNIVERSITY, INSTITUTE OF BIOTECHNOLOGY, BASIC BIOTECHNOLOGY (DR))0000-0002-7959-5179
Nursel Gül(ANKARA UNIVERSITY, FACULTY OF SCIENCE)0000-0003-2978-4163
Pages
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Publication Date
2022-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of this study, it was found that Brodifacoum caused immunohistochemical abnormalities in the rat spleen, affected the morphological structure of CD4 and CD8 T lymphocytes and created an immune response in rats.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the histochemical effects of Brodifacoum, an anticoagulant used against rodents, on the spleen are examined under a light microscope using CD4 and CD8 histochemical staining methods.
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Key Findings & Data:A single dose of 0.2 mg Brodifacoum was dissolved in Dimethyl Sulfoxide (DMSO) and was given orally to mature male rats.
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Conclusion & Impact:As a result of this study, it was found that Brodifacoum caused immunohistochemical abnormalities in the rat spleen, affected the morphological structure of CD4 and CD8 T lymphocytes and created an immune response in rats.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:brodifacoum · Rattus norvegicus · spleen

Abstract

In this study, the histochemical effects of Brodifacoum, an anticoagulant used against rodents, on the spleen are examined under a light microscope using CD4 and CD8 histochemical staining methods. A single dose of 0.2 mg Brodifacoum was dissolved in Dimethyl Sulfoxide (DMSO) and was given orally to mature male rats. Spleen samples were collected under ether anesthesia after 24 h, 72 h, 14 days, and 30 days from the rats in the experimental groups and after 14 days from the rats in the control group. In this light microscope study, it was observed that the capsule, white pulp, and red pulp zones in the rat spleen were constructed normally and as their natural structures primary and secondary follicles (germinal center) they were few, and CD4 and CD8 lymphocytes were spherically structured. In the 24 h spleens of the rats, the diameters of germinal centers were expanded and deterioration of the structure of CD4 and CD8 cells was observed. Related to the increase in time (72 h and 14 days) it was determined that primary follicles increased in number and the diameters of germinal centers expanded. In addition to this, after30 days, the rate of CD4:CD8 of the brodifacoum applied rat spleens were approximately the rate of the control group, and the improvement of the structures of the cells was reported as an effect of regeneration. As a result of this study, it was found that Brodifacoum caused immunohistochemical abnormalities in the rat spleen, affected the morphological structure of CD4 and CD8 T lymphocytes and created an immune response in rats. It is thought that the obtained results will be a source for the studies on Brodifacoum.

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Cite This Article

Öner, B. B., Gül, N. (2022). Histochemical effects of brodifacoum on rat spleen. Communications Faculty of Sciences University of Ankara Series C Biology, Volume: 31 Issue: 2. https://doi.org/10.53447/communc.1168968

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