Journals / Kocatepe Veteriner Dergisi / 2019 / Cilt: 12 - Sayı: 1

Levels of Serum 25 (OH) D3 in Naturally Infected Lambs With Giardia duodenalis

Giardia duodenalis ile Doğal Enfekte Kuzularda Serum 25 (OH) D3 Seviyeleri

Pages
71–74
Publication Date
2019-03-31
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Statistically significant changes were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Giardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals.
📊
Key Findings & Data:The aim of this study was to determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis.
💡
Conclusion & Impact:Statistically significant changes were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Giardiasis · kuzu · 25-hidroksi vitamin D3

Abstract

Giardia duodenalis is a binuclear flagellated protozoan that can infect birds, reptiles and many mammals. The pathogen is one of the most important causes of diarrhea in humans and farm animals. The aim of this study was to determine the changes in serum 25-hydroxyvitamin D3 levels in naturally infected lambs with Giardia duodenalis. The animal material of the study was used Sakız sheep which had naturally infected with Giardia duodenalis between 15-35 days of age from the Aydın region (n = 30), healthy lambs in the same age range (n = 15) and both sexes. Sterile stool samples with the method of rectal touching of fecal samples from infected and healthy animals, blood samples were obtained in a total of 5 ml of v.jugularis according to the technique in order to determine serum 25-hydroxyvitamin D3 levels. Giardia screening was performed by direct microscopic examination of stool samples. 25-OH-D levels were measured with Savant brand fluorescent immunoassay device from the obtained serum samples. Mann-Whitney U test was used in statistical analysis. Statistically significant changes were observed in 25-hydroxyvitamin D3 levels of healthy and natural infected animals (p <0.05).

Özet

Giardia duodenalis kuşlar, reptiller ve bir çok memeli hayvanı enfekte edebilen binükleuslu flagellalı bir protozoondur. Söz konusu patojen insanlarda ve çiftlik hayvanlarında başta olmak üzere şekillenen ishalin en önemli nedenleri arasında yer almaktadır. Çalışmada Giardia duodenalis ile doğal enfekte kuzularda serum 25-hidroksi vitamin D3 seviyelerindeki değişimlerin belirlenmesi amaçlanmıştır. Araştırmanın hayvan materyalini Aydın bölgesinde bulunan yaşları 15-35 günlük arasında her iki cinsiyetten sakız ırkı Giardia duodenalis ile doğal enfekte (n=30), benzer yaş aralığındaki aynı ırktan (n=15) ve her iki cinsiyetten sağlıklı kuzular oluşturdu. Enfekte ve sağlıklı hayvanlardan dışkı örnekleri rektal tuşe yöntemi ile steril dışkı örnekleri, serum 25-hidroksi vitamin D3 seviyelerinin belirlenmesi amacı ile de tekniğine uygun olarak v.jugularisden toplamda 5 ml olacak şekilde kan örnekleri alındı. Dışkı örneklerinden direkt mikroskobik bakı yöntemi ile giardia taraması yapıldı. Elde edilen serum örneklerinden Savant marka flöresan immunoassay cihazı ile 25-OH-D seviyeleri ölçüldü. İstatistiksel analizde Mann-Whitney U testinden yararlanıldı. Sağlıklı ve doğal enfekte hayvanlara ait 25-hidroksi vitamin D3 seviyeleri arasında istatistiksel (p<0,05) anlamlı değişimlerin olduğu belirlendi.

🔖

Cite This Article

CAMKERTEN, G., ERDOĞAN, H., URAL, D. A., CAMKERTEN, İ., ERDOĞAN, S., URAL, K. (2019). Levels of Serum 25 (OH) D3 in Naturally Infected Lambs With Giardia duodenalis. Kocatepe Veteriner Dergisi, Cilt: 12 - Sayı: 1, 71–74. https://ajindex.com/articles/125038

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Astiazaran-Garcia H, Lopez-Teros V, Valencia ME, Vazquez-Ortiz F, Sotelo-Cruz N, Quihui-Cota L. Giardia lamblia infection and its implications for vitamin A liver stores in school children. Annals of Nutrition and Metabolism. 2010;57(3-4): 228-233.
  2. Aytuğ CN, Alaçam E, Özkoç Ü, Yalçın BC. Koyun-Keçi Hastalıkları ve Yetiştiriciliği, İstanbul: Teknografik Matbaası, 1990; 212-213.
  3. Baljer G, Wieler L. Ätiologie. Pathogenese und Immunprophylaxe der neonatalen Durchfallerkrankungen der Kälber. Vet 1989; 5: 18-26. Bilal T. Koyun-Keçilerin İç Hastalıkları ve Beslenmesi. İstanbul Üniversitesi Yayın No: 4593, İstanbul: Veteriner Fakültesi Yayın No: 24, 2005. Buret AG. Pathophysiology of enteric infections with Giardia duodenalis. Parasite. 2008;15(3):261-265.
  4. Cordingley FT, Crawford GPM. Giardia infection causes vitamin B12 deficiency. Australian and New Zealand journal of medicine. 1986; 16(1): 78-79. Dignass AU. Mechanisms and modulation of intestinal epithelial repair. Inflamm Bowel Dis. 2001;7: 68 –77. Gaydos J. Giardia and Wildlife South Coop Wild Dis St Br 1998; 14:2. Geurden T, Pohleb H, Sarrea C, Dreesena L, Vercruyssea J, Claerebouta E. The efficacy of a treatment with fenbendazole against an experimental Giardia duodenalis infection in lambs. Small Rum Res. 2011; 96(2-3):211-215.
  5. Geurden T, Vercruysse J, Claerebout E. Is giardia a significant pathogen in production animals?. Exp Parasitol. 2009; 124:98-106.
  6. Gökçe E, Ünver A, Erdoğan HM. İshalli neonatal kuzularda enterik patojenlerin belirlenmesi. Kafkas Univ Vet Fak Derg.2010; 16:717-722.
  7. Hall GA, Jones PW, Morgan JH. Calf diarrhoea. Bovine Medicine, In: Andrews AH. (Editors). Diseases and Husbuandry of Cattle. Berlin: Blackwell, 1996; 154-180.
  8. Hassan V, Hassan S, Seyed-Javad P, Ahmad K, Asieh H, Maryam S, Farid F, Siavash A. Association Between Serum 25 (OH) Vitamin D Concentrations and Inflammatory Bowel Diseases (IBDs) Activity. Medical Journal of Malaysia. 2013; 68(1): 34-38.
  9. Kong J, Zhang Z, Musch MW, Ning G, Sun J, Hart J, Bissonnette M, Li YC. Novel role of the vitamin D receptor in maintaining the integrity of the intestinal mucosal barrier. Am J Physiol Gastrointest Liver Physiol. 2008; 294(1):208-16.
  10. Lim WC, Hanauer SB, Li YC. Mechanisms of Disease: Vitamin D and Inflammatory Bowel Disease. Nature Clinical Practice Gastroenterology and Hepatology.2005; 2(2):103-111.
  11. Lim WC, Hanauer SB, Li YC. Mechanisms of disease: vitamin D and inflammatory bowel disease. Nature Clin Pract Gastroenterol Hepatol