SavedAdd your journal

Journals / Kocatepe Veteriner Dergisi / 2018 / Cilt: 11 - Sayı: 4

Investigation of Possible Polymorphisms in BMPR1B, BMP15 and GDF9 Genes in Pırlak Sheep

Pırlak Koyunlarında BMPR1B, BMP15 ve GDF9 Genlerinde Olası Polimorfizmlerin Araştırılması

Koray ÇELİKELOĞLU
Metin ERDOĞAN
Özlem HACAN
Serdar KOÇAK
Zehra BOZKURT
Mustafa TEKERLİ
Pages
356–362
Publication Date
2018-12-15
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The PCR and DNA sequencing analyzes carried out on the basis of the respective genes revealed that Pırlaks are monomorphic in the aspect of above mentioned genes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was realised to determine the presence of polymorphism in major proliferacy genes named Bone Morphogenetic Protein Receptor type 1B (BMPR1B), Bone Morphogenetic Protein 15 (BMP15) and Growth Differentiation Factor 9 (GDF9) of 8 uniparous and 8 multiparous Pırlak sheep reared at Sheep and Goats Breeders Association’s Breeding Station, Afyonkarahisar between 2016 and 2017.
📊
Key Findings & Data:Blood samples collected for using in genetic studies were taken from V. jugularis of animals into EDTA vacuum tubes and DNA isolations were performed using genomic DNA isolation kit.
💡
Conclusion & Impact:The PCR and DNA sequencing analyzes carried out on the basis of the respective genes revealed that Pırlaks are monomorphic in the aspect of above mentioned genes.
🔬Design:Research Article
📍Scope:2016 Verileri
⚙️Variables:Pırlak · Döl verimi · BMPR 1B

Abstract

This study was realised to determine the presence of polymorphism in major proliferacy genes named Bone Morphogenetic Protein Receptor type 1B (BMPR1B), Bone Morphogenetic Protein 15 (BMP15) and Growth Differentiation Factor 9 (GDF9) of 8 uniparous and 8 multiparous Pırlak sheep reared at Sheep and Goats Breeders Association’s Breeding Station, Afyonkarahisar between 2016 and 2017. Blood samples collected for using in genetic studies were taken from V. jugularis of animals into EDTA vacuum tubes and DNA isolations were performed using genomic DNA isolation kit. The PCR and DNA sequencing analyzes carried out on the basis of the respective genes revealed that Pırlaks are monomorphic in the aspect of above mentioned genes.

Özet

Bu araştırmada, Afyonkarahisar İli Damızlık Koyun-Keçi Yetiştiricileri Birliği damızlık istasyonunda 2016 ve 2017 yıllarında çoklu ve tekli doğum yapanlardan sekizer baş olmak üzere toplam 16 baş Pırlak koyunda Bone Morphogenetic Protein Receptor type 1B (BMPR1B), Bone Morphogenetic Protein 15 (BMP15) ve Growth Differentiation Factor 9 (GDF9) genlerindeki polimorfizmin varlığı ve bunun tespit edilmesi durumunda çoklu doğumla ilişkilendirilmesi amaçlanmıştır. Genetik çalışmalarda kullanılmak üzere toplanan kan örnekleri hayvanların V. jugularis’lerinden EDTA’lı vakumlu tüplere alınmış ve genomik DNA izolasyon kiti kullanılarak DNA izolasyonları yapılmıştır. İlgili genler yönünden gerçekleştirilen PCR ve DNA dizileme analizleri sonucunda her üç gen yönünden de Pırlakların monomorfik yapıda olduğu ortaya konulmuştur.

🔖

Cite This Article

ÇELİKELOĞLU, K., ERDOĞAN, M., HACAN, Ö., KOÇAK, S., BOZKURT, Z., TEKERLİ, M. (2018). Investigation of Possible Polymorphisms in BMPR1B, BMP15 and GDF9 Genes in Pırlak Sheep. Kocatepe Veteriner Dergisi, Cilt: 11 - Sayı: 4, 356–362. https://ajindex.com/articles/124934

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (7)

  1. Alink F M, Mylne MJA, Watt RG, Kenyon P, Wood MJ, McEvoy TG. Inverdale fecundity gene (FecXI) influences twin ovulation incidence in pubertal ewe lambs from Texel sires and Cheviot or Scottish 2005; http://www.bsas.org.uk/wp-content/themes/bsas/proceedings/Pdf2005/055.pdfBlackface dams, 15.01.2016.
  2. Bodin L, Di Pasquale E, Fabre S, Bontoux M, Monget P, Persani L, Mulsant P. A Novel Mutation in the Bone Morphogenetic Protein 15 Gene Causing Defective Protein Secretion Is Associated with Both Increased Ovulation Rate and Sterility in Lacaune Sheep. Endocrinology, 2007; vol. 148(1), ss. 393–400.
  3. Boom R, Sol CJA, Salimans MMM, Jansen CL, Wertheim Van Dillen PME, Van der Noordaa J. Rapid and simple method for purification on nucleic acids. Journal of Clinical Microbiology, 1990; ss.495-503.Çelikeloğlu K. Pırlak Kuzularında Büyüme Eğrilerini Etkileyen Genetik ve Çevresel faktörlerin belirlenmesi ve eğri parametreleri yönünden baba koçların değerlendirilmesi, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyonkarahisar, 2012.
  4. Davis GH, Montgomery GW, Allison AJ, Kelly RW, Bray AR. Segregation of a major gene influencing fecundity in progenyof Booroola sheep. New Zealand Journal of Agricultural Research, 1982; vol. 25, ss. 525–529.
  5. Davis GH, Galloway SM, Ross IK, Gregan SM, Ward J, Nimbkar BV, Ghalsasi PM, Nimbkar B, Gray GD, Subandriyo I, Tiesnamurti B, Martyniuk E, Eythorsdottir E, Mulsant P, Lecerf F, Hanrahan JP, Bradford GE, Wilson T. DNA tests in prolific sheep from eight countries provide new evidence on origin of the Booroola (FecB) mutation. Biology of Reproduction, 2002; vol. 66, ss. 1869–1874. Davis GH. Major genes affecting ovulation rate in sheep. Genetics Selection Evolution, 2005; vol. 37, ss.11–23.
  6. Davis GH, Balakrishnan L, Ross IK, Wilson T, Galloway SM, Lumsden BM, Hanrahan JP, Mullen M, Mao XZ, Wang GL, Zhao ZS, Zeng YQ, Robinson JJ, Mavrogenis AP, Papachristoforou C, Peter C, Baumung R, Cardyn P, Boujenane I, Cockett NE, Eythorsdottir E, Arranz JJ, Notter D. Investigation of the Booroola (FecB) and Inverdale (FecXI) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Animal Reproduction Science, 2006; vol. 92, ss. 87–96.
  7. Demars J, Fabre S, Sarry J, Rossetti R, Gilbert H, Persani L,. & Martyniuk E. Genome-wide association studies identify two novel BMP15 mutations responsible for an atypical hyperprolificacy phenotype in sheep. PLoS genetics, 2013; 9(4), e1003482. Dodds KG, Davis GH, Elsen JM, Isaacs KL, Owe