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Journals / Kocatepe Veteriner Dergisi / 2019 / Cilt: 12 - Sayı: 4

Prevalence of Methicillin Resistance and Panton-Valentine Leukocidin Genes in Staphylococci Isolated from Pirlak Sheep with Subclinical Mastitis in Turkey

Türkiye'de Subklinik Mastitisli Pırlak Koyunlardan İzole Edilen Stafilokoklarda Metisilin Direnç ve Panton-Valentine Lökosidin Genlerinin Prevalansı

Esra ŞEKER
Erhan ÖZENÇ
Duygu BAKİ ACAR
Müesser YILMAZ
Pages
424–429
Publication Date
2019-12-31
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

To our knowledge, this is the first study showing the presence of mecA and pvl genes in the Staphylococci isolated from Pirlak sheep with subclinical mastitis in Turkey.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to investigate the presence of mecA and pvl genes in 47 Staphylococci previously isolated from 464 half-udder milk samples belong to 235 Pirlak sheep screening for subclinical mastitis.
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Key Findings & Data:The species from Pirlak sheep used in the present study included: 13 S. aureus, 13 S. epidermidis, six S. xylosus, five S. chromogenes, three S. simulans, three S. hyicus, two S. warneri, one S. lentus and one S. saprophyticus.
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Conclusion & Impact:To our knowledge, this is the first study showing the presence of mecA and pvl genes in the Staphylococci isolated from Pirlak sheep with subclinical mastitis in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Koyun · Mastitis · Metisilin Direnci

Abstract

This study aimed to investigate the presence of mecA and pvl genes in 47 Staphylococci previously isolated from 464 half-udder milk samples belong to 235 Pirlak sheep screening for subclinical mastitis. The species from Pirlak sheep used in the present study included: 13 S. aureus, 13 S. epidermidis, six S. xylosus, five S. chromogenes, three S. simulans, three S. hyicus, two S. warneri, one S. lentus and one S. saprophyticus. A total of 10 strains (21.3%) were determined to harbour mecA gene, of these, two (4.2%) also contained pvl gene. The strains carrying mecA gene were found to be S. aureus (3/13), S. xylosus (3/6), S. epidermidis (2/13), S. lentus (1/1) and S. hyicus (1/3). The presence of pvl gene was determined in a total of eight strains (17.0%), six (12.8%) of these were alone. Of pvl positive strains, three, three, one, and one were S. aureus, S. xylosus, S. simulans and S. hyicus, respectively. To our knowledge, this is the first study showing the presence of mecA and pvl genes in the Staphylococci isolated from Pirlak sheep with subclinical mastitis in Turkey.

Özet

Bu çalışmada, subklinik mastitis yönünden taranan 235 Pırlak koyuna ait 464 meme lobu süt örneğinden daha önce izole edilen 47 Stafilokok türünde mecA ve pvl genlerinin varlığının araştırılması amaçlandı. Çalışmada, Pırlak koyunlardan izole edilen 13 S. aureus, 13 S. epidermidis, altı S. xylosus, beş S. chromogenes, üç S. simulans, üç S. hyicus, iki S. warneri, bir S. lentus ve bir S. saprophyticus suşu kullanıldı. Toplam 10 suşun (%21,3) mecA geni taşıdığı, bunlardan ikisinin (%4,2) ayrıca pvl genine de sahip olduğu belirlendi. mecA geni taşıyan suşlar S. aureus (3/13), S. xylosus (3/6), S. epidermidis (2/13), S. lentus (1/1) ve S. hyicus (1/3) olarak bulundu. Toplam sekiz suşta (%17,0) pvl geni belirlenirken, bunlardan altısının (%12,8) bu geni tek başına taşıdığı tespit edildi. pvl pozitif suşların üçü S. aureus, üçü S. xylosus, biri S. simulans ve biri S. hyicus olarak belirlendi. Bilgimize göre, bu çalışma Türkiye'de subklinik mastitisli Pırlak koyunlardan izole edilen Stafilokoklarda mecA ve pvl genlerinin varlığını gösteren ilk çalışmadır.

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Cite This Article

ŞEKER, E., ÖZENÇ, E., ACAR, D. B., YILMAZ, M. (2019). Prevalence of Methicillin Resistance and Panton-Valentine Leukocidin Genes in Staphylococci Isolated from Pirlak Sheep with Subclinical Mastitis in Turkey. Kocatepe Veteriner Dergisi, Cilt: 12 - Sayı: 4, 424–429. https://ajindex.com/articles/124699

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