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Journals / Kocatepe Veteriner Dergisi / 2020 / Cilt: 13 - Sayı: 4

Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality

Farklı Partenogenetik Aktivasyon Sürelerinin Fare Embriyo Gelişimi ve Kalitesi Üzerine Etkileri

Ali TAŞKIN
Nilhan COŞKUN
Ahmet KOCABAY
Pages
383–387
Publication Date
2020-12-31
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

The results in the present study suggested that the parthenogenetic activation of the 6 hour activation period was found to be higher than at 3, 4 and 5 hours.Keywords: Mouse, Oocyte, Parthenogenetic, Activation period

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study we investigate the effects of parthenogenetic activation on in vitro embryo development and quality in different activation periods. oocytes were obtained 14 hours after human chorionic gonadotropin (hCG) injection from superovulated B6D2F1 female mice then parthenogenetic activation started 18 hours after hCG injection.
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Key Findings & Data:The oocytes were activated at different activation periods for 3, 4, 5 or 6 hours in 10 mM strontium chloride (SrCl2) + 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing a Ca 2+ free Chatot Ziomek Brinster (CZB) activation medium, followed by further incubation for two hours at 37°C and 5% CO2 in embryo culturing medium + TSA.
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Conclusion & Impact:The results in the present study suggested that the parthenogenetic activation of the 6 hour activation period was found to be higher than at 3, 4 and 5 hours.Keywords: Mouse, Oocyte, Parthenogenetic, Activation period
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Fare · Oosit · Partenogenetik

Abstract

In the present study we investigate the effects of parthenogenetic activation on in vitro embryo development and quality in different activation periods. oocytes were obtained 14 hours after human chorionic gonadotropin (hCG) injection from superovulated B6D2F1 female mice then parthenogenetic activation started 18 hours after hCG injection. The oocytes were activated at different activation periods for 3, 4, 5 or 6 hours in 10 mM strontium chloride (SrCl2) + 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing a Ca 2+ free Chatot Ziomek Brinster (CZB) activation medium, followed by further incubation for two hours at 37°C and 5% CO2 in embryo culturing medium + TSA. The results in the present study suggested that the parthenogenetic activation of the 6 hour activation period was found to be higher than at 3, 4 and 5 hours.Keywords: Mouse, Oocyte, Parthenogenetic, Activation period

Özet

Çalışmamızın amacı, partenogenetik aktivasyonda farklı aktivasyon sürelerinin in vitro embriyo gelişimi ve kalitesi üzerindeki etkilerinin araştırılmasıdır. Superovule B6D2F1 ırkı dişi farelere uygulanan insan koryonik gonadotropin (hCG) enjeksiyonundan 14 saat sonra oositler elde edildi ve 18 saat sonra partenogenetik aktivasyona başlandı. Oositler, 10 mM stronsiyum klorür (SrCl2) + 5 μg/mL-1 sitokalazin B (CB) + 5 nM trikostatin A (TSA) Ca 2+ içermeyen Chatot Ziomek Brinster (CZB) medyumu içerisinde 3, 4, 5 ve 6 saat bekletildi. Aktivasyon sonrası, embriyo kültür medyumu + TSA’da inkübatörde 37°C ve %5 CO2 ortamında 2 saat bekletildi. Son olarak, tüm embriyolar 120 saat süre ile kültüre edildi. Bu çalışmadan elde edilen sonuçlar göre, 6 saatlik partenogenetik aktivasyon başarısının, 3, 4 ve 5 saatlik sürelere göre daha yüksek olduğu saptandı.

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Cite This Article

TAŞKIN, A., COŞKUN, N., KOCABAY, A. (2020). Effects of Different Parthenogenetic Activation Periods on Mouse Embryo Development and Quality. Kocatepe Veteriner Dergisi, Cilt: 13 - Sayı: 4, 383–387. https://ajindex.com/articles/124634

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