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Journals / Kocatepe Veteriner Dergisi / 2019 / Cilt: 12 - Sayı: 3

Investigation of Antibacterial Activities of Ethanol and Methanol Extracts of Some Marine Algae Species on Yersinia ruckeri

Bazı Deniz Alg Türlerinin Etanol ve Metanol Ekstraktlarının Antibakteriyel Aktivitelerinin Yersinia ruckeri Üzerinde Araştırılması

Jale KORUN
Emine Şükran OKUDAN
Remziye Eda YARDIMCI
Gülşen TİMUR
Aycan ULUTAŞ
Mehmet GÖKOĞLU
Yağmur Saadet ÇELİK
Beytullah Ahmet BALCI
Pages
226–234
Publication Date
2019-09-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The strains isolated from the sick fish were identified as Yersinia ruckeri according to the results of the phenotypic tests, the API 20E rapid diagnostic kit and PCR.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, antibacterial activities of extracts of Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) and Sargassum vulgare (Ocrophyta) on Yersinia ruckeri strains isolated from sick rainbow trout (Oncorhynhus mykiss) were evaluated.
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Key Findings & Data:The strains isolated from the sick fish were identified as Yersinia ruckeri according to the results of the phenotypic tests, the API 20E rapid diagnostic kit and PCR.
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Conclusion & Impact:In conclusion, although it was reported that algae had antibacterial activities, it was understood that every algae species could not exhibit antibacterial activity.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Gökkuşağı alabalığı · Yersinia ruckeri · deniz algleri

Abstract

In this study, antibacterial activities of extracts of Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) and Sargassum vulgare (Ocrophyta) on Yersinia ruckeri strains isolated from sick rainbow trout (Oncorhynhus mykiss) were evaluated. Also, susceptibilities of the strains against various antibiotics were studied. The strains isolated from the sick fish were identified as Yersinia ruckeri according to the results of the phenotypic tests, the API 20E rapid diagnostic kit and PCR. In the study, it was found that the methanol extracts of L. ceranoides, H. scoparia, P. pavonica and S. vulgare did not show antibacterial activity against Y. ruckeri. The ethanol extract of P. pavonica showed low antibacterial activity against Y. ruckeri. The ethanol extracts of L. ceranoides, H. scoparia and S. vulgare macroalgae did not show antibacterial activity against the bacterium. According to the results of the antibiogram test, it was found that the strains were sensitive against ampicillin, flumequine, oxytetracycline and trimethoprime. The strains showed intermediate resistance against erythromycine. In conclusion, although it was reported that algae had antibacterial activities, it was understood that every algae species could not exhibit antibacterial activity.

Özet

Bu çalışmada Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) ve Sargassum vulgare (Ocrophyta) alglerinin etanol ve metanol ekstraktlarının hasta gökkuşağı alabalığı (Oncorhynhus mykiss)’ndan izole edilen Yersinia ruckeri suşları üzerine antibakteriyel aktiviteleri değerlendirilmiştir. Ayrıca, suşların çeşitli antibiyotiklere duyarlılıkları da çalışılmıştır. Balıklardan izole edilen suşlar fenotipik testler, API 20E hızlı tanı kiti ve PZR sonuçlarına göre Yersinia ruckeri olarak tanımlanmıştır. Çalışmada L. ceranoides, H. scoparia, P. pavonica ve S. vulgare makroalglerinin metanol ekstraktlarının Y. ruckeri’ye karşı antibakteriyel etki göstermediği bulunmuştur. P. pavonica’nın etanol ekstraktı Y. ruckeri’ye karşı düşük antibakteriyel aktivite göstermiştir. L. ceranoides, H. scoparia ve S. vulgare makroalglerinin etanol ekstraktlarının bakteriye karşı antibakteriyel aktivite göstermediği tespit edilmiştir. Antibiyogram test sonuçlarına göre suşların ampisilin, flumekuin, oksitetrasiklin ve trimetoprime duyarlı oldukları bulunmuştur. Suşlar eritromisine karşı orta derecede direnç göstermiştir. Sonuç olarak, alglerin antibakteriyel aktiviteye sahip olduklarının bildirilmesine karşın her alg türünün antibakteriyel aktivite gösteremeyeceği anlaşılmıştır.

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Cite This Article

KORUN, J., OKUDAN, E. Ş., YARDIMCI, R. E., TİMUR, G., ULUTAŞ, A., GÖKOĞLU, M., ÇELİK, Y. S., BALCI, B. A. (2019). Investigation of Antibacterial Activities of Ethanol and Methanol Extracts of Some Marine Algae Species on Yersinia ruckeri. Kocatepe Veteriner Dergisi, Cilt: 12 - Sayı: 3, 226–234. https://ajindex.com/articles/124625

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