SavedAdd your journal

Journals / Kocatepe Veteriner Dergisi / 2020 / Cilt: 13 - Sayı: 3

In Vitro Antimicrobial Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep with Caseous Lymphadenitis

Kazeöz Lenfadenitisli Koyunlardan İzole Edilen Corynebacterium pseudotuberculosis Suşlarının in-Vitro Antibiyotik Duyarlılıkları

Ziya İLHAN
Pages
267–271
Publication Date
2020-09-30
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

None of the isolates were susceptible to streptomycin.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis, is a highly prevalent disease of sheep and goats resulting in significant economic losses in the world.
📊
Key Findings & Data:A total of 16 C. pseudotuberculosis strains isolated from lung samples of 67 sheep with CLA lesions were tested.
💡
Conclusion & Impact:None of the isolates were susceptible to streptomycin.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Antibiyotik duyarlılık testi · Corynebacterium pseudotuberculosis · Kazeöz lenfadenitis

Abstract

Caseous lymphadenitis (CLA), caused by Corynebacterium pseudotuberculosis, is a highly prevalent disease of sheep and goats resulting in significant economic losses in the world. In this study, it was aimed to determine the in vitro antimicrobial susceptibility of C. pseudotuberculosis strains isolated from sheep with CLA to various antibiotics. A total of 16 C. pseudotuberculosis strains isolated from lung samples of 67 sheep with CLA lesions were tested. Tetracycline, oxytetracycline, streptomycin, enrofloxacin, cloxacillin, ampicillin/sulbactam, amoxicillin/clavulanic acid, penicillin/novobiocin, trimethoprim/sulfamethoxazole and neomycin/bacitracin/tetracycline discs were tested by disc diffusion method. A total of 14 (87.5%) C. pseudotuberculosis strains were susceptible to enrofloxacin, 13 (81.2%) strains to neomycin/bacitracin/tetracycline, 12 (75.0%) strains to penicillin/novobiocin, 11 (68.7%) strains to oxytetracycline, 11 (68.7%) strains to amoxicillin/clavulanic acid, 10 (62.5%) strains to cloxacillin, 9 (56.2%) strains to tetracycline, 5 (31.2%) strains to ampicillin/sulbactam and 3 (18.7%) strains to trimethoprim/sulfamethoxazole. None of the isolates were susceptible to streptomycin.

Özet

Koyun ve keçilerde Corynebacterium pseudotuberculosis tarafından oluşturulan kazeöz lenfadenitis (KLA), tüm dünyada yaygın olarak görülmekte ve önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, KLA’li koyunlardan izole edilen C. pseudotuberculosis suşlarının çeşitli antibiyotiklere in vitro duyarlılıklarının belirlenmesi amaçlandı. Çalışmada KLA lezyonları gösteren toplam 67 adet koyunun akciğerlerinden izole edilen 16 adet C. pseudotuberculosis suşu test edildi. Disk difüzyon yöntemiyle yapılan değerlendirmede; tetrasiklin, oksitetrasiklin, streptomisin, enrofloksasin, kloksasilin, ampisilin/sulbaktam, amoksisilin/klavulanik asit, penisilin/novobiosin, trimetoprim/sulfametaksazol ve neomisin/basitrasin/tetrasiklin diskleri kullanıldı. Suşların 14 (%87.5) adeti enrofloksasine, 13 (%81.2) adeti neomisin/basitrasin/tetrasikline, 12 (%75.0) adeti penisilin/novobiosine, 11 (%68.7) adeti oksitetrasikline, 11 (%68.7) adeti amoksisilin/klavulanik asite, 10 (%62.5) adeti kloksasiline, 9 (%56.2) adeti tetrasikline, 5 (%31.2) adeti ampisilin/sulbaktama ve 3 (%18.7) adeti ise trimetoprim/sulfametaksazole duyarlı bulundu. İzolatlardan hiçbiri streptomisine duyarlı bulunmadı.

🔖

Cite This Article

İLHAN, Z. (2020). In Vitro Antimicrobial Susceptibility of Corynebacterium pseudotuberculosis Isolated from Sheep with Caseous Lymphadenitis. Kocatepe Veteriner Dergisi, Cilt: 13 - Sayı: 3, 267–271. https://ajindex.com/articles/124622

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (8)

  1. Abdel Wahab MB, Shigidi MTA. In vitro sensitivity of Corynebacterium pseudotuberculosis to 16 antimicrobial agents. Sudan J Vet Res. 2013; 28: 23-25.
  2. Abebe D, Tessema TS. Determination of Corynebacterium pseudotuberculosis prevalence and antimicrobial susceptibility pattern of isolates from lymph nodes of sheep and goats at an organic export abattoir, Modjo, Ethiopia. Lett Appl Microbiol. 2015; 61: 469-476.
  3. Alavi SM, Alavi L. Treatment of brucellosis: a systematic review of studies in recent twenty years. Caspian J Intern Med. 2013; 4(2): 636–641. Algammal AM. Molecular characterization and antibiotic susceptibility of Corynebacterium pseudotuberculosis isolated from sheep and goats suffering from caseous lymphadenitis. Zagazig Vet J. 2016; 44 (1): 1-8.
  4. Ali AD, Mahmoud AA, Khadr AM, Elshemey TM, Abdelrahman AH. Corynebacterium pseudotuberculosis: disease prevalence, lesion distribution, and diagnostic comparison through microbiological culture and molecular diagnosis. AJVS. 2016; 51(2): 189-198.
  5. Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibilty testing by a standardized single disk method. Am J Clin Pathol. 1966; 45: 493. Bastos BL, Portela RWD, Dorella FA, Ribeiro D, Seyffert N, Castro TLP, Miyoshi A, Oliveira SO, Meyer R, Azevedo V. Corynebacterium pseudotuberculosis: immunological responses in animal models and zoonotic potential. J Clin Cell Immunol. 2012; S4:005: doi:10.4172/2155-9899.S4-005. Dorella FA, Pacheco LGC, Olivera SC, Miyashi A, Azevedo V. Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence. Vet Res. 2006; 37: 201-218. Fernández EP, Vela AI, Las Heras A, Domínguez L, Fernández-Garayzábal JF, Moreno MA. Antimicrobial susceptibility of corynebacteria isolated from ewe’s mastitis. Int J Antimicrob Agents. 2001; 18: 571– 574. İlhan Z. Koyunlarda Corynebacterium pseudotuberculosis’in ELISA ve dot-blot ELISA ile teşhisi. Turk J Vet Anim Sci. 2003; 27(6): 1327-1333.
  6. Kuria JK, Holstad G. A seroepidemiological investigation of Corynebacterium pseudotuberculosis infection in sheep flocks in southern Norway. Acta Vet Scand. 1989; 30: 107–108.
  7. Lee S, Siddiqui R, Khan NA. Animals living in polluted environments are potential source of antimicrobials against infectious agents. Pathogens Glob Health. 2012; 106 (4): 218-223.
  8. Middleton MJ, Epstein WM, Gregory GG. Caseous lymphadenitis on Flinders Island: prevalence and management surveys. Aust Vet J. 1991; 68: 311–312. Muc